Colletotrichum: complex species or species ... - CBS - KNAW

More documents

Recommendations

Info

Weir et al.gossypii var. cephalosporioides are ITS2 and the D2 region ofthe rDNA LSU, neither of which resolves their relationships withinthe C. gloeosporioides complex. Whether the seedling pathogenregarded by Silva-Mann et al. (2005) and Bailey et al. (1996) to beC. gossypii represents the species first described from cotton inthe USA is not known. The genetic relationship of these apparentlybiologically specialised fungi requires additional sequences to begenerated from authentic isolates with known pathogenicity.Colletotrichum gossypii var. cephalosporioides A.S.Costa, Bragantia 6: 5. 1946.≡ Colletotrichum gloeosporioides “var. cephalosporioides” (A.S. Costa)Follin & Mangano, Coton et fibres tropicales 37: 209. 1983. [comb. inval.,no full reference to basionym]Notes: See notes under Colletotrichum gossypii.* Colletotrichum horii B. Weir & P.R. Johnst., Mycotaxon111: 21. 2010.Weir & Johnston (2010) and Xie et al. (2010a) provide descriptions.Geographic distribution and host range: Associated with fruit andstem disease of Diospyros kaki from China, Japan, and NewZealand. Xie et al. (2010a) noted minor symptoms on inoculatedfruit of Capsicum annuum, Musa acuminata, and Cucurbita pepo,but noted that the fungus had never been associated with diseasesymptoms on these hosts from the field.Genetic identification: ITS distinguishes C. horii from all otherspecies.Specimens examined: See Weir & Johnston (2010).Colletotrichum hymenocallidis Yan L. Yang, Zuo Y. Liu,K.D. Hyde & L. Cai, Fungal Diversity 39: 138. 2009.Notes: Placed here in synonymy with Colletotrichum siamense.See notes and additional specimens examined under C. siamense.Yang et al. (2009) reported this species as a leaf pathogen ofHymenocallis americana. They distinguished C. hymenocallidisfrom C. siamense, also described from Hymenocallis, primarilyon the basis of a multi-gene phylogeny and differences in colonycolour. Although gene selection was appropriate for resolvinggenetic relationships within the C. gloeosporioides group, Yanget al. (2009) included only five isolates of the C. gloeosporioidescomplex in their phylogeny. Based on this isolate selection, theC. hymenocallidis isolates were genetically distinct from theC. siamense isolates. However, in our analysis, in which the C.siamense/C. hymenocallidis group is represented by 30 isolatesfrom a wide range of hosts from all over the world, authentic isolatesof the two species fall within a monophyletic clade that cannot befurther subdivided phylogenetically.The Latin part of the C. hymenocallidis protologue designatesa culture (“Holotypus: Cultura (CSSN2)”) as the holotype butthe English citation of the type specimen corrects this apparentmistake, citing CSSN2 as an ex-holotype culture, with the herbariumspecimen GZAAS 080001 as the holotype.Specimen examined: China, Guangxi, Nanning, on Hymenocallis americana leafspot, coll. Y.L. Yang GZAAS 080001, 19 Jun 2008 (ex-holotype culture of C.hymenocallidis − CBS 125378 = ICMP 18642).Colletotrichum ignotum E.I Rojas, S.A. Rehner & Samuels,Mycologia 102: 1331. 2010.Notes: Placed here in synonymy with Colletotrichum fructicola. Seenotes and additional specimens examined under C. fructicola.Specimen examined: Panama: Barro Colorado Monument, Tetragastris panamensisleaf endophyte, coll. E.I. Rojas E886, 1 Jun 2004 (ex-holotype culture of C.ignotum − CBS 125397 = ICMP 18646).Colletotrichum jasmini-sambac Wikee, K.D. Hyde, L. Cai& McKenzie, Fungal Diversity 46: 174. 