VAAM-Jahrestagung 2012 18.–21. März in Tübingen

86DNA was extracted and shotgun sequenced using the SOLiD technique tostudy the composition of the intestinal microbiota and the existingmicrobial metabolism. By analyzing the data using BLASTX against theNR database and MEGAN we found that the microbiota composition of16S rRNA gene sequences obtained using Sanger sequencing and SOLiDsequencing provide comparable results. However, with SOLiD sequenceswe obtained more resolution down to the species level. In addition, withthe shotgun data we are able to identify the functional profile using SEEDand KEGG.This study shows that SOLiD mate-pair sequencing is a viable and costefficient option for analyzing a complex microbiome. To the best of ourknowledge, this is the first time that SOLiD sequencing has been used insuch a study.HMV004Bacteriocin production of staphylococcal nasal isolatesD. Janek*, B. Krismer, A. PeschelUniklinik Tübingen, Medizinische Mikrobiologie, Tübingen, GermanyStaphylococcus aureus is a major pathogen in hospital- and communityacquiredinfections. Colonisation of the anterior nares in about 30% of thepopulation is a major risk factor for S. aureus infections. Recently thecomposition of the nasal flora has been inverstigated. Interestingly, thebacterial diversity in the human nose reaches from aerobic to strictlyanaerobic bacteria. The most frequently occurring species areCorynebacterium accolens/ C. macginleyi, S. epidermidis andPropionibacterium acnes. In order to investigate if bacteriocin productionmight play a role during nasal colonisation, we analysed the bacteriocinproduction of nasal Staphylococcus strains.The test-strains were casted in an agar plate and the nasal isolates werestamped on the plate. Various isolates showed growth inhibition zones ofthe test-strains. Transposon plasmids could be transformed into variousstrains and mutagenesis was performed.Analysis of 93 stapylococcal nasal isolates offered that various strainsproduce bacteriocins against Micrococcus luteus and other nasal bacteria(S. aureus, Corynebacteria, Moraxella, Propionibacteria…).Thebacteriocin production of some nasal isolates turned out to be inducible byhydrogen peroxide or iron limitation.One of these bacteriocins, produced by an S. epidermidis strain, could becharacterized as a Nukacin-like lantibiotic with activity againstMicrococcos luteus, Moraxella catarrhalis, Streptococcus pyogenes andCorynebacterium pseudodiphtheriticum.Knowledge about the various interactions between staphylococcal andother nasal isolates could be important for effective S. aureus controlstrategies.HMP001Microbiological air quality in the hospital environments of twomajor hospitals in Benin City metropolis, Edo State, NigeriaF.O. Ekhaise*University of Benin, Microbiology, Benin, NigeriaWe spend most of our lives in different indoor environments, in homes,day-care facilities, schools and workplaces and we are constantly beingchallenged by the microbial contents of these environments. It becameimperative to undertake a study of the microbiological air quality of theairborne microflora in the environments of two major government ownedhospitals (University of Benin Teaching Hospital, (UBTH) and CentralHospital) in Benin City metropolis. The air samples were sampled everymonth for the three (3) months in the wet season (June - August, 2010) andthree (3) months of the dry season (November 2010 - January 2011) usingthe settled plate methods. The study sites were divided into nine unitswhich includes Accident and Emergency Ward, Laboratory, Male ward,Female Ward, Children Ward, Labour Room, Treatment Room, Theatreand outside the hospital gate. The mean airborne bacterial load in the twohospitals ranges from 8.5cfu/min to 172.5cfu/min and 5.5cfu/min to64.5cfu/min for UBTH and Central hospital in the wet season. While themean airborne fungal load in UBTH and Central hospital in dry seasonranges from 2.5cfu/min to 9.5cfu/min and 1.5cfu/min to 19.0cfu/minrespectively. The female ward, children ward, accident and emergencyward and outside the hospital gate were recognized to record the highestairborne microflora. The result revealed the isolation of ten (10) fungalairborne isolates and six (6) airborne bacterial isolates. These includes,Aspergillus niger, Aspergillus flavus, Botryodiplodia acerina, Rhizopusstolonifer, Nigospora zimm, Mucor sp., Monilla infuscans, Penicillium sp.,Candida sp. and Trichoderma viridis, while the six (6) bacterial isolatesincludes Staphylococcus aureus, Staphylococcus epidermidis, Bacilluscereus, Bacillus sp., Serratia marcescens and Micrococcus sp. The resultshows the highest fungal population of 26.5cfu/min (Outside environment)in UBTH followed by 24.0cfu/min (Outside environment) in CentralHospital. The highest bacterial population of 172.5cfu/min (outsideenvironment) was recorded in UBTH. The fungal isolates Aspergillusniger (53.0%) and Monilla infuscans (43.9%) were