ISQ User Guide - Write Frame of Mind

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8 Troubleshooting Investigating Peak Issues Table 3. Troubleshooting Peak Issues in Your Data, continued Behavior Cause Characteristic Remedy Tailing Sample Peaks Solvent Peaks Chromatographic Column degradation is causing activity Column/oven temperature is too low Inject a test mixture and evaluate the column. Liner is dirty Clean or replace the liner. Glass wool or inlet liner is causing activity Increase the column/oven temperature. Do not exceed the recommended maximum temperature for the stationary phase. Replace wool with fresh silanized wool and install a clean inlet liner. Inlet temperature is too low Increase the temperature of the inlet. Column connections are poor or obstructed Reconnect the column inlet. Stationary phase is wrong Replace the column and choose a more appropriate phase for your analysis. Position of column in inlet is incorrect Reinstall the column. Initial oven temperature is too high (on-column) Reduce the initial oven temperature. Septum purge flow and/or split/splitless vent flow is too low Check and adjust the septum purge and vent flows. Size of injection is too large Reduce the size of your injection. Poor column characterization See Changing the Column for information about checking for leaks and column flow. Bad column Trim, replace, or recondition the column. 94 ISQ User Guide Thermo Scientific
Table 3. Troubleshooting Peak Issues in Your Data, continued Behavior Cause Characteristic Remedy Unresolved Peaks General GC oven is too cold Column has deteriorated Column is not far enough into the transfer line 8 Troubleshooting Investigating Peak Issues The GC oven is not ramped to high enough temperature. Extend upper oven temperature. The GC column needs to be replaced or does not have the appropriate stationary phase for your application. Change GC column. The GC column does not extend into the ISQ source. Use the column measuring tool to confirm the column length. If the end of the column is inside the transfer line, an excessive amount of GC effluent will contact the inside wall. Ion source and/or transfer line are not hot enough. Increase the transfer line temperature. It should be at Transfer line is too cold least as hot as the highest GC oven temperature. Then try increasing the ion source temperature. Sample analyte is absorbing in the GC injector. Clean Injector is too active and deactivate the injection liner. You can also try liners made of different materials. Injection technique is not Choose a more appropriate injection technique. adequate Incorrect column flow rate Make sure the vacuum compensation is turned on. Thermo Scientific ISQ User Guide 95
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ISQ User Guide 1R120555-0003 Revisi
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Manufacturer: Thermo Fisher Scienti
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WEEE Compliance This product is req
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Contents Thermo Scientific ISQ User
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Preface About Your System This guid
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Related Documentation Preface Relat
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Hydrogen Safety Precautions Preface
Page 15 and 16:
Hydrogen Connection Guidelines Use
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Properly Storing Hydrogen Preface H
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Preface Hydrogen Safety Precautions
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Changing the Column The ISQ ships w
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Replacing the Factory-Installed Col
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15. Perform a column leak check. 1
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j. Repeat the leak check until no l
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i. The following message will indic
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1 Changing the Column Replacing the
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Confirming Your GC/MS System is Wor
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Checking the Vacuum and Temperature
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Tuning the ISQ Tuning will improve
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3 Tuning the ISQ Using ISQ Autotune
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3 Tuning the ISQ Using ISQ Autotune
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9. Click the Print Report button to
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3 Tuning the ISQ Tuning the ISQ wit
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Creating a Method Once you’ve got
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. Configure the Pre-Injection group
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4 Creating a Method b. Set the MS T
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4 Creating a Method At 4 minutes in
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4 Creating a Method • The Filamen
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4 Creating a Method You can select
Page 63 and 64: 4 Creating a Method Finally, if you
Page 65 and 66: If you have a FOCUS GC: 4 Creating
Page 67 and 68: 4 Creating a Method c. Click the Ca
Page 69 and 70: 4 Creating a Method 6. When you are
Page 71 and 72: Running a Sample Preparing Your Sam
Page 73 and 74: 5 Running a Sample Running a Sequen
Page 83 and 84: e. Configure the Run Synchronously
Page 85 and 86: Exploring Your Data Once you have a
Page 87 and 88: 6 Exploring Your Data 5. The chroma
Page 89 and 90: 6 Exploring Your Data 8. Right-clic
Page 91 and 92: 6 Exploring Your Data 12. In this s
Page 93 and 94: 6 Exploring Your Data The full scan
Page 95 and 96: Optimizing Your Method If you did n
Page 97 and 98: Modifying an Automatic Tune 7 Optim
Page 99 and 100: 6. Configure the General tab. • N
Page 101 and 102: 7 Optimizing Your Method Modifying
Page 109 and 110: Troubleshooting This section contai
Page 111 and 112: Investigating Baseline Issues Table
Page 113: Table 3. Troubleshooting Peak Issue
Page 117 and 118: Table 4. Troubleshooting Results Is
Page 119 and 120: 8 Troubleshooting Reconfiguring You
Page 125 and 126: 16. Click the Done button to close
Page 127 and 128: Index A acquisition methods, purpos
Page 129 and 130: tune report, 22, 29 tune types addi
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