112that a unit <strong>in</strong>crease <strong>in</strong> water temperature resulted <strong>in</strong>to a significant decrease(P
113MPP016Regulation and functional characterization of the Arg<strong>in</strong><strong>in</strong>eDeim<strong>in</strong>ase System (ADS) of pyogenic streptococciA. Hitzmann* 1 , M. Rohde 1 , O. Goldmann 1 , S. Bergmann 2 , G.S. Chhatwal 1 ,M. Fulde 31 Helmholtz Centre for Infection Research, Medical Microbiology,Braunschweig, Germany2 TU Braunschweig, Braunschweig, Germany3 Hannover Medical School, Hannover, GermanyPyogenic streptococci comprise a large family of pathogenic bacteria<strong>in</strong>clud<strong>in</strong>g human specific, animal specific, and zoonotic species, lead<strong>in</strong>g tosimilar cl<strong>in</strong>ical patterns and diseases. Its spectrums of diseases range frommild <strong>in</strong>fections of the sk<strong>in</strong> to severe and life-threaten<strong>in</strong>g septicaemia,necrotiz<strong>in</strong>g fasciitis and toxic shock-like syndrome. Similarities <strong>in</strong> thepatho-physiology between S. canis, ma<strong>in</strong>ly isolated from dogs and cats,but <strong>in</strong>creas<strong>in</strong>gly recognized as a zoonotic agent, and the well characterizedhuman pathogens S. pyogenes and S. dysgalactiae sub. equismilis suggestcommon virulence factors <strong>in</strong> these species. One of these virulence traits isthe Arg<strong>in</strong><strong>in</strong>e Deim<strong>in</strong>ase System (ADS) which is widely distributed amongpathogenic streptococci and other prokaryotes. As a secondary metabolicpathway, the ADS catalyses the conversion from arg<strong>in</strong><strong>in</strong>e to ornith<strong>in</strong>e,thereby produc<strong>in</strong>g ATP, CO 2, and ammonia. Besides its role <strong>in</strong>metabolism, the ADS is also upregulated dur<strong>in</strong>g <strong>in</strong>fection of the host.Moreover, it is speculated that the ability to raise the external pH due toammonia formation dur<strong>in</strong>g arg<strong>in</strong>olysis is responsible for overcom<strong>in</strong>gacidic conditions, e.g. <strong>in</strong> the phagolysosome.In pyogenic streptococci the ADS consists of seven genes: arcR and flpS,cod<strong>in</strong>g for putative transcriptional regulators, arcA, an arg<strong>in</strong><strong>in</strong>e deim<strong>in</strong>ase,arcB, an ornith<strong>in</strong>e-carbamoyltransferase, arcC, a carbamate k<strong>in</strong>ase, arcD,an arg<strong>in</strong><strong>in</strong>e-ornith<strong>in</strong>e antiporter, and arcT, a putative Xaa/His-dipeptidase.We could show <strong>in</strong> RT-PCR and Western Blot analysis, as well as <strong>in</strong>enzymatic assays, that the ADS is highly upregulated under nutrientstarvation and arg<strong>in</strong><strong>in</strong>e supplementation. Furthermore, glucose effectivelyrepresses the ADS expression underl<strong>in</strong><strong>in</strong>g its function as a secondarymetabolic pathway. Prelim<strong>in</strong>ary phenotypic analysis us<strong>in</strong>g FACS andelectron microscopy revealed that ArcA, ArcB, and ArcC are located onthe bacterial surface. This would be a prerequisite for neutraliz<strong>in</strong>genvironmental acidification. However, its exact contributions to virulencerema<strong>in</strong> elusive.MPP017Borrelia bavariensis sp. nov. resist complement-mediatedkill<strong>in</strong>g <strong>in</strong>dependent of b<strong>in</strong>d<strong>in</strong>g of complement regulatorsC. Hammerschmidt* 1 , A. Koenigs 1 , T. Hallström 2 , C. Skerka 2 , R. Wallich 3 ,P.F. Zipfel 2,4 , P. Kraiczy 11 University Hospital Frankfurt, Medical Microbiology and InfectionControl, Frankfurt, Germany2 Leibniz Institute for Natural Product Research and Infection Biology,Department of Infection Biology, Jena, Germany3 University of Heidelberg, Institute of Immunology, Heidelberg, Germany4 Friedrich Schiller University, Jena, GermanyLyme disease, the most prevalent vector-borne anthropozoonosis <strong>in</strong>Europe, is caused by spirochetes of the Borrelia burgdorferi sensu latocomplex.B. burgdorferi sensu lato differ <strong>in</strong> their resistance to complement-mediatedkill<strong>in</strong>g by human serum. It is well-known that complement resistance iscorrelated with the ability of Borreliae to b<strong>in</strong>d host-derived fluid-phasecomplement regulators of the alternative pathway, factor H (CFH) andfactor H-like prote<strong>in</strong> 1 (FHL-1) via dist<strong>in</strong>ct molecules termed complementregulator-acquir<strong>in</strong>g surface prote<strong>in</strong>s or CRASPs.Here, we <strong>in</strong>vestigate Borrelia bavariensis