Here - EWMA 2013

POSTER PRESENTATIONS
P 270
Poster: Dressings
IN VITRO EVALUATION OF MMP BINDING CAPACITY OF A SUPERABSORBENT
DRESSING* AND THE REDUCTION OF COLLAGENASE ACTIVITY
Cornelia Wiegand 1 , Uta-Christina Hipler 1
1 Department of Dermatology, University Medical Center Jena (Jena, Germany).
Aim: Chronic wounds contain elevated levels of matrix metalloproteinases (MMPs),
degrading matrix and preventing wound closure. Wound management focuses on
reducing inflammatory mediators and establishing a moist environment. Superabsorbent
dressings can take up a multiple amount of fluid while providing moisture to the wound.
The aim of this study was to investigate the binding capacity of a superabsorbent
dressing* for MMP-2 and MMP-9 and inhibition of collagenase activity in vitro.
Methods: Dressing samples* were cut into pieces, taken in a final volume of 1mL of
protease solution (MMP-2:5000pg/mL, MMP-9:2000pg/mL, collagenase:0.2U/mL), and
incubated up to 24h at 37°C. Supernatants were collected and bound protein was eluted
from dressing samples. Concentrations of MMPs in supernatants and eluates were
determined by specific immunoassays.
Results: It was shown that the superabsorbent dressing* possesses a significant
binding capacity for MMP-2 and MMP-9 (p