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Windows QTL Cartographer 2.5 - FTP Directory Listing

Windows QTL Cartographer 2.5 - FTP Directory Listing

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<strong>Windows</strong> <strong>QTL</strong> <strong>Cartographer</strong> <strong>2.5</strong><br />

Import data - MapMaker format<br />

1. Start Win<strong>QTL</strong>Cart and select menu item File>Import or click .<br />

2. Select Mapmaker/<strong>QTL</strong> format and click Next button.<br />

3. Click button Map File to select file "mapmaker-samp.map".<br />

4. Click button Cross Data to select file "mapmaker-samp".<br />

5. Enter file name "mapmaker-samp_In" in edit box and click button Finish.<br />

Import data - Excel format<br />

1. Start Win<strong>QTL</strong>Cart and select menu item File>Import or click .<br />

2. Select Microsoft Excel format and click Next button.<br />

3. Click button Excel File to select file "wqcart-samp.xls".<br />

4. Enter file name "wqcart-samp_xls_In" in edit box and click button Finish.<br />

Import data - CSV format<br />

1. Start Win<strong>QTL</strong>Cart and select menu item File>Import or click .<br />

2. Select Microsoft CSV format and click Next button.<br />

3. Click button CSV File to select file "NSimuB1-01-OnePop.csv".<br />

4. Enter file name "NSimuB1-01-OnePop_in" in edit box and click button Finish.<br />

Simulation source data file<br />

1. Start Win<strong>QTL</strong>Cart and select menu item File>Simulation or click .<br />

2. In step 1/3, set parameters as:<br />

Sample size: 200<br />

Trait mean: 15.85<br />

Random seed: any<br />

Map function: Haldane<br />

Cross type: B1<br />

Translation table: AA>2, Aa>1, A>*<br />

Total chromosome number: 3<br />

3. Set The current chromosome as 1, Markers for chromosome 1 as 15, Average space<br />

between marker as 9, Variations of the marker position as 10%. Click button Set as<br />

default for all chromosomes to set parameter for other chromosome. Click button Next to step<br />

2/3 dialog window.<br />

4. In step 2/3, set parameters as:<br />

<strong>QTL</strong> numbers: 7<br />

Heritability: 0.82<br />

Additive effect: Effects direction>Same<br />

Effects distribution>Normal<br />

Epistatic effect: None by uncheck the check box<br />

Filename: "Nsimu-01"<br />

Click button OK to step 3/3 dialog window.<br />

5. In step 3/3, Click button Adjustment to adjust all parameters. Click button Save to save the<br />

parameter setting to file "Nsimu-01.qpe". Click button OK to finish.<br />

© 2010 N.C. State University, Bioinformatics Research Center

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