sp. nov. formerly described asBorrelia gar<strong>in</strong>ii OspA serotype 4 for elucidat<strong>in</strong>g the molecular mechanismof serum resistance. This genospecies showed a higher pathogenicity tohumans and displayed an <strong>in</strong>termediate serum-resistant phenotype to humanserum. Interest<strong>in</strong>gly, none of the Borrelia bavariensis stra<strong>in</strong>s analyzedwere able to acquire complement regulators CFH or FHL-1 and did notproduce any CRASPs.To exclude the possibility that B. bavariensis captures complementregulators of the classical pathway to escape the <strong>in</strong>nate immune system,the capacity of several isolates to b<strong>in</strong>d C4b-b<strong>in</strong>d<strong>in</strong>g prote<strong>in</strong> (C4BP) or C1-Inhibitor was exam<strong>in</strong>ed. S<strong>in</strong>ce none of the borrelial isolates were able tob<strong>in</strong>d these complement regulators, we <strong>in</strong>vestigated two CRASP-1orthologous prote<strong>in</strong>s, BGA66 and BGA71 display<strong>in</strong>g an <strong>in</strong>tr<strong>in</strong>siccomplement regulatory activity.Therefore, a serum-sensitive B. gar<strong>in</strong>ii stra<strong>in</strong> lack<strong>in</strong>g all CRASPs wastransformed with a shuttle vector harbor<strong>in</strong>g the entire BGA66 or BGA71encod<strong>in</strong>g gene under the control of their native promoters. Apply<strong>in</strong>ggrowth <strong>in</strong>hibition assays, the borrelial transformants G1/pBGA66 andG1/pBGA71 survived <strong>in</strong> 50% human serum. In addition, both stra<strong>in</strong>s alsoshowed a strongly reduced deposition of complement components on theirsurface when compared to the wild-type stra<strong>in</strong> suggest<strong>in</strong>g that BGA66 andBGA71 exhibit complement regulatory activity.Taken together, we demonstrate that B. bavariensis sp. nov. survives <strong>in</strong>human serum <strong>in</strong>dependently of its ability to b<strong>in</strong>d diverse complementregulators <strong>in</strong>clud<strong>in</strong>g CFH, FHL-1, CFHR-1, CFHR-2, CFHR-5, C4BP, andC1-Inhibitor. In addition BGA66 and BGA71 were identified as candidatesfor facilitat<strong>in</strong>g serum resistance of B. bavariensis.MPP018Phosphosignal<strong>in</strong>g of human bronchial epithelial cells <strong>in</strong>response to bacterial virulence factorsE. Richter* 1 , M. Harms 1 , K. Ventz 1 , J.-P. Jan-Peter Hildebrandt 2 , J. Mostertz 1 ,F. Hochgräfe 11 Greifswald University, Pathoproteomics, Greifswald, Germany2 Greifswald University, Animal Physiology and Biochemistry, ZoologicalInstitute, Greifswald, GermanyBackground: The gram-positive bacterium Staphylococcus aureus is awidespread pathogen that colonizes the human sk<strong>in</strong> and the upperrespiratory tract. It can cause community- and hospital-acquired <strong>in</strong>fections,<strong>in</strong>clud<strong>in</strong>g endocarditis, pneumonia, or even sepsis. On the cellular level, S.aureus is able to <strong>in</strong>vade host cells and evades the immune response.Methods: Here, we have employed stable isotope label<strong>in</strong>g with am<strong>in</strong>oacids <strong>in</strong> cell culture (SILAC), enrichment of phosphorylated prote<strong>in</strong>s andhigh-accuracy quantitative mass spectrometry <strong>in</strong> order to def<strong>in</strong>e the hostcell response of human bronchial epithelial cells to virulence factors anddur<strong>in</strong>g <strong>in</strong>vasion and post-<strong>in</strong>vasion by staphylococci.Conclusion: Human bronchial epithelial cells represent a first l<strong>in</strong>e ofdefense aga<strong>in</strong>st <strong>in</strong>vad<strong>in</strong>g pathogens. Sens<strong>in</strong>g of bacterial products or direct<strong>in</strong>teraction with the pathogenic aggressor leads to a def<strong>in</strong>ed change ofsignal perception and transduction and eventually results <strong>in</strong> areprogrammed cellular activity.MPP019The evolution of Zygomycetes as causative agents of emergentdiseasesK. Voigt* 1,2 , K. Hoffmann 1,2 , V.U. Schwartze 1,2 , I.D. Jacobsen 1 , G.S. de Hoog 31 Leibniz Institute for Natural Product Research and Infection Biology, Jena,Germany2 University of Jena, Dept. Microbiology and Molecular Biology, Jena, Germany3 CBS-KNAW Fungal Biodiversity Centre, Utrecht, NetherlandsZygomycetes, formerly described as class with<strong>in</strong> the fungal k<strong>in</strong>gdom, arepolyphyletic, and therefore, split <strong>in</strong>to five dist<strong>in</strong>ct subphyla, which are theEntomophthoromycot<strong>in</strong>a, Mucoromycot<strong>in</strong>a, Mortierellomycot<strong>in</strong>a,Kickxellomycot<strong>in</strong>a