ISQ User Guide - Write Frame of Mind

8 Troubleshooting
Investigating Results Issues
Investigating Results Issues
Table 4. Troubleshooting Results Issues in Your Data
Behavior Cause Characteristic Remedy
Low
Reproducibility of
Peaks Area
General
Detector gain is set too low
Concentration is not compatible
with the dynamic range of the
detection system
Chromatogram and spectrum are
blank
Injection technique is not
appropriate
Injection parameters are not
appropriate
Sample injection technique is not
reproducible
Syringe or septum is leaking
Injection has leaks
Injection technique is not suitable
Split flow or ratio control is
inadequate
Retune the gain.
Increase the electron multiplier voltage.
Increase the target ion count.
Verify that the sample concentration is suitable for
the ISQ.
Make sure the tune file is correct.
Verify that the Busy light is on during acquisition.
Make sure the filament is not burned out.
Use a different injection technique.
Verify the injection temperature and flow rates.
Evaluate the sample preparation sequences.
Compare the results with a series of standard
injections.
Check and replace the syringe and/or septum at
regular intervals.
Check the column connections.
Run a leak check.
Carefully meter the injected amount.
Use a clean, good-quality syringe.
Monitor the flow.
Replace the in-line filter.
96 ISQ User Guide Thermo Scientific