ISQ User Guide - Write Frame of Mind

1 Changing the Column
Replacing the Factory-Installed Column
c. Unwind about one turn of the column from the column outlet end.
Note Wear clean, lint- and powder-free gloves when you handle the column and
transfer line ferrule.
d. Wipe approximately 300 mm (12 in.) of the column with a tissue soaked in
methanol.
e. Insert the column through the transfer line nut, and ferrule. Wipe the column again
with a tissue soaked in methanol.
f. Insert the column into the measuring tool, which is in the ISQ Toolkit, so at least 2
cm extends past the tip.
Tip Slide a septum on the column before adding the transfer line nut to make it easier
to measure the proper distance between the nut and the end of the column. You can
also use a white marker to mark the position of the column and nut.
Figure 5. Using the Column Measuring Tool
g. Tighten the injector nut until the column just barely resists moving in the ferrule.
h. Use a scoring wafer to score and break the column between the grooves (not at the
end). Use a magnifying glass to check for an even, flat cut. Repeat if necessary.
Figure 6. Breaking the Column
i. Mark the nut end of the outlet of the column so you will know how to insert the
column into the ISQ.
j. Remove the column, transfer line nut and ferrule from the column measuring tool.
k. Insert the column into the transfer line. Be careful that the column is inserted to the
proper depth. Use the mark or septa from before to indicate the correct length.
l. Tighten the nut finger-tight plus a quarter turn.
10 ISQ User Guide Thermo Scientific