ISQ User Guide - Write Frame of Mind

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7 Optimizing Your Method Improving the Way You Prepare Samples Improving the Way You Prepare Samples Changing the Scan Rate Narrowing the Mass Range Although sample preparation adds time and expense to the overall analysis, a more focused method can give you better results. Try extracting your sample in a solvent that increases the solubility of the analytes of interest, but does not increase the solubility of the other compounds. Try switching solvents if your method will allow it. You can also use or change the phase of a solid phase extraction cartridge, which gives you similar results as changing a solvent. You can affect the way you prepare samples by changing the type of cartridge you are using. The precision of your data depends on how well you define your chromatographic peak. Typically, you get good precision when sampling ten times across the chromatographic peak. Increasing the scan rate increases the number of times you’ve sampled across the peak. However, increasing the scan rate too much results in mass spectral noise, which decreases your analytical precision. To optimize your scan rate, select a rate that will give you 8-12 points across a chromatographic peak. By narrowing your mass range, you can look directly at the compounds of interest. However, if you’re looking at a large number of compounds that have a broad range of mass fragments, a wide mass range makes sense. To narrow the mass range, refine your scan parameters to a smaller number. A narrower mass range will also allow you to decrease the scan rate and get the same chromatographic peak sampling. Adjusting the Transfer Line Temperature If your transfer line temperature is set too low, the less volatile compounds may get stuck in the transfer line and never make it into the ion source. On the other hand, if your transfer line is too hot, you could damage the column or cause a thermal breakdown in the compounds you are analyzing. Typically, the transfer line temperature should be 10 °C over the highest boiling point of the compounds of interest, but no more than the maximum safe operating temperature of the column. 76 ISQ User Guide Thermo Scientific
Modifying an Automatic Tune 7 Optimizing Your Method Modifying an Automatic Tune ISQ Autotune is a utility that uses certain parameters in the tune types to optimize system performance when generating a tune file. To modify an automatic tune: 1. Double-click the Xcalibur software icon on your computer desktop. 2. In the Xcalibur Roadmap window, click the Instrument Setup icon. Thermo Scientific ISQ User Guide 77
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ISQ User Guide 1R120555-0003 Revisi
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Manufacturer: Thermo Fisher Scienti
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WEEE Compliance This product is req
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Contents Thermo Scientific ISQ User
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Preface About Your System This guid
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Related Documentation Preface Relat
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Hydrogen Safety Precautions Preface
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Hydrogen Connection Guidelines Use
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Properly Storing Hydrogen Preface H
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Preface Hydrogen Safety Precautions
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Changing the Column The ISQ ships w
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Replacing the Factory-Installed Col
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15. Perform a column leak check. 1
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j. Repeat the leak check until no l
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i. The following message will indic
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1 Changing the Column Replacing the
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Confirming Your GC/MS System is Wor
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Checking the Vacuum and Temperature
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Tuning the ISQ Tuning will improve
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3 Tuning the ISQ Using ISQ Autotune
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3 Tuning the ISQ Using ISQ Autotune
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9. Click the Print Report button to
Page 45 and 46: 3 Tuning the ISQ Tuning the ISQ wit
Page 51 and 52: Creating a Method Once you’ve got
Page 53 and 54: . Configure the Pre-Injection group
Page 55 and 56: 4 Creating a Method b. Set the MS T
Page 57 and 58: 4 Creating a Method At 4 minutes in
Page 59 and 60: 4 Creating a Method • The Filamen
Page 61 and 62: 4 Creating a Method You can select
Page 63 and 64: 4 Creating a Method Finally, if you
Page 65 and 66: If you have a FOCUS GC: 4 Creating
Page 67 and 68: 4 Creating a Method c. Click the Ca
Page 69 and 70: 4 Creating a Method 6. When you are
Page 71 and 72: Running a Sample Preparing Your Sam
Page 73 and 74: 5 Running a Sample Running a Sequen
Page 83 and 84: e. Configure the Run Synchronously
Page 85 and 86: Exploring Your Data Once you have a
Page 87 and 88: 6 Exploring Your Data 5. The chroma
Page 89 and 90: 6 Exploring Your Data 8. Right-clic
Page 91 and 92: 6 Exploring Your Data 12. In this s
Page 93 and 94: 6 Exploring Your Data The full scan
Page 95: Optimizing Your Method If you did n
Page 99 and 100: 6. Configure the General tab. • N
Page 101 and 102: 7 Optimizing Your Method Modifying
Page 109 and 110: Troubleshooting This section contai
Page 111 and 112: Investigating Baseline Issues Table
Page 113 and 114: Table 3. Troubleshooting Peak Issue
Page 115 and 116: Table 3. Troubleshooting Peak Issue
Page 117 and 118: Table 4. Troubleshooting Results Is
Page 119 and 120: 8 Troubleshooting Reconfiguring You
Page 125 and 126: 16. Click the Done button to close
Page 127 and 128: Index A acquisition methods, purpos
Page 129 and 130: tune report, 22, 29 tune types addi
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