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Control of Virus Diseases of Plants 16316–20 kDa as determ<strong>in</strong>ed by gel-filtration chromatography (Verma et al.,1979). The prote<strong>in</strong> has a PI of around 9 and has a molecular weight of 30 kDa(Srivastava, 1995). The RIP was found to be extremely thermostable (Vermaand Awasthi, 1979). Follow<strong>in</strong>g treatment with the systemic RIP, the host producesa VIA. The VIA shows characteristics of a prote<strong>in</strong> and reduces <strong>in</strong>fectivityof the viruses both <strong>in</strong> vitro and <strong>in</strong> vivo (Verma and Awasthi, 1980). Upongel filteration on Sephadex G-25, two active fractions exhibit<strong>in</strong>g prote<strong>in</strong>characteristics were recovered (Verma and Awasthi, 1980).The prote<strong>in</strong> occurr<strong>in</strong>g <strong>in</strong> B. diffusa functions as a signal molecule and is ofgreat <strong>in</strong>terest as it has a role <strong>in</strong> stimulat<strong>in</strong>g the defence system of <strong>plant</strong>saga<strong>in</strong>st viruses (S<strong>in</strong>gh, 2006; Verma et al., 2006). The VIA is present <strong>in</strong> bothtreated and untreated leaves (Verma and Awasthi, 1980). Micropropagationof B. diffusa has been carried out for produc<strong>in</strong>g the systemic RIP for viraldisease <strong>management</strong> (Gupta, 1999; Gupta et al., 2004).Susceptible healthy hosts upon treatment with C. aculeatum extractdevelop complete resistance with<strong>in</strong> 4–6 h. The SIR is reversed by the simultaneousapplication of AMD (Verma et al., 1984). Treatment with SRI from C.aculeatum leaves, triggers accumulation of a new defensive VIA <strong>in</strong> treatedand non-treated leaves of healthy host <strong>plant</strong>s (Verma et al., 1984; Verma et al.,1996, 1999; Srivastava 1999). The SRI <strong>in</strong> crude sap is resistant to denaturationby organic solvents and extremely thermostable (Verma et al., 1984). Theresistance-<strong>in</strong>duc<strong>in</strong>g activity of C. aculeatum SRI (CA-SRI) is not affected byexogenous application of proteases (Verma et al., 1996). Leaf extract of C. aculeatumis most effective for controll<strong>in</strong>g virus diseases <strong>in</strong> crop <strong>plant</strong>s (Vermaet al., 1995a; Srivastava 1999; Srivastava et al., 2004). Leaf extract, whensprayed on susceptible host <strong>plant</strong>s, prevents <strong>in</strong>fection of mechanically andwhite fly transmitted viruses <strong>in</strong> several hosts, e.g. tomato yellow mosaicvirus, tobacco mosaic virus, sunnhemp rosette virus and tobacco leaf curlvirus (Verma et al., 1984; Verma et al., 1995a) (Table 7.2).Table 7.2. Plants conta<strong>in</strong><strong>in</strong>g virus <strong>in</strong>hibitory activity.Name Family ReferenceAmaranthus albus Amaranthaceae Smookler, 1971Alternanthera brasiliana Amaranthaceae Noronha et al., 1983Acacia arabicaLegum<strong>in</strong>osaeGupta and Raychauduri, 1971a(Fabaceae)Acer <strong>in</strong>sulera Aceraceae Yoshi et al., 1954Agava americana Agavaceae Simon, 1963Ailanthes excelsa Simaroubaceae Patel and Patel, 1979Alternanthere fi coidea Amaranthaceae Noronha et al., 1983Amaranthus caudatus Amaranthaceae Smooker, 1971Argemone mexicana Papaveraceae Patel and Patel, 1979Beta vulgaris Chenopodiaceae Paliwal and Nar<strong>in</strong>ai, 1965Cont<strong>in</strong>ued

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