showed to be the mostfrequently isolated airborne fungal isolates while Staphylococcus aureus(91.3%) and Staphylococcus epidermidis (85.8%) were the mostfrequently isolated airborne bacterial isolates. The Statistical analysisshowed no significant difference between the values obtained during thewet and dry seasons in both hospitals studied. KEYWORD: Airbornemicroflora, bacteria, fungi, hospital environment, time and bioaerosols.HMP002Unusual Multi organ presentation of Hydatid cystY. Hashemi Aghdam* 1 , V. Montazeri 2 , R. Azhough 2 , H. Farajkhah 2 ,*A. Moradi 2 , M. Naghavi Behzad 3 , S. Rahimi 4 , *F. golchin 41 Islamic Azad University, Medical Faculty, Young Researchers Club, TabrizBranch, Tabriz, Islamic Republic of Iran2 Tabriz University of Medical Sciences, Medical Faculty, Tabriz, IslamicRepublic of Iran3 Tabriz University of Medical Sciences, Medical Faculty, Student ResearchCommittee, Tabriz, Islamic Republic of Iran4 Islamic Azad University, Medical Faculty, Tabriz, Islamic Republic of IranIntroduction: Hydatidosis is a common problem all over the world.Hydatid cysts could be formed in all parts of human body except hair andnail that there is no blood. The prevalence of this disease is higher inchildren rather than adults. Risk of infection depends on sanitation and itcould be prevented easily. The ultimate treatment is surgery but recurrentrate can be decreased by administering medical treatment in thepreoperative and post operative periods.Case Report: A 35 year's old female patient presented with cough,purulent production and dyspnea. In computerized tomographic scan (CTscan) numerous cysts were observed in chest, abdomen and paravertebralmuscles. Because of cysts were ruptured, surgical intervention wasplanned for thoracic lesions without prior antiparasitic medical treatment.The patient had no complications in short- term follow up.Discussion: Hydatid cyst is a parasitic disease which is known from thetime of Hippocrates. Infection will occur by eating vegetablescontaminated with eggs of this parasite or contaminated viscera ofherbivores and parasite larvae form cyst in human body. The infectedperson is in risk of pneumocysis, pleural effusion, pneumothorax,secondary Echinococcus in pleural and peritoneal cavities and musclesinvolvement. In our case there was platysma Dorsey and abdominalmuscle involvement that there was no problem despite of dorsal andpleural cysts rupture, fortunately. Another interesting point in this case wasthat, there was no relation between pleural and dorsal muscle cysts.Generally observance of basic principles of health such as washing handswith soap after gardening or contact with dogs and also washingvegetables that could be contaminated with dog feces are very importantpoints preventing these diseases. However this disease could be curedeasily by surgery, if surgery is conducted after taking a short course ofAlbendazole and Mebendazole, the efficacy of surgical treatment will bebetter.HMP003Relationship among chlamydia pneumoniae infection,atherosclerosis and expectancy of coronary artery diseaseY. Hashemi Aghdam* 1 , S. Rahimi 2 , A. Moradi* 3 , R. Ghassemi Nezhad* 4 ,F. Gholchin* 2 , M. Naghavi Behzad 51 Islamic Azad University, Medical Faculty, Young Researchers Club, TabrizBranch, Tabriz, Islamic Republic of Iran2 Islamic Azad University, Medical Faculty, Tabriz, Islamic Republic of Iran3 Tabriz University of Medical Sciences, Medical Faculty, Tabriz, IslamicRepublic of Iran4 Student of Azerbaijan Medical University, Dentistry Faculty, Baku, IslamicRepublic of Iran5 Tabriz University of Medical Sciences, Medical Faculty, Student ResearchCommittee, Tabriz, Islamic Republic of IranIntroduction: Coronary artery disease (CAD) is the leading cause of deathin many countries. The underlying mechanism of the chronic inflammatoryprocess in atherosclerosis is still unknown (1). But, the risk for coronaryevents may rise during acute infection (2). There are findings suggestingthe inflammatory and immunogenic nature of the atherosclerosis (3). It iscurrently unclear what causes the chronic inflammation withinatherosclerotic plaques. One emerging paradigm suggests that infectionwith bacteria and/or viruses can contribute to the pathogenesis ofatherosclerosis (4). Chronic Chlamydia pneumoniea infection has recentlybeen associated with atherosclerosis. The aim of this study was toinvestigate the association of Chlamydia pneumoniea infection, Ischemicheart disease (IHD) and atherosclerosis.Material and Method: 86 patients with background of recent IschemicHeart Disease, referred to Shahid Madani hospital of Tabriz from January2010- January 2011 were studied. Different blood samples were taken toassess the lipid profile and other tests. Blood culture was done andTriglyceride, High-Density Lipoprotein (HDL), Low density Lipoprotein(LDL), IgG and IgA antibodies against Chlamydia Pneumoniea wereBIOspektrum | Tagungsband 2012