Here - EWMA 2013

POSTER PRESENTATIONS
P 258
In-Vitro assessment of fluid handling properties of
Chlorhexidine Gluconate containing dressings for
the protection of peri-wound skin
Poster: Dressings
David Warde 1 , Andrew Hoggarth 1 , Andy Darby 1 , Rachel Malone 1 , Craig Hardy 1
1 Medtrade Products Ltd (Crewe, United Kingdom).
Introduction: The introduction of an antibacterial disc, containing Chlorhexidine
Gluconate (CHG) which can be placed around the catheter has been shown to reduce
the incidence of Catheter-Related Blood Stream Infections by 60% 1 . This study
demonstrates the in-vitro performance of a newly developed and FDA approved CHG
disc compared to the existing marketed Protective Disc with CHG.
Test Methods: The methods used to assess the CHG containing discs, included fluid
absorbency analysing the discs ability to absorb wound exudate, the elution profile of
the Chlorhexidine Gluconate from the substrate indicating availability to the bacterial
organisms. Fluid absorbency assessed the speed at which fluid was absorbed into the
dressing and the total volume. The elution methods was developed to assess the
amount of CHG that was eluted from the dressing following fluid absorption over a 7 day
period under different saturation limits.
Results: The results 2 for the elution profile of the CHG was greater for the CHG disc
compared to the Protective disc with CHG for all saturation levels. The elution profile of
the CHG was double that of the Protective Disc with CHG over the first two days then
comparable over the remaining days. This indicates more available CHG, irrespective of
the moisture levels, within the dressing. The absorbency was also greater for the CHG
disc, indicating greater fluid handing. The elution profile of the CHG disc at each
timepoint was greater than the MIC of the CHG.
Conclusion: The data from this study shows that the performance profile of the new
CHG disc exceeds that of the existing Protective disc with CHG, in terms of handling
more wound fluid and making available higher concentrations of CHG to aid reduction of
the bacterial load.
References:
1. Hendley JO, Ashe KM. Effect of topical antimicrobial treatment on aerobic bacteria in the stratum corneum of
human skin Antimicrobial Agents and Chemotherapy. April 1991;35(4):627-631
2. Data on file
POSTER: DRESSINGS
P 259
Poster: Dressings
In-Vitro assessment of antibacterial activity of Chlorhexidine
Gluconate containing dressings for the protection of peri-wound
skin
Andrew Hoggarth 1 , Peter Walker 1 , Andy Darby 1 , Rachel Malone 1 , Craig Hardy 1
1 Medtrade Products Ltd (Crewe, United Kingdom).
Introduction: A known problem related to the use of central venous and arterial
catheters is the incidence of Catheter-Related Blood Stream Infections (CRBSI), local
infections and skin colonization of bacterial. This study assesses the antibacterial
efficacy of a newly developed and FDA approved CHG disc compared to an existing
marketed Protective Disc with CHG.
Test Methods: The methods used to assess antimicrobial activity was a combination of
a direct contact kill study and zone of inhibition.
The organisms tested were gram +ve, gram –ve and fungi. For the direct kill method the
samples were applied with the nutrient broth of appropriate organism ensuring the
inoculums were absorbed into the dressing. The samples were incubated for each time
point, removed and rinsed. The dilutions were plated onto plates and incubated for 3-5
days. The plates were counted for number of colonies.
Results: The results show that both the CHG disc and the Protective disc with CHG
demonstrated antimicrobial activity over the 7 day period and had similar bacterial kill
rates. The data supports a log 4 kill rate at day 6 when re-challenged with bacterial.
Conclusion: The data from this study shows that the performance profile of the new
CHG disc is equivalent to that of the existing Protective disc with CHG, indicating that it
may also help reduce the incidence of Catheter-Related Blood Stream Infections.
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