VAAM-Jahrestagung 2012 18.–21. März in Tübingen

182In order to overproduce all enzymes from the reductive pathway in IBUN158B, the 1,3-PD operon of IBUN 13A was cloned into the backbone ofthe conjugational vector pMTL007C-E2 (Heap et al., 2010) under thecontrol of the P fdx promoter. The resulting plasmid can be conjugated ininactivation mutants of IBUN 158B with a protocol based on Cai et al.(2011) to improve their 1,3-PD yield.Cai G., Jin B., Saint C. et al., 2011. J. Biotechnol., 155, 269-274.Franz S., Montoya J., Montoya D. et al., 2011. BioSpektrum, Tagungsband zur VAAM-Jahrestagung 2011,155.Heap J., Kuehne S., Ehsaan M. et al., 2010. J. Microbiol. Methods, 80, 49-55.PSP028Molecular genetic examination of 1-(4-hydroxyphenyl)-ethanoloxidation in “Aromatoleum aromaticum” EbN1I. Büsing* 1,2 , L. Wöhlbrand 1 , R. Rabus 1,21 Institute for Chemistry and Biology of the Marine Environment (ICBM),General and Molecular Microbiology, Oldenburg, Germany2 Max Planck Institute for Marine Microbiology, Microbiology, Bremen,GermanyThe anaerobic degradation pathway forp-ethylphenol via 1-(4-hydroxyphenyl)-ethanol and p-hydroxyacetophenone in “Aromatoleumaromaticum” EbN1 was recently proposed, based on specific metaboliteand protein formation. Genes, encoding respective enzyme candidates,form a large operon-like structure on the chromosome of strain EbN1.Remarkably, this gene cluster contains two neighbouring genes fordehydrogenases, which may perform the oxidation of 1-(4-hydroxyphenyl)-ethanol to p-hydroxyacetophenone. Both dehydrogenasesshare similar identity (0.3 vs. mutant: OD max