ISQ User Guide - Write Frame of Mind

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1 Changing the Column Replacing the Factory-Installed Column 7. Cool down the GC inlet and oven. WARNING - BURN HAZARD: The injector, oven, and transfer line may be hot. Allow them to cool to room temperature before touching them. 8. Open the front door of the GC. 9. Unscrew the transfer line nut and remove the column from the transfer line. 10. Unscrew the injector nut and remove the column from the injector. 11. Connect the new column to the injector inside the GC. a. Unwind the column about a half turn. Note Wear clean, lint- and powder-free gloves when you handle the column and injector ferrule. b. Wipe about 100 mm (4 in.) of the column with a tissue soaked in methanol. c. Insert the column through the injector nut and ferrule (open end up). Wipe the column again with a tissue soaked in methanol. d. Use a scoring wafer to score and break the column about 2.5 cm (1 in.) from the end. Use a magnifying glass to check for an even, flat cut. Repeat if necessary. e. Insert the column into the injector so that the end of the column is the proper distance (splitless = 64 mm, split = 40 mm, PTV = 30 mm) from the back of the injector nut. f. Tighten the column nut finger-tight plus a quarter turn. 12. Power-on the GC. 13. Set the carrier gas flow to 1.0 mL/min. 14. Set up the GC. Tip Slide a septum on the column before the injector nut to make it easier to measure the proper distance between the nut and end of the column. a. Set the oven and injector temperatures to 50 °C. b. Turn off vacuum compensation, which is located on the Carrier menu of the GC. c. Use the column flowmeter connector to verify that there is flow through the column. If you do not have a flowmeter, dip the column outlet in a small vial of methanol. Bubbles indicate there is flow through the column. If there is no flow, check that the carrier gas is on, the GC inlet is pressurized, and the column is not plugged. If there is still no flow, consult the Troubleshooting section or contact Technical Support. d. Allow the column to purge for at least 10 minutes. 4 ISQ User Guide Thermo Scientific
15. Perform a column leak check. 1 Changing the Column Replacing the Factory-Installed Column a. Carefully push the capillary column end into the column section of the column-flowmeter connector, as shown in the figure below. CAUTION - INSTRUMENT DAMAGE: Do not allow the column flowmeter connector to exceed 80 °C. Otherwise, it will melt and damage the instrument. Figure 3. Using a Flowmeter for Leak Checks A B C b. Press Leak Check to access the Leak Check Columns menu. Note The available options (Right column or Left column) will differ depending on the configuration of your GC. If you have a FOCUS GC, you do not need to select an option. LEAK CHECK COLUMN Right column Left column < 1. This item appears if the relevant channel is present and configured. c. Scroll to a channel to open the left or right column’s leak check menu. L. COL. LEAK CHECK Start Leak Check Leak Check Settings Thermo Scientific ISQ User Guide 5
Page 1 and 2: ISQ User Guide 1R120555-0003 Revisi
Page 3 and 4: Manufacturer: Thermo Fisher Scienti
Page 5 and 6: WEEE Compliance This product is req
Page 7 and 8: Contents Thermo Scientific ISQ User
Page 9 and 10: Preface About Your System This guid
Page 11 and 12: Related Documentation Preface Relat
Page 13 and 14: Hydrogen Safety Precautions Preface
Page 15 and 16: Hydrogen Connection Guidelines Use
Page 17 and 18: Properly Storing Hydrogen Preface H
Page 19 and 20: Preface Hydrogen Safety Precautions
Page 21 and 22: Changing the Column The ISQ ships w
Page 23: Replacing the Factory-Installed Col
Page 27 and 28: j. Repeat the leak check until no l
Page 29 and 30: i. The following message will indic
Page 31 and 32: 1 Changing the Column Replacing the
Page 33 and 34: Confirming Your GC/MS System is Wor
Page 35 and 36: Checking the Vacuum and Temperature
Page 37 and 38: Tuning the ISQ Tuning will improve
Page 39 and 40: 3 Tuning the ISQ Using ISQ Autotune
Page 41 and 42: 3 Tuning the ISQ Using ISQ Autotune
Page 43 and 44: 9. Click the Print Report button to
Page 45 and 46: 3 Tuning the ISQ Tuning the ISQ wit
Page 51 and 52: Creating a Method Once you’ve got
Page 53 and 54: . Configure the Pre-Injection group
Page 55 and 56: 4 Creating a Method b. Set the MS T
Page 57 and 58: 4 Creating a Method At 4 minutes in
Page 59 and 60: 4 Creating a Method • The Filamen
Page 61 and 62: 4 Creating a Method You can select
Page 63 and 64: 4 Creating a Method Finally, if you
Page 65 and 66: If you have a FOCUS GC: 4 Creating
Page 67 and 68: 4 Creating a Method c. Click the Ca
Page 69 and 70: 4 Creating a Method 6. When you are
Page 71 and 72: Running a Sample Preparing Your Sam
Page 73 and 74: 5 Running a Sample Running a Sequen
Page 75 and 76:
5 Running a Sample Running a Sequen
Page 77 and 78:
Page 79 and 80:
Page 81 and 82:
Page 83 and 84:
e. Configure the Run Synchronously
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Exploring Your Data Once you have a
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6 Exploring Your Data 5. The chroma
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6 Exploring Your Data 8. Right-clic
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6 Exploring Your Data 12. In this s
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6 Exploring Your Data The full scan
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Optimizing Your Method If you did n
Page 97 and 98:
Modifying an Automatic Tune 7 Optim
Page 99 and 100:
6. Configure the General tab. • N
Page 101 and 102:
7 Optimizing Your Method Modifying
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Page 105 and 106:
Page 107 and 108:
Page 109 and 110:
Troubleshooting This section contai
Page 111 and 112:
Investigating Baseline Issues Table
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Table 3. Troubleshooting Peak Issue
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Table 3. Troubleshooting Peak Issue
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Table 4. Troubleshooting Results Is
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8 Troubleshooting Reconfiguring You
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Page 123 and 124:
Page 125 and 126:
16. Click the Done button to close
Page 127 and 128:
Index A acquisition methods, purpos
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tune report, 22, 29 tune types addi
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