2011.Notes: Placed here in synonymy with Colletotrichum siamensebased on the ITS, GAPDH, CAL, TUB2, and ACT gene sequencesfrom the ex-holotype culture, deposited in GenBank by Wikee etal. (2011).Wikee et al. (2011) discussed similarities between C. jasminisambac,C. siamense and C. hymenocallidis, three speciesgenetically close in their phylogenetic analysis. The broader rangeof isolates representing C. siamense in our analysis shows thatthese species form a single, monophyletic clade that cannot besensibly subdivided (see notes under C. siamense).Specimen examined: Vietnam, Cu Chi District, Trung An Ward, on living leaves ofJasminum sambac, Jan. 2009, coll. Hoa Nguyen Thi LLTA–01 (ex-holotype cultureof C. jasmini-sambac – CBS 130420 = ICMP 19118).* Colletotrichum kahawae J.M. Waller & Bridge subsp.kahawae, Mycol. Res. 97: 993. 1993. Fig. 25.Waller et al. (1993) provide a description.Geographic distribution and host range: Known only from Coffeafrom Africa.Genetic identification: ACT, CAL, CHS-1, GAPDH, TUB2, SOD2,and ITS sequences are the same as those from C. kahawaesubsp. ciggaro. The two subspecies can be distinguished by GSsequences; C. kahawae subsp. kahawae has a 22 bp deletion anda single C to T transition. Collectively, the two subspecies can bedistinguished from all other species using ITS sequences alone.Notes: Colletotrichum kahawae was proposed by Waller et al. (1993)as a name to refer specifically to Colletotrichum isolates causingCoffee Berry Disease (CBD), to taxonomically distinguish thesedisease-causing isolates from the several other Colletotrichumspp. that can be isolated from coffee plants, including C. coffeanum(see notes under C. coffeanum). Colletotrichum kahawae is anapparently clonal population (Varzea et al. 2002), widespread oncoffee in Africa, and with a distinctive growth form and biology(Waller et al. 1993).In this paper C. kahawae sensu Waller et al. (1993) is reducedto subspecies. Based on ACT, CAL, CHS-1, GAPDH, TUB2, SOD2,and ITS gene sequences the coffee berry pathogen cannot bedistinguished from isolates from a wide range of other hosts thatare not pathogenic to coffee. Those other isolates are referred tohere as C. kahawae subsp. ciggaro. We retain a distinct taxonomiclabel for the coffee berry pathogen to reflect its biosecurityimportance. In addition to its biology, C. kahawae subsp. kahawaecan be distinguished metabolically, and genetically using GS genesequences. Waller et al. (1993) used a metabolic test, an inability156
The Colletotrichum gloeosporioides species complexFig. 25. Colletotrichum kahawae subsp. kahawae. A, E. ICMP 17905 (ex IMI 361501). B–C. ICMP 17816 (ex IMI 319418 – ex-holotype culture). C. ICMP 17915 (ex CBS982.69). A–B. Appressoria. C. Conidia. D–E. Cultures on PDA, 10 d growth from single conidia, from above and below. Scale bar C = 20 µm. Scale bar of C applies to A–C.www.studiesinmycology.org157
Page 1:
Studies in Mycology 73 (September 2
Page 5 and 6:
Colletotrichum: complex species or
Page 10 and 11:
Damm et al.Table 1. Strains of Coll
Page 12 and 13:
Damm et al.Table 1. (Continued).Gen
Page 14 and 15:
Damm et al.combined sequence datase
Page 16 and 17:
Damm et al.Fig. 2. Colletotrichum a
Page 19 and 20:
