ISQ User Guide - Write Frame of Mind

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Contents Changing the Scan Rate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76 Narrowing the Mass Range . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76 Adjusting the Transfer Line Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76 Modifying an Automatic Tune . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77 Chapter 8 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .89 How to Know When Your System Needs Maintenance . . . . . . . . . . . . . . . . . . 90 Investigating Baseline Issues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 Investigating Peak Issues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92 Investigating Results Issues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96 Reconfiguring Your Instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98 viii ISQ User Guide Thermo Scientific
Preface About Your System This guide contains detailed information about how to use the Thermo Scientific ISQ single quadrupole GC-MS system. The ISQ is designed to stay cleaner, longer, to maximize your instrument’s uptime and improve your lab’s productivity. The need to break vacuum, cool off your system, and spend hours cleaning, reassembling, and restoring the system is gone. The heated ion volume, lens stack, and ion optics path in the ISQ ensure that the system stays cleaner longer, but when the system no longer meets your analytical needs, restoring performance is quick and easy. The ExtractaBrite source incorporates a cartridge that contains the repeller, source lenses, and RF lens, all of which can be removed from the instrument while still under vacuum. What once took hours, or even an entire day, is now accomplished in minutes. Thermo Fisher Scientific systems provide the highest caliber gas chromatography/mass spectrometry (GC/MS) instrumentation available on today’s market. GC/MS represents a combination of two powerful analytical techniques: GC, which acts as a separation technique and MS, which acts as a detection technique. Complex mixtures of individual compounds can be injected into the GC, either manually or through the use of an optional autosampler, and then separated for presentation to the MS. The MS will then generate a mass spectrum of the GC eluent and its components, which can be used for qualitative identification, as well as accurate and precise quantification of the individual compounds present in the sample. WARNING Thermo Fisher Scientific systems operate safely and reliably under carefully controlled environmental conditions. If the equipment is used in manner not specified by the manufacturer, the protections provided by the equipment may be impaired. If you maintain a system outside the specifications listed in this guide, failures of many types, including personal injury or death, may occur. The repair of instrument failures caused by operation in a manner not specified by the manufacturer is specifically excluded from the Standard Warranty and service contract coverage. Thermo Scientific ISQ User Guide ix P
Page 1 and 2: ISQ User Guide 1R120555-0003 Revisi
Page 3 and 4: Manufacturer: Thermo Fisher Scienti
Page 5 and 6: WEEE Compliance This product is req
Page 7: Contents Thermo Scientific ISQ User
Page 11 and 12: Related Documentation Preface Relat
Page 13 and 14: Hydrogen Safety Precautions Preface
Page 15 and 16: Hydrogen Connection Guidelines Use
Page 17 and 18: Properly Storing Hydrogen Preface H
Page 19 and 20: Preface Hydrogen Safety Precautions
Page 21 and 22: Changing the Column The ISQ ships w
Page 23 and 24: Replacing the Factory-Installed Col
Page 25 and 26: 15. Perform a column leak check. 1
Page 27 and 28: j. Repeat the leak check until no l
Page 29 and 30: i. The following message will indic
Page 31 and 32: 1 Changing the Column Replacing the
Page 33 and 34: Confirming Your GC/MS System is Wor
Page 35 and 36: Checking the Vacuum and Temperature
Page 37 and 38: Tuning the ISQ Tuning will improve
Page 39 and 40: 3 Tuning the ISQ Using ISQ Autotune
Page 41 and 42: 3 Tuning the ISQ Using ISQ Autotune
Page 43 and 44: 9. Click the Print Report button to
Page 45 and 46: 3 Tuning the ISQ Tuning the ISQ wit
Page 51 and 52: Creating a Method Once you’ve got
Page 53 and 54: . Configure the Pre-Injection group
Page 55 and 56: 4 Creating a Method b. Set the MS T
Page 57 and 58: 4 Creating a Method At 4 minutes in
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4 Creating a Method • The Filamen
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4 Creating a Method You can select
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4 Creating a Method Finally, if you
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If you have a FOCUS GC: 4 Creating
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4 Creating a Method c. Click the Ca
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4 Creating a Method 6. When you are
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Running a Sample Preparing Your Sam
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5 Running a Sample Running a Sequen
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e. Configure the Run Synchronously
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Exploring Your Data Once you have a
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6 Exploring Your Data 5. The chroma
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6 Exploring Your Data 8. Right-clic
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6 Exploring Your Data 12. In this s
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6 Exploring Your Data The full scan
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Optimizing Your Method If you did n
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Modifying an Automatic Tune 7 Optim
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6. Configure the General tab. • N
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7 Optimizing Your Method Modifying
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Troubleshooting This section contai
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Investigating Baseline Issues Table
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Table 3. Troubleshooting Peak Issue
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Table 3. Troubleshooting Peak Issue
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Table 4. Troubleshooting Results Is
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8 Troubleshooting Reconfiguring You
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16. Click the Done button to close
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Index A acquisition methods, purpos
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tune report, 22, 29 tune types addi
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