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E-POSTER PRESENTATIONS<br />

EP 488<br />

IN VITRO ASSESSMENT OF ABSORBENCY AND RETENTION OF<br />

MICROORGANISMS<br />

E-Poster: Dressings<br />

Valerie Edwards-Jones 1 , Pam Spruce 2<br />

1 Manchester Metropolitan University (Manchester, United Kingdom);<br />

2 TVRE Consulting (Stoke-On-Trent, United Kingdom).<br />

Chronic wound exudate contains harmful microorganisms which can delay healing.<br />

Absorbent dressings are used to manage exudate and retain microorganisms.<br />

Aim: To determine the absorbency and retention of microorganisms by 4 dressings.<br />

Methods: Phosphate buffered saline containing a known number of microorganisms<br />

was absorbed into 4 different wound dressings under 60mm Hg pressure. The amount<br />

of fluid and numbers of microorganisms absorbed into the dressings was determined at<br />

4 and 24hrs. Four microorganisms were used, Escherichia coli, Pseudomonas<br />

aeruginosa, methicillin resistant Staphylococcus aureus and Candida albicans:<br />

Results: A similar amount of fluid was absorbed by all four dressings at 4hrs, but<br />

dressing 2** absorbed the highest volume over the 24hr period.<br />

The sequestration and retention of microorganisms into the dressings varied depending<br />

upon microorganism and the time period.<br />

At 4hrs, Dressing 3*** retained more E.coli, Dressing 4**** retained more P.aeruginosa<br />

and Candida albicans, and Dressing 2** retained more MRSA.<br />

At 24hrs, Dressing 3*** retained more Gram negative bacteria, than the other dressings<br />

and Dressing 2** retained more MRSA and Candida albicans than the other dressings.<br />

Conclusions: The absorbency of the dressings varied over the two different time<br />

periods. Retention of microorganisms in the dressing varied depending upon their Gram<br />

type. This may have been due to an electrostatic interaction between the microorganism<br />

and the dressing.<br />

* Advadraw (Advencis), ** Drawtex (Beier), *** Aquacel Extra (Convatec), *****Urgoclean (UrgoMedical)<br />

E-POSTER: DRESSINGS<br />

EP 489<br />

E-Poster: Dressings<br />

Guidelines for the use of an incision management system after<br />

median sternotomy<br />

Ammar Mustafa 1 , Shady Ashraf 1 , Nasser Mughal 1 , Cornelia Carr 1 , Abdulaziz Alkhulaifi 1<br />

1 Heart Hospital, Hamad Medical Corporation (Doha, Qatar).<br />

Guidelines for the use of Negative Pressure Wound Therapy (NPWT) after Cardiac<br />

Surgery<br />

Postoperative wound complications following cardiac surgery, deep sternal infections in<br />

particular, are associated with increased morbidity and mortality. Negative Pressure<br />

Wound Therapy (NPWT) is well established in the management of infected sternal<br />

wounds. However, it is a relatively new modality of treatment for clean and surgical<br />

incisions. We have been utilizing NPWT for the last 6 months in the treatment of<br />

sternotomy incisions in patients considered high risk for complications such as surgical<br />

site infection and wound dehiscence.<br />

There are no specific clinical indications in the literature for the use of NPWT following<br />

cardiac surgery. We have looked at our 6 months experience with this type of dressing to<br />

check if we can define some indications.<br />

We propose the following clinical guidelines for the use of NPWT post cardiac surgery: 1.<br />

Obese patients with Body Mass Index >30. 2. Uncontrolled diabetes (HbA1c>8%). 3.<br />

Bilateral internal mammary artery harvesting. 4. Heavy smoking and COPD. 5. Fragile<br />

sternum and fractured sternum during sternotomy. 6. Delayed primary closure of sternal<br />

wound. 7. Further meian sternotomies after first time (for “redo” operations).<br />

We applied NPWT to 47 patients with one or more of these criteria immediately after<br />

closure of the sternotomy wound. The dressing was removed after 6-7 days. The primary<br />

end point was the development of wound complications within 30 days. 1 patient<br />

developed a sternal wound infection (2.1%) compared to 5 patients (10.6%) from a<br />

similar control group of 47 patients (no NPWT).<br />

We have also used NPWT after secondary closure of 3 thigh wounds that had to have<br />

haematomas evacuated at the sites of long saphenous vein harvesting for Coronary<br />

Artery Bypass Grafting (CABG) with good results. In conclusion, NPWT may have<br />

additional benefits in preventing complications in high risk patients as a primary or a<br />

secondary dressing. We are currently planning for further studies regarding the use of<br />

NPWT for clean surgical incisions.<br />

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