The Colletotrichum boninense specie
Page 21 and 22:
Page 23 and 24:
Page 25 and 26:
Page 27 and 28:
Page 29 and 30:
Page 31 and 32:
Page 33 and 34:
Page 35 and 36:
Page 37 and 38:
Page 39 and 40:
Page 41 and 42:
Page 43 and 44:
Page 45 and 46:
available online at www.studiesinmy
Page 47 and 48:
The Colletotrichum acutatum species
Page 49 and 50:
Page 51 and 52:
Page 53 and 54:
Page 55 and 56:
Page 57 and 58:
Page 59 and 60:
Page 61 and 62:
Page 63 and 64:
Page 65 and 66:
Page 67 and 68:
Page 69 and 70:
Page 71 and 72:
Page 73 and 74:
Page 75 and 76:
Page 77 and 78:
Page 79 and 80:
Page 81 and 82:
Page 83 and 84:
Page 85 and 86:
Page 87 and 88:
Page 89 and 90:
Page 91:
Page 94 and 95:
Damm et al.µm diam. Setae not obse
Page 96 and 97:
Damm et al.Table 2. Conidia measure
Page 98 and 99:
Damm et al.Table 2. (Continued).Spe
Page 100 and 101:
Damm et al.Table 3. Appressoria mea
Page 102 and 103:
Damm et al.Fig. 25. Colletotrichum
Page 104 and 105:
Page 106 and 107:
Page 108 and 109:
Page 110 and 111:
Page 112 and 113:
Page 114 and 115: Damm et al.to olivaceous grey, filt
Page 116 and 117: Damm et al.are formed directly from
Page 118 and 119: Damm et al.Arx JA von (1957). Die A
Page 120 and 121: Damm et al.Masaba DM, Waller JM (19
Page 122 and 123: 114
Page 124 and 125: Weir et al.grass-inhabiting species
Page 126 and 127: Weir et al.Table 1. A list of strai
Page 128 and 129: Weir et al.Table 1. (Continued).Spe
Page 130 and 131: Weir et al.Table 1. (Continued).Spe
Page 132 and 133: Weir et al.Table 2. Primers used in
Page 134 and 135: Weir et al.11ICMP 17789 Malus USA1I
Page 136 and 137: Weir et al.Kahawae cladeC. alataeC.
Page 138 and 139: Weir et al.A0.991ICMP 17905 Coffea
Page 140 and 141: Weir et al.Conidial width variation
Page 142 and 143: Weir et al.G. c.“f.sp. camelliae
Page 144 and 145: Weir et al.Fig. 10. Colletotrichum
Page 154 and 155: Weir et al.Fig. 18. Glomerella cing
Page 156 and 157: Weir et al.images under C. fructico
Page 158 and 159: Weir et al.Specimen examined: Thail
Page 162 and 163: Weir et al.Notes: First described f
Page 166 and 167: Weir et al.to utilise either citrat
Page 170 and 171: Weir et al.(ex-epitype culture CBS
Page 172 and 173: Weir et al.of this species to C. ps
Page 184 and 185: Weir et al.Table 4. Performance of
Page 186 and 187: Weir et al.Crous PW, Gams W, Stalpe
Page 188 and 189: Weir et al.Simmonds JH (1968). Type
Page 190 and 191: Cannon et al.182
Page 192 and 193: Cannon et al.In rare instances, Col
Page 194 and 195: Cannon et al.Table 1. Summary of pr
Page 196 and 197: Cannon et al.Table 1. (Continued).P
Page 198 and 199: Cannon et al.Table 2. Colletotrichu
Page 200 and 201: Cannon et al.as did those for Colle
Page 202 and 203: Cannon et al.Table 3. Authentic seq
Page 204 and 205: Cannon et al.Table 3. (Continued).S
Page 210 and 211: Cannon et al.110.74 C. cuscutae IMI
Page 212 and 213: Cannon et al.Boninense cladeThe bon
Page 214 and 215:
Cannon et al.C. lindemuthianum AB08
Page 216 and 217:
Cannon et al.A further important ac
Page 218 and 219:
Cannon et al.Hawksworth DL (2011).
Page 220 and 221:
Cannon et al.Rodríguez-Guerra R, R
Page 223 and 224:
Studies in MycologyStudies in Mycol
Page 225 and 226:
CBS Biodiversity SeriesNo. 11:Taxon
Page 227:
Studies in Mycology (ISSN 0166-0616
show all

Colletotrichum: complex species or species ... - CBS - KNAW

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?