Here - EWMA 2013

P 281
Poster: Dressings
A NOVEL QUANTITATIVE CZOI BIOFILM ASSAY BASED ON 3D SOFT TISSUE
Linnea Karlsson Lönnberg 1 , Eva Larkö 1 , Kristina Blom 1 , Bodil Hakonen 1
1 Medibiome AB (Mölnlycke, Sweden).
Aim: To develop a method mimicking reality based on 3D soft tissue where biofilm is
formed and the antimicrobial activity of wound dressings can be screened and compared
qualitatively and quantitatively.
Method: 3D soft tissue (allowing biofilm formation) was made and inoculated with
Pseudomonas aeruginosa before application of dressings. After incubation for 24h,
corrected zone of inhibition (CZOI) was viewed and measured. Thereafter, the bacterial
burden in dressings and soft tissues were analyzed separately. CZOI analyses run on
Mueller Hinton agar (MHA) were run for comparison. Dressings included were: silver
containing hydrofiber (SCH) and silver containing foams (SCFI and SCFII (± prehydration))
and their counterparts without silver.
Results: CZOI could be viewed on 3D soft tissues and found bigger for the SCFs than
for the SCH. The opposite was observed when using MHA where the CZOI was bigger
for the SCH unless the SCFII was pre-hydrated. The bacterial burden in the dressings
and in the soft tissues could be analyzed and was reduced with several logs for the
SCFs and less than 1 log for the SCH compared with their counterparts.
Conclusion: This biofilm method based on 3D soft tissue offers an in vivo relevant
model to qualitatively and quantitatively assess the antimicrobial activity of wound
dressing independent of their physical properties and without the need for pre-hydration.
Results could reflect the accessibility of silver to act on biofilm; SCH contains 10x less
silver than the SCFs.
Poster: Dressings
P 280
Who is who in chronic wound dressing. A new pragmatic algorithm.
Bernd Gächter 1 , Sebastian Probst 2 , Stephane Schlunke 1 , Paul Biegger 1
1 Institute for surgery / ospedale regionale di locarno (Locarno, Switzerland);
2 ZHAW Zürcher Hochschule für Angewandte Wissenschaften (Winterthur, Switzerland).
Aim: The aim of this study is to present an algorithm, that was applied by all medical
and non-medical staff that reduced the number of possible „errors“ as well as the
consumption costs by 30% in our wound outpatient clinic as in daily practice of
outpatients and hospital care of chronic wounds, the plethora of possible dressings and
treatments often leads to misunderstanding and confusion.
Methods: A pre- and post-design was used to test a wound algoritm. Based on a
decennial experience in our wound ambulatory (selected and exemplary cases will be
presented) and on current literature, we developed and confirmed a new decisional
algorithm for daily practice for inpatients and outpatients.
Results: The clinical and economical outcome of our patients the year before and after
the application of the above mentioned algorithm, showing a net benefit of 20% faster
ulcer healing and 30% cheaper treatment was measured. A detailed classification of our
patient population and the ready to use algorithm will be presented.
Conclusions: As seen lately with the application of simple measures like a preoperative
check-list based on the World Health Organization (WHO), our treatment
algorithm does warrant us very satisfying results.
POSTER: DRESSINGS
EWMA 2013
COPENHAGEN
15-17 May · 2013
Danish Wound
Healing Society
169