Here - EWMA 2013

POSTER PRESENTATIONS
P 309
HAIR EXTRACTIVE TYPOLOGY AND MICROBIAL CONTAMINATION IN THE
SURGICAL PREPARATION OF SKIN BEDDING
Poster: Infection
Bienvenida Rodríguez-de-Vera 1 , Carmelo Monzón-Moreno 1 ,
Juan Fernando Jiménez-Díaz 1 , Rodrigo Chacón-Ferrera 1 ,
Franciso José Hernandez-Martínez 1 , Carla Jiménez-Rodríguez 1
1 University of Las Palmas de Gran Canaria (Las Palmas de Gran Canaria, Spain).
Aim: Traditionally it has been considered that mechanical extraction methods were
more aggressive for skin than the use of chemical ones, like chemical depilatories.
Method: They conducted an experimental study of the impact of the extraction
methodology of hair at dorsal level in experimental animals (white rats, Sprague Dawley
strain), in order to determine microbial contamination and the microbial type that
develops in the skin. Animals were divided into four groups. Different hair removal
techniques were applied to each group (using scissors, by manual shaving, electric
shaving or waxing).Samples of skin using hysopo of each group of animals were taken,
then seeded and subjected to a bacteriological culture in order to determine the number
and type of colonies that developed, following the extractive hair methodology used.
Results: The use of an electric shaver was the less contaminant extractive hair method
with an average of 4,000 cfu, followed by the use of manual razor with an average of
half of 8000 cfu. The use of scissors shows higher figures (average of 22,000 cfu) as
well as the depilatory method (average of 53,000 cfu). The four hair extraction methods
used in our experiment were mainly contaminated by strains of coagulase-negative
staphylococci.
Conclusions: Hair extractive methodology does not change the type of microbial
colonization of the skin but its quantification. As a result, chemical methods are more
contaminant than mechanical ones
POSTER: INFECTION
P 310
PIPERACILLIN/TAZOBACTAM-INDUCED NEUTROPENIA IN PATIENTS WITH
DIABETIC FOOT INFECTION
Poster: Infection
Gunalp Uzun 1 , Mesut Mutluoglu 1 , Asım Ülçay 2 , Ahmet Karakaş 3 , Hakan Ay 1 ,
Vedat Turhan 2
1 GATA Haydarpasa Teaching Hospital Department of Underwater and Hyperbaric
Medicine (Istanbul, Turkey);
2 GATA Haydarpasa Teaching Hospital Department of Infectious Disease and Clinical
Microbiology (Istanbul, Turkey);
3 GATA Department of Infectious Disease and Clinical Microbiology (Ankara, Turkey).
Aim: Piperacillin/tazobactam (PTZ) is frequently used in patients with diabetic foot
infections. PTZ may induce severe neutropenia if used for prolonged durations.
However, current guidelines recommending the use of PTZ in diabetic foot infections
provide limited information about this adverse effect. The aim of this study was to
investigate the prevalence of PTZ induced neutropenia in patients with diabetic foot
infections.
Methods: We retrospectively reviewed the hospital records of patients who received
PTZ for diabetic foot infections between January 1st, 2006 and December 31st, 2012.
Patients who received PTZ for 10 days or more were included. Neutropenia was defined
as an absolute neutrophil count of 2000 cells/mm 3 . Neutropenia is accepted as PTZ
related, if it developed during PTZ treatment and disappeared with the cessation of PTZ,
and if no other reason for neutropenia is found.
Results: During the study period, 29 patients with diabetic foot infection have received
PTZ for 10 days or more. There were 13 females and 16 males. The mean age of the
patients was 65.9 +/- 9.7 years. One patient was hospitalized for 2 times. PTZ related
neutropenia was found in 3 of 30 treatments (10%). Fever accompanied neutropenia in
all three patients. Neutropenia disappeared in 5-7 days after the cessation of PTZ.
Conclusion: Neutropenia developed in 1 out of 10 patients who received PTZ for 10
days or more. Clinicians should be aware of this side effect if long-term PTZ use is
planned in patients with diabetic foot infections.
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