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Weir et al.of this species to C. psidii remains unknown. A new species onPsidium guajava, C. guajavae, belonging to the C. acutatumspecies complex, is described elsewhere in this volume (Dammet al. 2012a).Specimen examined: Italy, Rome, on Psidium sp., coll. M. Curzi (authentic cultureof C. psidii – CBS 145.29 = ICMP 19120).Glomerella psidii (Delacr.) J. Sheld., Bull. West VirginiaAgric. Exp. Sta. 104: 311. 1906.Basionym: Gloeosporium psidii Delacr., Bull. Soc. Mycol. France.19: 144. 1903.Notes: Sheldon (1906) produced perithecia in culture fromisolates he considered typical of Gloeosporium psidii and on thisbasis recombined the species described by Delacroix (1903) inGlomerella. The relationship of G. psidii to Colletotrichum psidii,also described from guava, is not known. See notes under C. psidii.* Colletotrichum queenslandicum B. Weir & P.R. Johnst.,nom. nov. et stat. nov. MycoBank MB563593. Fig. 31.Basionym: Colletotrichum gloeosporioides var. minus Simmonds,Queensland J. Agric. Anim. Sci. 25: 178A. 1968. [as var. minor]Etymology: based on the region from which the type specimen ofthis species was collected.Holotype: Australia, Queensland, Ormiston, on Carica papaya, coll.J.H. Simmonds, Oct. 1965, IMI 117612.Epitype: Australia, Queensland, Brisbane, on Carica papaya, coll.J.H. Simmonds 11663C, Sep. 1965, epitype here designated PDD28797; ex-epitype culture ICMP 1778.Colonies grown from single conidia on Difco PDA 62–74 mm diamafter 10 d, aerial mycelium either dense, cottony, uniform, grey,or with aerial mycelium lacking, towards centre of colony withnumerous, small acervuli with dark bases and orange conidialooze; in reverse cultures with copious aerial mycelium uniformlydark grey (1F2), those with little aerial mycelium having a pinkishbrown (8B4) pigment within the agar, the dark bases of the acervuliand the colour of the conidial ooze visible through the agar. Conidia(12–)14.5–16.5(–21.5) × (3.5–)4.5–5(–6) µm (av. 15.5 × 4.8 µm, n= 96), cylindric, straight, sometimes slightly constricted near centre,ends broadly rounded. Appressoria about 6–12 µm diam., globoseto short-cylindric, rarely lobed. Perithecia not seen.Geographic distribution and host range: Known from Carica papayaand Persea americana from Queensland, Australia, and fromCoffea berries from Fiji. Simmonds (1965) reported from Australiawhat he considered to be the same fungus also from Mangiferaindica, Malus sylvestris, and “many other hosts”.Genetic identification: ITS sequences do not separate C.queenslandicum from some C. fructicola, some C. siamense, andsome C. tropicale isolates. It is best distinguished from these taxausing TUB2, GAPDH, or GS.Notes: The ex-type cultures cited by Simmonds (1968) are nolonger in storage at BRIP in Queensland (R. Shivas, pers. comm.)and presumably lost. However, we do have two cultures identifiedas C. gloeosporioides var. minus by Simmonds and isolated fromthe same host from the same locality as the holotype (Simmondsisolates 16633C and 1647A2), that had been sent to Joan Dingleyin 1965 and subsequently stored in the ICMP culture collection.The culture selected here as epitype (Simmonds 11663C = ICMP1778) matches the Simmonds (1965) description of this fungus ashaving “an abundance of aerial mycelium in culture”. Our conidialmeasurements from ICMP 1778 and 1780 are broader than thosegiven by Simmonds (1965), but he does note that “Confusion canoccur between narrower strains of C. gloeosporioides and broaderstrains of C. gloeosporioides var. minus …”. Simmonds (1965) alsonotes that perithecia may rarely be seen in cultures of some isolates.The isolates accepted here as C. queenslandicum aregenetically distinct within the Musae clade of C. gloeosporioides s.lat. Colletotrichum minus Zimm. (1901) requires that we propose anom. nov. for this fungus at species rank.Simmonds (1965) considered C. gloeosporioides var. minusto be the conidial state of Glomerella cingulata var. minorWollenw. Wollenweber & Hochapfel (1949) used the nameGloeosporium elasticae Cooke & Massee for the conidial stateof G. cingulata var. minor, the type specimens for both namesbeing from Ficus. Simmonds (1965) noted that it was not possibleto transfer G. elasticae to Colletotrichum because Colletotrichumelasticae had already been published for a different fungus.However, rather than proposing a nom. nov. for Gloeosporiumelasticae, he described C. gloeosporioides var. minus as a newvariety, with a different type specimen. Glomerella cingulata var.minor is genetically distinct from the specimen Simmonds choseas the type of C. gloeosporioides var. minus, see notes under G.cingulata var. minor.Other specimens examined: Australia, Queensland, Brisbane, on Carica sp.,coll. J.H. Simmonds 16347A2 (ICMP 1780, dried culture stored as PDD 28797);Queensland, Home Hill, on Persea americana, coll. L. Coates 22516, Feb. 1983(ICMP 12564). Fiji, on Coffea sp. berry, coll. R. Gounder, Apr. 1988 (ICMP 18705).Glomerella rufomaculans var. vaccinii Shear, Bull. TorreyBot. Club. 34: 314. 1907.Notes: Placed here in synonymy with Colletotrichum kahawaesubsp. ciggaro. See notes under C. kahawae subsp. ciggaro. Notethat Saccardo & Trotter (1913) place Shear’s variety in Glomerellafructigena (Clint.) Sacc., a rarely used species name, placed insynonymy with G. cingulata by von Arx & Müller (1954).Specimen examined: USA, on Vaccinium macrocarpum leaves, coll. C.L. Shear,Apr. 1922 (authentic isolate of G. rufomaculans var. vaccinii – CBS 124.22 = ICMP19122).* Colletotrichum salsolae B. Weir & P.R. Johnst., sp. nov.MycoBank MB563589. Fig. 32.= Colletotrichum gloeosporioides “f. sp. salsolae” (Berner et al. 2009).Etymology: Based on C. gloeosporioides “f. sp. salsolae”, referringto the host from which this fungus was originally collected.Holotype: Hungary, on Salsola tragus, coll. D. Berner [specimenfrom plants inoculated with strain 96-067, originally collected I.Schwarczinger & L. Vajna on Salsola tragus from Bugac, nearKiskunsag National Park, 1996], BPI 878740; ex-holotype cultureICMP 19051.Colonies grown from single conidia on Difco PDA 38–42 mm diamafter 10 d, aerial mycelium sparse, cottony, pale grey, surface of164
The Colletotrichum gloeosporioides species complexFig. 31. Colletotrichum queenslandicum. A, C, E. ICMP 1778 – ex-epitype culture. B, F. ICMP 1780. D, G. ICMP 12564. H. ICMP 18705. A–B. Appressoria. C–D. Conidia. E–H.Cultures on PDA, 10 d growth from single conidia, from above and below. Scale bar A = 20 µm. Scale bar of A applies to A–D.colony dark, a more or less continuous layer of acervulus-likestructure with deep orange brown conidial masses and numeroussetae; in reverse dark purplish-black near centre of colony, darkolivaceous near the margin. Conidia (10–)14–16.5(–20.5) × (4.5–)5.5–6(–7.5) µm (av. 15.3 × 5.8 µm, n = 24), highly variable in sizeand shape, subglobose to long-cylindric, apex usually broadlywww.studiesinmycology.org165
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