natural-products-in-plant-pest-management

100 K.A. RaveeshaConcentration of the bioactivecompound (ppm)10009008007006005004003002001000Aspergillus flavusA. nigerA. tamariiA. fumigatusA. ochraceousA. flavipesFungiA. terreusA. flavus oryzaeA. candidusA. flavus columnarisPenicillium notatumP. chrysogenumFig. 4.5. Minimal inhibitory concentration (MIC) of the bioactive compound [2H-furo[2,3-H]-1-benzopyran-2-one] isolated from seeds of P. corylifolia L. against species of Aspergillusand Penicillium.A. tamarii, A. terreus and A. candidus was not observed even at 1000 ppm.Penicillium notatum and P. chrysogenum were totally inhibited at 800 ppm and900 ppm concentration, respectively.The concentration of the bioactive compound needed for total inhibitionof Aspergillus and Penicillium species was much lower than the recommendeddose of the test fungicides.In vivo evaluation of the active component to prevent the biodeterioration ofmaizeMaize grains naturally infected with diverse species of Aspergillus and Penicilliumwere treated with 250, 500, 1000 and 1500 ppm concentration of thebioactive compound. Untreated seeds served as a control. The treated anduntreated seeds were stored at room temperature (30 ± 2°C) for 4 months andthe moisture content of the seed sample was maintained at 13.5% ( Janar dhanaet al., 1999). Samples were drawn at regular intervals of 30, 60, 90 and 120days from each treatment and subjected to seed mycoflora analysis employingthe standard method (ISTA, 1999), determination of protein content(Lowry et al., 1951) and carbohydrate content (Dubios et al., 1956) to assessthe level of biodeterioration.Results revealed a significant reduction in the seed mycoflora in all thetreatments with a total elimination of seed-borne fungi including species ofAspergillus and Penicillium in the grains treated with 1000 ppm and 1500 ppmconcentration of the bioactive compound in all the storage periods tested. No