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E-POSTER PRESENTATIONS<br />
EP 488<br />
IN VITRO ASSESSMENT OF ABSORBENCY AND RETENTION OF<br />
MICROORGANISMS<br />
E-Poster: Dressings<br />
Valerie Edwards-Jones 1 , Pam Spruce 2<br />
1 Manchester Metropolitan University (Manchester, United Kingdom);<br />
2 TVRE Consulting (Stoke-On-Trent, United Kingdom).<br />
Chronic wound exudate contains harmful microorganisms which can delay healing.<br />
Absorbent dressings are used to manage exudate and retain microorganisms.<br />
Aim: To determine the absorbency and retention of microorganisms by 4 dressings.<br />
Methods: Phosphate buffered saline containing a known number of microorganisms<br />
was absorbed into 4 different wound dressings under 60mm Hg pressure. The amount<br />
of fluid and numbers of microorganisms absorbed into the dressings was determined at<br />
4 and 24hrs. Four microorganisms were used, Escherichia coli, Pseudomonas<br />
aeruginosa, methicillin resistant Staphylococcus aureus and Candida albicans:<br />
Results: A similar amount of fluid was absorbed by all four dressings at 4hrs, but<br />
dressing 2** absorbed the highest volume over the 24hr period.<br />
The sequestration and retention of microorganisms into the dressings varied depending<br />
upon microorganism and the time period.<br />
At 4hrs, Dressing 3*** retained more E.coli, Dressing 4**** retained more P.aeruginosa<br />
and Candida albicans, and Dressing 2** retained more MRSA.<br />
At 24hrs, Dressing 3*** retained more Gram negative bacteria, than the other dressings<br />
and Dressing 2** retained more MRSA and Candida albicans than the other dressings.<br />
Conclusions: The absorbency of the dressings varied over the two different time<br />
periods. Retention of microorganisms in the dressing varied depending upon their Gram<br />
type. This may have been due to an electrostatic interaction between the microorganism<br />
and the dressing.<br />
* Advadraw (Advencis), ** Drawtex (Beier), *** Aquacel Extra (Convatec), *****Urgoclean (UrgoMedical)<br />
E-POSTER: DRESSINGS<br />
EP 489<br />
E-Poster: Dressings<br />
Guidelines for the use of an incision management system after<br />
median sternotomy<br />
Ammar Mustafa 1 , Shady Ashraf 1 , Nasser Mughal 1 , Cornelia Carr 1 , Abdulaziz Alkhulaifi 1<br />
1 Heart Hospital, Hamad Medical Corporation (Doha, Qatar).<br />
Guidelines for the use of Negative Pressure Wound Therapy (NPWT) after Cardiac<br />
Surgery<br />
Postoperative wound complications following cardiac surgery, deep sternal infections in<br />
particular, are associated with increased morbidity and mortality. Negative Pressure<br />
Wound Therapy (NPWT) is well established in the management of infected sternal<br />
wounds. However, it is a relatively new modality of treatment for clean and surgical<br />
incisions. We have been utilizing NPWT for the last 6 months in the treatment of<br />
sternotomy incisions in patients considered high risk for complications such as surgical<br />
site infection and wound dehiscence.<br />
There are no specific clinical indications in the literature for the use of NPWT following<br />
cardiac surgery. We have looked at our 6 months experience with this type of dressing to<br />
check if we can define some indications.<br />
We propose the following clinical guidelines for the use of NPWT post cardiac surgery: 1.<br />
Obese patients with Body Mass Index >30. 2. Uncontrolled diabetes (HbA1c>8%). 3.<br />
Bilateral internal mammary artery harvesting. 4. Heavy smoking and COPD. 5. Fragile<br />
sternum and fractured sternum during sternotomy. 6. Delayed primary closure of sternal<br />
wound. 7. Further meian sternotomies after first time (for “redo” operations).<br />
We applied NPWT to 47 patients with one or more of these criteria immediately after<br />
closure of the sternotomy wound. The dressing was removed after 6-7 days. The primary<br />
end point was the development of wound complications within 30 days. 1 patient<br />
developed a sternal wound infection (2.1%) compared to 5 patients (10.6%) from a<br />
similar control group of 47 patients (no NPWT).<br />
We have also used NPWT after secondary closure of 3 thigh wounds that had to have<br />
haematomas evacuated at the sites of long saphenous vein harvesting for Coronary<br />
Artery Bypass Grafting (CABG) with good results. In conclusion, NPWT may have<br />
additional benefits in preventing complications in high risk patients as a primary or a<br />
secondary dressing. We are currently planning for further studies regarding the use of<br />
NPWT for clean surgical incisions.<br />
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