Here - EWMA 2013

POSTER PRESENTATIONS
P 290
TREATMENT OF MYCOSIS IN SKIN FOLDS
Poster: Dressings
Astrid Probst 1 , Amrei Steinhoff 2 , Julia Otte 2
1 Klinikum am Steinenberg (Reutlingen, Germany);
2 BSN medical (Hamburg, Germany).
Introduction and Aim: Mycoses are infections that affect the skin and mucosa and are
provoked by particular fungi that proliferate in a warm damp environment. They appear
with symptoms such as redness of the skin, itching, burning, dryness and flaking or
maceration and exudation. Mycoses often affect the folds of the skin, where there is
poorer ventilation as sub-mammary, inguinal or abdominal skin folds. The effectiveness
of a hydrophobic wound dressing that does not contain any active antimicrobial agents
should be demonstrated for different parts of the body.
Methods: For the treatment of fungal infections a hydrophobic dressing* was used on
different kind of skin folds for several days. Infected areas were covered with the
hydrophobic wound dressing* and changed on a regular daily basis. No additional anti
fungal medication was used.
Results: The presented case reports show a reduction in signs of infection and a
healing of the affected skin folds. After a few days period, redness was gone or almost
gone in all cases shown. Fungal infection in sub-mammary, inguinal or abdominal skin
folds could be treated successfully without using any antimicrobial agents, creams or
ointments.
Conclusion: Mycosis in skin folds is often distracting for patients as they appear with
various symptoms. The hydrophobic wound dressing* binds and removes the fungi from
the wounds effectively leading to reduced symptoms and curing of the skin. This makes
the dressing a safe and convenient treatment for fungal infections in skin folds.
*Cutimed ® Sorbact ® , BSN medical
POSTER: DRESSINGS
P 291
Poster: Dressings
NEW WOUND DRESSING COMBINING A GALACTOMANNAN-BASED ABSORBENT
MATRIX AND AN ANTIOXIDANT HYDRATION SOLUTION FOR PROTECTING
TISSUES FROM AN OXIDATIVE ENVIRONMENT
Javier Soldevilla 3 , Begoña Castro Feo 1 , A Azcoitia 1 , A Alonso-Varona 2 , A Baiget 1 ,
J Herrero 1 , M del Olmo 1 , F Bastida 4 , T Palomares 2
1
Histocell. Bizkaia Technologic Park. Basque Country (Spain);
2
Faculty of Medicine. University of the Basque Country, Basque Country (Spain));
3
GNEAUPP, National Advisory Group for the Study of Pressure Ulcers and Chronic
Wounds (Spain);
4
ArtinVet. Bizkaia Technologic Park. Basque Country (Spain).
Aim: To evaluate the effect of a new wound dressing concept to protect cells from one of
the relevant events that contributes to the chronification process in a wound, a recurrent
oxidative environment. The dressing includes a galactomannan-based absorbent matrix
and an antioxidant hydration solution. In this work, results from matrix properties and
solution efficacy in damaged human primary fibroblast are presented.
Methods: A human fibroblast oxidative stress injury model based on the addition of
H2O2 was established and characterized in relation to cell viability, cell proliferation,
COX-2, NFβ, and ROS expression levels. This model was used to determine the
effects of the components in the hydration solution (N-acetyl cysteine – NAC- and
curcumin) on injured cells. Physico-structural and cytotoxicity of the matrix were
analyzed using SEM, EWC, and MTT based cytotoxicity.
Results: In our model, intracellular ROS production of oxidative stressed cells (H2O2)
was reduced over 90% in the presence of the hydration solution (NAC and curcumin).
These components also rescued cell viability up to non-oxidated control levels, showing
a strong cell protective effect. SEM analyses of the galactomannan structure showed a
homogeneous interconnected porosity with pores of around 200 µm in diameter, which
facilitates the exchange of nutrients and gases. Also, the matrix was capable of
absorbing more than 20 times its dry weight and was not cytotoxic.
Conclusions: The new dressing containing NAC and curcumin shows inexistent
cytotoxicity and antioxidant capabilities that protect the cells from an oxidative
environment.
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