ISQ User Guide - Write Frame of Mind

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1 Changing the Column Replacing the Factory-Installed Column d. Scroll to the appropriate column to evaluate and press Enter. The following menu appears if you select the left column and input the physical characteristics of the column. LEFT1 COLUMN INFO Length (m) 15.00 ID (mm) 0.25 Film th. (um) 0.25 1. These settings could also apply to a right column. e. Select Pre/post column? yes (Y) or no (N). • If a pre-/post-column is not present, select N. • If a pre-/post-column is present, select Y. You must set the length and nominal internal diameter of the pre-/post-column in the same valid ranges for the column. The following two lines are added to the menu. Pre/post column? Y P/p col. L 10.00 P/p col. ID 0.530 f. According to the physical characteristics of the column, the system calculates and displays the relevant Column K-factor. LEFT1 COLUMN INFO Column K= (0.8087) Calc’d ID (0.0000) Run Column eval? 1. These settings could also apply to a right column. Note For the most reproducible results, you should conduct a more detailed column evaluation. However, the following steps, while recommended, are not required. g. To evaluate the column, scroll to Run column eval.? and press ENTER. h. Scroll to Measured Flow and input the value of the flow as measured by the flowmeter. EVALUATING L COLUMN Pressure (162) Measured Flow: 4.90 Use to abort Note To abort column evaluation, you can press STOP. 8 ISQ User Guide Thermo Scientific
i. The following message will indicate that evaluation was successful. 1 Changing the Column Replacing the Factory-Installed Column j. Expect a K-factor of approximately 0.7 – 0.9 for a 15 m, 0.25 mm i.d. column (1.3 – 2.0 for a 30 m, 0.25 mm i.d. column). If the column does not report a K-factor within this range or within 0.1 units of the previous stored value, check for a leak or broken column using the leak detector. The K-factor is a measured resistance for the column. A K-factor that is too low may indicate a leak in the system, while a K-factor that is too high may indicate a blockage. 17. Disconnect the column from the column flowmeter connector. 18. Remove the clear plastic component, including its fittings, from the oven and set it aside. 19. Close the GC door. L. COL. EVALUATION COMPLETED Calc’d ID 0.242 K. 0.9020 20. Condition the column before inserting it into the ISQ. CAUTION - INSTRUMENT DAMAGE: The material released from the column (column bleed) during conditioning may contaminate the ion source if the column is inserted into the transfer line before the column is initially conditioned. WARNING - FIRE HAZARD: If you are using a flammable carrier gas, such as hydrogen, it is best to condition the column in the ISQ, even though you may need to clean the ion source afterward. If you can ensure the proper venting of the carrier gas out of the GC to prevent flammable gas buildup, you may proceed at your own risk. a. Run the slow temperature program that is recommended by the manufacturer. A typical program would hold the column at 40 °C for 15 minutes, then ramp at 10 °C/minute up to the maximum allowed column temperature. Hold the column at this temperature for two hours. CAUTION - INSTRUMENT DAMAGE: Never exceed the column manufacturer’s maximum operating temperature. 21. Connect the column to the transfer line. a. Lower the oven temperature to 30 °C and allow it to cool. b. Shut down and vent the ISQ, if necessary. WARNING - BURN HAZARD: The injector, oven, and transfer line may be hot. Allow them to cool to room temperature before touching them. Thermo Scientific ISQ User Guide 9
Page 1 and 2: ISQ User Guide 1R120555-0003 Revisi
Page 3 and 4: Manufacturer: Thermo Fisher Scienti
Page 5 and 6: WEEE Compliance This product is req
Page 7 and 8: Contents Thermo Scientific ISQ User
Page 9 and 10: Preface About Your System This guid
Page 11 and 12: Related Documentation Preface Relat
Page 13 and 14: Hydrogen Safety Precautions Preface
Page 15 and 16: Hydrogen Connection Guidelines Use
Page 17 and 18: Properly Storing Hydrogen Preface H
Page 19 and 20: Preface Hydrogen Safety Precautions
Page 21 and 22: Changing the Column The ISQ ships w
Page 23 and 24: Replacing the Factory-Installed Col
Page 25 and 26: 15. Perform a column leak check. 1
Page 27: j. Repeat the leak check until no l
Page 31 and 32: 1 Changing the Column Replacing the
Page 33 and 34: Confirming Your GC/MS System is Wor
Page 35 and 36: Checking the Vacuum and Temperature
Page 37 and 38: Tuning the ISQ Tuning will improve
Page 39 and 40: 3 Tuning the ISQ Using ISQ Autotune
Page 41 and 42: 3 Tuning the ISQ Using ISQ Autotune
Page 43 and 44: 9. Click the Print Report button to
Page 45 and 46: 3 Tuning the ISQ Tuning the ISQ wit
Page 51 and 52: Creating a Method Once you’ve got
Page 53 and 54: . Configure the Pre-Injection group
Page 55 and 56: 4 Creating a Method b. Set the MS T
Page 57 and 58: 4 Creating a Method At 4 minutes in
Page 59 and 60: 4 Creating a Method • The Filamen
Page 61 and 62: 4 Creating a Method You can select
Page 63 and 64: 4 Creating a Method Finally, if you
Page 65 and 66: If you have a FOCUS GC: 4 Creating
Page 67 and 68: 4 Creating a Method c. Click the Ca
Page 69 and 70: 4 Creating a Method 6. When you are
Page 71 and 72: Running a Sample Preparing Your Sam
Page 73 and 74: 5 Running a Sample Running a Sequen
Page 79 and 80:
5 Running a Sample Running a Sequen
Page 81 and 82:
5 Running a Sample Running a Sequen
Page 83 and 84:
e. Configure the Run Synchronously
Page 85 and 86:
Exploring Your Data Once you have a
Page 87 and 88:
6 Exploring Your Data 5. The chroma
Page 89 and 90:
6 Exploring Your Data 8. Right-clic
Page 91 and 92:
6 Exploring Your Data 12. In this s
Page 93 and 94:
6 Exploring Your Data The full scan
Page 95 and 96:
Optimizing Your Method If you did n
Page 97 and 98:
Modifying an Automatic Tune 7 Optim
Page 99 and 100:
6. Configure the General tab. • N
Page 101 and 102:
7 Optimizing Your Method Modifying
Page 103 and 104:
Page 105 and 106:
Page 107 and 108:
Page 109 and 110:
Troubleshooting This section contai
Page 111 and 112:
Investigating Baseline Issues Table
Page 113 and 114:
Table 3. Troubleshooting Peak Issue
Page 115 and 116:
Table 3. Troubleshooting Peak Issue
Page 117 and 118:
Table 4. Troubleshooting Results Is
Page 119 and 120:
8 Troubleshooting Reconfiguring You
Page 121 and 122:
Page 123 and 124:
Page 125 and 126:
16. Click the Done button to close
Page 127 and 128:
Index A acquisition methods, purpos
Page 129 and 130:
tune report, 22, 29 tune types addi
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