ISQ User Guide - Write Frame of Mind

4 Creating a Method
f. In the Run Completion group, select the action you want to occur at the end of a
run:
• GC Run Time—Select this option if you want the ISQ run to end when the GC
run is complete. This is the most common setting.
• Probe Run Time—Select this option if you have a probe controller installed and
you want the ISQ run to end when the probe run is complete.
• Select Stop After—Select this option to set the number of minutes you want the
ISQ to run. The end of the run can be between 0 and 1,000 minutes. This
option allows you to stop the acquisition when all the compounds of interest
have eluted, but the GC is still at an elevated temperature to keep the column
clean. We recommend you select this option it saves burn time on the filament.
g. In the Scans pane, click a scan row to enter scan information.
Note If some of the columns mentioned below are not shown in the Method Setup pane,
you can right-click on a heading and display them. You can also reorganize the columns by
clicking on the heading of a column and dragging it to the left or right.
• The Time (min) column is used to set the time that the ISQ begins to acquire
data after the GC starts. It is typical to have enough of a time delay to allow the
solvent to get through the column before starting an acquisition.
As an example, in this method, the mass range of 50-550 amu will be scanned in
0.2 seconds. At the same time, three different SIM masses at 100, 150, and 200
will be looked at for 0.2 seconds each. These simultaneous full scan and SIM
scans will begin 2.5 minutes into the GC run. You will get a complete set of scans
every 0.816 seconds.
36 ISQ User Guide Thermo Scientific