and Zoopagomycot<strong>in</strong>a [1, 2]. The former two subphylaconta<strong>in</strong> species, which are human pathogenic caus<strong>in</strong>g <strong>in</strong>fections withdiverse predisposition and etiologies. They encompass ubiquitouslydistributed saprotrophic soil- or dead plant material-<strong>in</strong>habit<strong>in</strong>g fungi of theorder Mucorales (subphyl.: Mucoromycot<strong>in</strong>a, formerly classified <strong>in</strong>to thepolyphyletic class Zygomycetes). Human pathogenic species <strong>in</strong>habitdifferent growth temperature optima rang<strong>in</strong>g from 33 °C to 42 °C, whileattenuated species and stra<strong>in</strong>s exhibit lower temperature optima. Virulencewas tested <strong>in</strong> an embryonated hen egg model. S<strong>in</strong>gle and comb<strong>in</strong>edgenealogies based on distance, maximum parsimony, maximum likelihoodand Bayesian analyses of aligned nucleotide sequences of the nuclearencodedgenes for act<strong>in</strong> (act) and for the 5.8S ribosomal RNA flanked bythe <strong>in</strong>ternal transcribed spacer (ITS) regions 1 and 2 of a total of 150species were reconstructed. The phylogenetic reconstructions suggestmultiple orig<strong>in</strong>s of pathogenicity <strong>in</strong> certa<strong>in</strong> evolutionary l<strong>in</strong>eages. Forexample, four dist<strong>in</strong>ct families, Cunn<strong>in</strong>ghamellaceae, Lichtheimiaceae,Mucoraceae and Syncephalastraceae are <strong>in</strong>volved <strong>in</strong> disease developmentwith<strong>in</strong> the Mucoromycot<strong>in</strong>a [3-9]. Evolutionary trends are discussed withrespect to ecology, physiology and virulence.1. D.S. Hibbett, M. B<strong>in</strong>der, J.F. Bischoff. et al., Mycol. Res.111(2007), p. 509-547.2. K. Hoffmann, K. Voigt and P.M. Kirk, Mycotaxon115(2011), p. 353-363.3. A. Alastruey-Izquierdo, K. Hoffmann, G.S. de Hoog et al., J. Cl<strong>in</strong>. Microbiol.48(2010): 2154-2170.4. R. Vitale, G.S. de Hoog, P. Schwarz et al., J. Cl<strong>in</strong>. Microbiol. (2011), <strong>in</strong> press.5. W. Schrödl, T. Heydel, V.U. Schwartze et al., J. Cl<strong>in</strong>. Microbiol. (2011), <strong>in</strong> press.6. C. Schoch, K.A. Seifert, S. Huhndorf et al., PNAS (2011), <strong>in</strong> press.7. I. Ebersberger, R. de Matos Simoes, A. Kupczok et al. Mol. Biol. Evol (2011), <strong>in</strong> press.8. K. Voigt <strong>in</strong> “Syllabus of Plant Families”, ed. W. Frey et al., (Bornträger Verlag) (<strong>2012</strong>), <strong>in</strong> press.9. K. Voigt and P.M. Kirk <strong>in</strong> „Encyclopedia of Food Microbiology“, 2 nd ed., (Elsevier) (<strong>2012</strong>), <strong>in</strong> press.10. We k<strong>in</strong>dly acknowledge the Fungal Work<strong>in</strong>g Group of the International Fungal Barcod<strong>in</strong>g Consortiumand the Assembl<strong>in</strong>g the Fungal Tree of Life Consortium for <strong>in</strong>tegration <strong>in</strong>to their global network. We thankIngo Ebersberger (CIBIV, University of Vienna, Austria), Rytas Vilgalys and Andrij Gryganski (DukeUniversity Durham, NC, USA) and Conrad Schoch (NCBI, NIH, Bethesda, Maryland, USA) for stra<strong>in</strong> anddata share.BIOspektrum | Tagungsband <strong>2012</strong>
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Instruments that are music to your
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General Information2012 Annual Conf
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SPONSORS & EXHIBITORS9Sponsoren und
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11BIOspektrum | Tagungsband 2012
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13BIOspektrum | Tagungsband 2012
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22 AUS DEN FACHGRUPPEN DER VAAMMitg
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24 INSTITUTSPORTRAITin the differen
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26 INSTITUTSPORTRAITProf. Dr. Lutz
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28 CONFERENCE PROGRAMME | OVERVIEWS
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30 CONFERENCE PROGRAMME | OVERVIEWT
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42 SHORT LECTURESMonday, March 19,
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52ISV01Die verborgene Welt der Bakt
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54protein is reversibly uridylylate
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56that this trapping depends on the
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58Here, multiple parameters were an
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60BDP016The paryphoplasm of Plancto
