ISQ User Guide - Write Frame of Mind

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Preface Hydrogen Safety Precautions xx ISQ User Guide Thermo Scientific
Changing the Column The ISQ ships with a factory-tested 15 m x 0.25mm i.d. TR-SQC column, which the Field Service Engineer uses to qualify the instrument. However, once it gets dirty, you cannot purchase a new one, so you need to install a column that accommodates the type and quantity of samples you will be running. You should choose a column that will give you the best possible resolution, analysis speed, and quantitation. When determining the type of column for your particular needs, here are a few things to consider: • Column Material—Columns made out of fused silica are economical and widely used. Columns made out of this material have a wide range of stationary phases and are available in many sizes that can be used with a mass spectrometer. Large diameter columns made of steel are widely used in process gas analysis, but they are not typically used on mass spectrometers. There are also metal-clad, fused silica columns, which have the advantages of fused silica, but the metal makes them resistant to breakage. These columns are less common and more expensive. • Stationary Phase—The stationary phase is the most important consideration when selecting a column. The interaction between the stationary phase and the analyte determines how well the analytes separate from each other (resolution) and also affects how quickly the separation occurs (analysis time). Choose a stationary phase that is compatible with the nature of your analytes and the maximum GC oven temperature you are going to use. • Internal Diameter—The smaller the diameter of the column, the better the separation. However, smaller diameter columns do not have as much capacity for matrix or analytes. As a result, smaller diameter columns are subject to overloading, which leads to retention time shifts and peak shape changes. Larger diameter columns can accept larger concentrations of material, but will require longer columns or slower GC oven temperature ramps (which increases the analysis time) to match the separation power of shorter columns. Typical column sizes for GC/MS have inside diameters (ID) of 0.25 mm. Smaller ID columns, such as the 0.18 and 0.10 mm, are becoming increasingly popular. Additionally, 0.32 and 0.53 mm ID columns are commonly used. Thermo Scientific ISQ User Guide 1 1
Page 1 and 2: ISQ User Guide 1R120555-0003 Revisi
Page 3 and 4: Manufacturer: Thermo Fisher Scienti
Page 5 and 6: WEEE Compliance This product is req
Page 7 and 8: Contents Thermo Scientific ISQ User
Page 9 and 10: Preface About Your System This guid
Page 11 and 12: Related Documentation Preface Relat
Page 13 and 14: Hydrogen Safety Precautions Preface
Page 15 and 16: Hydrogen Connection Guidelines Use
Page 17 and 18: Properly Storing Hydrogen Preface H
Page 19: Preface Hydrogen Safety Precautions
Page 23 and 24: Replacing the Factory-Installed Col
Page 25 and 26: 15. Perform a column leak check. 1
Page 27 and 28: j. Repeat the leak check until no l
Page 29 and 30: i. The following message will indic
Page 31 and 32: 1 Changing the Column Replacing the
Page 33 and 34: Confirming Your GC/MS System is Wor
Page 35 and 36: Checking the Vacuum and Temperature
Page 37 and 38: Tuning the ISQ Tuning will improve
Page 39 and 40: 3 Tuning the ISQ Using ISQ Autotune
Page 41 and 42: 3 Tuning the ISQ Using ISQ Autotune
Page 43 and 44: 9. Click the Print Report button to
Page 45 and 46: 3 Tuning the ISQ Tuning the ISQ wit
Page 51 and 52: Creating a Method Once you’ve got
Page 53 and 54: . Configure the Pre-Injection group
Page 55 and 56: 4 Creating a Method b. Set the MS T
Page 57 and 58: 4 Creating a Method At 4 minutes in
Page 59 and 60: 4 Creating a Method • The Filamen
Page 61 and 62: 4 Creating a Method You can select
Page 63 and 64: 4 Creating a Method Finally, if you
Page 65 and 66: If you have a FOCUS GC: 4 Creating
Page 67 and 68: 4 Creating a Method c. Click the Ca
Page 69 and 70: 4 Creating a Method 6. When you are
Page 71 and 72:
Running a Sample Preparing Your Sam
Page 73 and 74:
5 Running a Sample Running a Sequen
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Page 77 and 78:
Page 79 and 80:
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e. Configure the Run Synchronously
Page 85 and 86:
Exploring Your Data Once you have a
Page 87 and 88:
6 Exploring Your Data 5. The chroma
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6 Exploring Your Data 8. Right-clic
Page 91 and 92:
6 Exploring Your Data 12. In this s
Page 93 and 94:
6 Exploring Your Data The full scan
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Optimizing Your Method If you did n
Page 97 and 98:
Modifying an Automatic Tune 7 Optim
Page 99 and 100:
6. Configure the General tab. • N
Page 101 and 102:
7 Optimizing Your Method Modifying
Page 103 and 104:
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Troubleshooting This section contai
Page 111 and 112:
Investigating Baseline Issues Table
Page 113 and 114:
Table 3. Troubleshooting Peak Issue
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Table 3. Troubleshooting Peak Issue
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Table 4. Troubleshooting Results Is
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8 Troubleshooting Reconfiguring You
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16. Click the Done button to close
Page 127 and 128:
Index A acquisition methods, purpos
Page 129 and 130:
tune report, 22, 29 tune types addi
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