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Science of Water : Concepts and Applications

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Environmental Biomonitoring, Sampling, <strong>and</strong> Testing 223<br />

TABLE 8.2<br />

Sample Index <strong>of</strong> Macroinvertebrates<br />

Group One<br />

(Sensitive to Pollution)<br />

Group Two<br />

(Somewhat Sensitive to Pollution)<br />

Group Three<br />

(Tolerant <strong>of</strong> Pollution)<br />

Stonefl y larva Alderfl y larva Aquatic worm<br />

Caddisfl y larva Damselfl y larva Midgefl y larva<br />

<strong>Water</strong> penny larva Cranefl y larva Blackfl y larva<br />

Riffl e beetle (adult) Beetle (adult) Leech<br />

Mayfl y larva Dragonfl y larva Snails<br />

Gilled snail Sowbugs<br />

will sampling be accomplished at hourly, daily, weekly, monthly, or even longer intervals? Whatever<br />

sampling frequency <strong>of</strong> sampling is chosen, the entire process will probably continue over a<br />

protracted period (i.e., preparing for biological sampling in the fi eld might take several months from<br />

the initial planning stages to the time when actual sampling occurs). An experienced freshwater<br />

ecologist should be centrally involved in all aspects <strong>of</strong> planning.<br />

The USEPA (2000a) points out that the following issues should be considered in planning the<br />

sampling program:<br />

1. Availability <strong>of</strong> reference conditions for the chosen area<br />

2. Appropriate dates to sample in each season<br />

3. Appropriate sampling gear<br />

4. Availability <strong>of</strong> laboratory facilities<br />

5. Sample storage<br />

6. Data management<br />

7. Appropriate taxonomic keys, metrics, or measurement for macroinvertebrate analysis<br />

8. Habitat assessment consistency<br />

9. A USGS topographical map<br />

10. Familiarity with safety procedures<br />

Once the initial objectives (issues) have been determined <strong>and</strong> the plan devised, then the sampler can<br />

move to other important aspects <strong>of</strong> the sampling procedure. Along with the items just mentioned, it<br />

is imperative that the sampler underst<strong>and</strong> what biological sampling is all about.<br />

Biological sampling allows for rapid <strong>and</strong> general water-quality classifi cation. Rapid classifi cation<br />

is possible because quick <strong>and</strong> easy crosschecking between stream biota <strong>and</strong> a st<strong>and</strong>ard stream<br />

biotic index is possible. Biological sampling is typically used for general water-quality classifi cation<br />

in the fi eld because sophisticated laboratory apparatus is usually not available. Additionally, stream<br />

communities <strong>of</strong>ten show a great deal <strong>of</strong> variation in basic water-quality parameters such as DO,<br />

BOD, suspended solids, <strong>and</strong> coliform bacteria. This occurrence can be observed in eutrophic lakes<br />

that may vary from oxygen saturation to less than 0.5 mg/L in a single day, <strong>and</strong> the concentration<br />

<strong>of</strong> suspended solids may double immediately after a heavy rain. Moreover, the sampling method<br />

chosen must take into account the differences in the habits <strong>and</strong> habitats <strong>of</strong> the aquatic organisms.<br />

Tchobanoglous <strong>and</strong> Schroeder (1985) explain, “Sampling is one <strong>of</strong> the most basic <strong>and</strong> important<br />

aspects <strong>of</strong> water-quality management.”<br />

The fi rst step toward accurate measurement <strong>of</strong> a stream’s water quality is to make sure that<br />

the sampling targets those organisms (i.e., macroinvertebrates) that are most likely to provide the<br />

information that is being sought. Second, it is essential that representative samples be collected.<br />

Laboratory analysis is meaningless if the sample collected is not representative <strong>of</strong> the aquatic

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