ISQ User Guide - Write Frame of Mind

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7 Optimizing Your Method Modifying an Automatic Tune – Custom—Select this option if you do not want to use the default value for the tune. For the electron lens negative voltage, the default is -75 V. You should tune with the same value you are planning to use for your analysis. This value affects how well the electrons are kept from entering the ion source when they are not supposed to. If you change your electron energy from 70 V, this value will also change. This voltage should always be at least 5V below the voltage applied to the filament. The voltage applied to the filament is the same number, but the opposite sign, of the electron energy. If you set this value to be smaller than the electron energy, for example, if the EE is 70 eV, and the negative voltage is set to -50 V, then electrons can not be stopped from entering the ionization region. If the negative voltage is set to -75 V, though, the electrons will be blocked from entering the source. This field is not used at this time. • Set Ion Source Temperature—Select this checkbox to enable the temperature field. Then enter a value between 0 and 350 °C (default is 200 °C). The optimal temperature is determined by the molecular structure and weight of the compounds you are analyzing. Heavier compounds require a higher temperature. You should set the temperature as high as possible to keep the ion source clean and maintain the right amount of sensitivity. 8. Configure the Lenses tab. The lens tune is the main portion of the tune algorithm. In this section you can choose which components to tune, which mass to optimize for the tune, what the range of allowed values are, how to move through the range, how much the signal must change for a new value to be selected, and what (if anything) should be done about the resolution of the peak and any errors that occur. • Device—Use this pull-down menu to select the component you want to tune according to the settings in the other columns. 82 ISQ User Guide Thermo Scientific
7 Optimizing Your Method Modifying an Automatic Tune – Repeller—Select this option to control how the repeller pushes the ions out of the ionization region. The voltage applied to this component will have a very strong effect on the energy of the ion beam, which will have a strong effect on the resolution and the intensity. The lower the voltage, the better the resolution. However, higher voltages will prevent ions from striking the repeller surface, which leads to better robustness. Typical values are 0 to 5 V, although a dirty system may have the repeller climb as high as 12.5 V. We do not recommend setting the repeller any higher. – Lens 1—Select this option to control Lens 1, which is the first of three lenses that the ions see as they leave the ionization region. These three lenses act as a focusing element to maximize the ion beam intensity that is entering the ion guide. This field is typically set between -35 and -50 V. – Lens 2—Select this option to control Lens 2, which is the second of three lenses that the ions see as they leave the ionization region. These three lenses act as a focusing element to maximize the ion beam intensity that is entering the ion guide. This field is typically set between 0 and -15 V in EI mode. In CI, the optimal voltage may be between 0 and -30 V. – Lens 3—Select this option to control Lens 3, which is the last of three lenses that the ions see as they leave the ionization region. These three lenses act as a focusing element to maximize the ion beam intensity that is entering the ion guide. This field is typically set near the same voltage as lens 1. – Ion Guide DC—Select this option to control the ion guide’s DC offset voltage. It can potentially help focus the ions into the quadrupole while ensuring that neutral noise is eliminated. The voltage on this component is mass dependant and should be set at several different masses. This field is typically set between +1 and -15 V, depending on the mass of the ion. – Ion Guide RF—Select this option to control the ion guide’s RF voltage. It can potentially help focus the ions into the quadrupole while ensuring that neutral noise is eliminated. The voltage on this component is mass dependant and should be set at several different masses. This field is typically set between 0 and +5 V, depending on the mass of the ion. – Q1—Select this option to control the voltage that pulls the ions into the quadrupole. The voltage applied to this component will have a very strong effect on the energy of the ion beam, which will have a strong effect on the resolution and the intensity. The lower the voltage, the better the resolution. However, higher voltages will pull more ions into the quads, which leads to better signal. This field is typically set between 0 and -5 V. – Resolution—Select this option to adjust the ratio of the quadrupole DC and RF voltages to create the resolution required for your analysis. You can set the desired peak width at a given mass and whether you measure the width at 10% or 50% of the peak height. Because there is no static DC voltage involved, the start, stop, and step values are not used. • Mass—Use this pull-down menu to select the ion to be used for tuning. Thermo Scientific ISQ User Guide 83
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ISQ User Guide 1R120555-0003 Revisi
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Manufacturer: Thermo Fisher Scienti
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WEEE Compliance This product is req
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Contents Thermo Scientific ISQ User
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Preface About Your System This guid
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Related Documentation Preface Relat
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Hydrogen Safety Precautions Preface
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Hydrogen Connection Guidelines Use
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Properly Storing Hydrogen Preface H
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Preface Hydrogen Safety Precautions
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Changing the Column The ISQ ships w
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Replacing the Factory-Installed Col
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15. Perform a column leak check. 1
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j. Repeat the leak check until no l
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i. The following message will indic
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1 Changing the Column Replacing the
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Confirming Your GC/MS System is Wor
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Checking the Vacuum and Temperature
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Tuning the ISQ Tuning will improve
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3 Tuning the ISQ Using ISQ Autotune
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3 Tuning the ISQ Using ISQ Autotune
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9. Click the Print Report button to
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3 Tuning the ISQ Tuning the ISQ wit
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Page 51 and 52: Creating a Method Once you’ve got
Page 53 and 54: . Configure the Pre-Injection group
Page 55 and 56: 4 Creating a Method b. Set the MS T
Page 57 and 58: 4 Creating a Method At 4 minutes in
Page 59 and 60: 4 Creating a Method • The Filamen
Page 61 and 62: 4 Creating a Method You can select
Page 63 and 64: 4 Creating a Method Finally, if you
Page 65 and 66: If you have a FOCUS GC: 4 Creating
Page 67 and 68: 4 Creating a Method c. Click the Ca
Page 69 and 70: 4 Creating a Method 6. When you are
Page 71 and 72: Running a Sample Preparing Your Sam
Page 73 and 74: 5 Running a Sample Running a Sequen
Page 83 and 84: e. Configure the Run Synchronously
Page 85 and 86: Exploring Your Data Once you have a
Page 87 and 88: 6 Exploring Your Data 5. The chroma
Page 89 and 90: 6 Exploring Your Data 8. Right-clic
Page 91 and 92: 6 Exploring Your Data 12. In this s
Page 93 and 94: 6 Exploring Your Data The full scan
Page 95 and 96: Optimizing Your Method If you did n
Page 97 and 98: Modifying an Automatic Tune 7 Optim
Page 99 and 100: 6. Configure the General tab. • N
Page 101: 7 Optimizing Your Method Modifying
Page 105 and 106: 7 Optimizing Your Method Modifying
Page 107 and 108: 7 Optimizing Your Method Modifying
Page 109 and 110: Troubleshooting This section contai
Page 111 and 112: Investigating Baseline Issues Table
Page 113 and 114: Table 3. Troubleshooting Peak Issue
Page 115 and 116: Table 3. Troubleshooting Peak Issue
Page 117 and 118: Table 4. Troubleshooting Results Is
Page 119 and 120: 8 Troubleshooting Reconfiguring You
Page 125 and 126: 16. Click the Done button to close
Page 127 and 128: Index A acquisition methods, purpos
Page 129 and 130: tune report, 22, 29 tune types addi
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