ISQ User Guide - Write Frame of Mind

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1 Changing the Column • Film Thickness—With larger film thicknesses, there is more capacity for the analyte. This capacity can aid in the separation of high concentration samples and in the separation of very volatile samples because thicker stationary phases allow more opportunities for the analytes to interact with the stationary phase. The optimal film thickness depends on the internal diameter of the column. Thick films with small internal diameters will give very strong interactions with the analytes, which can result in longer analysis time and peak tailing. Large ID columns with thin films will have very little interaction with the analytes, which will result in very fast analysis times with little separation. Typical film thicknesses are 0.25 μm for a column with an ID of 0.25 mm. Other common film thicknesses are 0.1, 0.5, and 1.0 μm. • Length—The length of the column affects how much time the analyte has to interact with the stationary phase. Longer columns typically have better resolutions and higher capacities, but longer analysis times. Longer columns are also more expensive. Typical column lengths are 15 or 30 meters for GC/MS, but 100 m columns may be needed for very complex mixtures like gasoline. Very short columns (2.5, 5, and 10 m) are also available. Note Contact your local sales representative to order a Thermo Fisher Scientific column. You can also refer to our catalog or visit our website at www.thermo.com\columns. 2 ISQ User Guide Thermo Scientific
Replacing the Factory-Installed Column To replace the factory-installed column in your ISQ: 1 Changing the Column Replacing the Factory-Installed Column Note If you are running samples, stop the acquisition before powering off the system. 1. Cool down the GC oven and injector. See the GC documentation for information. 2. Open the Xcalibur software and click the Shutdown button in the lower left corner of the Status tab. 3. Click the Yes button to continue the shutdown process. The heaters will be set to 120 °C and when they reach that temperature, the turbomolecular pump is powered off. Then the foreline pump is powered off. 4. Reach around the right side of the instrument and push down on the power switch to power-off the ISQ. Note If you are using hydrogen as a carrier gas, do NOT reach over the top of the instrument to power it off. Instead, reach around the right side or go to the back of the instrument and flip down the power switch. Figure 2. Powering Off the ISQ Vacuum Interlock Shield 5. Open the front door of the instrument. Power Switch Vent Valve Knob 6. Look behind the right side of the vacuum interlock shield and twist the vent valve knob one and a half times in a clockwise direction to open the vent. Thermo Scientific ISQ User Guide 3
Page 1 and 2: ISQ User Guide 1R120555-0003 Revisi
Page 3 and 4: Manufacturer: Thermo Fisher Scienti
Page 5 and 6: WEEE Compliance This product is req
Page 7 and 8: Contents Thermo Scientific ISQ User
Page 9 and 10: Preface About Your System This guid
Page 11 and 12: Related Documentation Preface Relat
Page 13 and 14: Hydrogen Safety Precautions Preface
Page 15 and 16: Hydrogen Connection Guidelines Use
Page 17 and 18: Properly Storing Hydrogen Preface H
Page 19 and 20: Preface Hydrogen Safety Precautions
Page 21: Changing the Column The ISQ ships w
Page 25 and 26: 15. Perform a column leak check. 1
Page 27 and 28: j. Repeat the leak check until no l
Page 29 and 30: i. The following message will indic
Page 31 and 32: 1 Changing the Column Replacing the
Page 33 and 34: Confirming Your GC/MS System is Wor
Page 35 and 36: Checking the Vacuum and Temperature
Page 37 and 38: Tuning the ISQ Tuning will improve
Page 39 and 40: 3 Tuning the ISQ Using ISQ Autotune
Page 41 and 42: 3 Tuning the ISQ Using ISQ Autotune
Page 43 and 44: 9. Click the Print Report button to
Page 45 and 46: 3 Tuning the ISQ Tuning the ISQ wit
Page 51 and 52: Creating a Method Once you’ve got
Page 53 and 54: . Configure the Pre-Injection group
Page 55 and 56: 4 Creating a Method b. Set the MS T
Page 57 and 58: 4 Creating a Method At 4 minutes in
Page 59 and 60: 4 Creating a Method • The Filamen
Page 61 and 62: 4 Creating a Method You can select
Page 63 and 64: 4 Creating a Method Finally, if you
Page 65 and 66: If you have a FOCUS GC: 4 Creating
Page 67 and 68: 4 Creating a Method c. Click the Ca
Page 69 and 70: 4 Creating a Method 6. When you are
Page 71 and 72: Running a Sample Preparing Your Sam
Page 73 and 74:
5 Running a Sample Running a Sequen
Page 75 and 76:
Page 77 and 78:
Page 79 and 80:
Page 81 and 82:
Page 83 and 84:
e. Configure the Run Synchronously
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Exploring Your Data Once you have a
Page 87 and 88:
6 Exploring Your Data 5. The chroma
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6 Exploring Your Data 8. Right-clic
Page 91 and 92:
6 Exploring Your Data 12. In this s
Page 93 and 94:
6 Exploring Your Data The full scan
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Optimizing Your Method If you did n
Page 97 and 98:
Modifying an Automatic Tune 7 Optim
Page 99 and 100:
6. Configure the General tab. • N
Page 101 and 102:
7 Optimizing Your Method Modifying
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Page 107 and 108:
Page 109 and 110:
Troubleshooting This section contai
Page 111 and 112:
Investigating Baseline Issues Table
Page 113 and 114:
Table 3. Troubleshooting Peak Issue
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Table 3. Troubleshooting Peak Issue
Page 117 and 118:
Table 4. Troubleshooting Results Is
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8 Troubleshooting Reconfiguring You
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Page 123 and 124:
Page 125 and 126:
16. Click the Done button to close
Page 127 and 128:
Index A acquisition methods, purpos
Page 129 and 130:
tune report, 22, 29 tune types addi
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