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- Page 98 and 99: 98MEP025Regulation of pristinamycin
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- Page 142 and 143: 142bacteria in situ, we used 16S rR
- Page 144 and 145: 144bacteria were resistant to acid,
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- Page 152 and 153: 152OTP065The role of GvpM in gas ve
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162Streptomyces sp. strain FLA show
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164The study results indicated that
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166have shown direct evidences, for
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168biosurfactant. The putative lipo
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170the absence of legally mandated
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172where lowest concentrations were
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174PSV008Physiological effects of d
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176of pH i in vivo using the pH sen
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178PSP010Crystal structure of the e
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180PSP018Screening for genes of Sta
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182In order to overproduce all enzy
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184substrate specific expression of
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186potential active site region. We
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188PSP054Elucidation of the tetrach
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190family, but only one of these, t
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192network stabilizes the reactive
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194conditions tested. Its 2D struct
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196down of RSs2430 influences the e
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198demonstrating its suitability as
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200RSP025The pH-responsive transcri
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202attracted the attention of molec
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204A (CoA)-thioester intermediates.
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206Ser46~P complex. Additionally, B
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208threat to the health of reefs wo
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210their ectosymbionts to varying s
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212SMV008Methanol Consumption by Me
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214determined as a function of the
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216Funding by BMWi (AiF project no.
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218broad distribution in nature, oc
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220SMP027Contrasting assimilators o
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222growing all over the North, Cent
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224SMP044RNase J and RNase E in Sin
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226labelled hydrocarbons or potenti
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228SSV009Mathematical modelling of
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230SSP006Initial proteome analysis
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232nine putative PHB depolymerases
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234[1991]. We were able to demonstr
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236of these proteins are putative m
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238YEV2-FGMechanistic insight into
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240 AUTORENAbdel-Mageed, W.Achstett
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242 AUTORENFarajkhah, H.HMP002Faral
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244 AUTORENJung, Kr.Jung, P.Junge,
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246 AUTORENNajafi, F.MEP007Naji, S.
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249van Dijk, G.van Engelen, E.van H
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251Eckhard Boles von der Universit
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253Anna-Katharina Wagner: Regulatio
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255Vera Bockemühl: Produktioneiner
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257Meike Ammon: Analyse der subzell
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springer-spektrum.deDas große neue