Here - EWMA 2013

POSTER PRESENTATIONS
P 218
Poster: Basic Science
TOPICAL APPLICATION OF SUBSTANCE P CAUSED FASTER WOUND HEALING IN
STREPTOZOTOCIN-INDUCED DIABETIC RATS
Vinay Kant 1 , Anu Gopal 1 , Dhirendra Kumar 1 , Anu Gopalakrishnan 1 , Mahendra Ram 1 ,
Raju Prasad 1 , Sadhan Bag 1 , NP Kurade 1 , SK Tandan 1 , Dinesh Kumar 1
1 Indian Veterinary Research Institute (Bareilly, India).
Aim: Diabetes mellitus affects the wound healing process in addition to many other
serious complications. The modalities of treatment to speed up wound healing in diabetics
can result in lesser risk of leg amputation and improve the overall quality of life. The
present study was conducted to evaluate the effects of substance P (SP) in cutaneous
wound in diabetic rats.
Method: Open excision skin wound was created on the back of streptozotocin-induced
diabetic rats and SP (10-6M) was applied topically once daily for 19 days in treatment
group. Photographs, wound area and granulation tissue for expression of VBEGF,
TGF-β1 and histological elucidation were collected on days 3, 7, 14 and 19 postwounding.
Results: SP caused significant increase in wound contraction and decreas wound area,
as compared to untreated diabetic rats. The expressions of VEGF and TGF-β1 in healing
tissue were markedly increased on day 3 and 7 in SP-treated wounds. On histological
examination, increased infiltration of inflammatory cells on day 3, better granulation tissue
with proliferating fibroblasts on day 7 and re-epithelialization on day 14 were well evident
in SP-treated wounds. On day 19, SP-treated wounds also exhibited remodeling and
complete epithelialization, whereas untreated wounds showed presence of macrophages
and absence of epithelial layer.
Conclusions: SP treatment caused faster as well as organized healing of cutaneous
wounds in diabetic rats. There were increased infiltration of inflammatory cells and up
regulation of VEGF and TGF-β1 expression in inflammatory and proliferative phases in
SP-treated diabetic rats.
POSTER: BASIC SCIENCE
P 219
Poster: Basic Science
TOP ICAL P LURONIC F-127 GEL AP P LICATION ENHANCES CUTANEOUS
WOUND HEALING IN RATS
Vinay Kant 1 , Anu Gopal 1 , Dhirendra Kumar 1 , NN Pathak 1 , NP Kurade 1 , SK Tandan 1 ,
Dinesh Kumar 1
1 Indian Veterinary research Institute (IVRI) (Bareilly, India).
Aim: Pluronic F-127 gel is used as vehicle for various topical applications. Here, the
effects of topical application of pluronic F-127 gel on macroscopic and histopthological
changes were evaluated in healing of cutaneous wounds in Wistar rats.
Method: A full-thickness open excision-type skin wound (400 mm 2 ) was created on the
back of rats under anesthesia with pentobarbitone sodium (40 mg/kg). The normal saline
solution and pluronic F-127 gel (25%) were applied topically on wounds in groups 1 and
2, respectively, for 14 days. Photographs, area of wound contraction and granulation
tissue were collected on days 3, 7, 11 and 14 post-wounding.
Results: Photographic evaluation and area of wound contraction revealed that once
daily topical application of gel significantly increased the wound closure after day 7.
Histopathologically, the hematoxylin and eosin stained wound sections showed that early
leukocytes infiltration was more in gel-treated wounds. The early fibroblast proliferation,
angiogenesis and well oriented collagen were markedly better in gel-treated group, as
compared to saline-treated group.
Conclusion: Topical application of pluronic F-127 gel has wound healing potential and
could well be used for the topical delivery of the various wound healing drugs to enhance
healing.
Key words: Pluronic F-127 gel, wound healing, rats.
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