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Windows QTL Cartographer 2.5 - FTP Directory Listing

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<strong>Windows</strong> <strong>QTL</strong> <strong>Cartographer</strong> <strong>2.5</strong><br />

Interval mapping<br />

1. Select Menu Item File>Open or click to open source data file "mouse.mcd".<br />

2. Select Menu Item Method>Interval Mapping or click to open the Form of IM analysis.<br />

3. Select By Permutation and click button OK to start threshold value calculation.<br />

4. Click button Start to start IM analysis.<br />

5. Click "Y" to show <strong>QTL</strong> summary in Text Window.<br />

Composite interval mapping<br />

1. Select Menu Item File>Open or click to open source data file "wqcart-samp0.mcd". You<br />

can just click the tree item if the file has already been opened.<br />

2. Select Menu Item Method>Composite Interval Mapping or click to open the Form of<br />

CIM.<br />

3. Click button Control to set the parameter. Set Control marker number as 8, Window size as<br />

15.0, Other trait as 1,2 and click button OK to back.<br />

4. Select All traits in trait selection pull-down menu.<br />

5. Click button Start to begin CIM analysis.<br />

Multiple-trait analysis<br />

1. Select Menu Item File>Open or click to open source data file "wqcart-samp0.mcd".<br />

2. Select Menu Item Method>Multiple Traits Analysis.<br />

3. Select IM Method and input 1,3 in edit box for Multiple Traits Setting Dialog.<br />

4. Click button Start to begin multiple traits analysis.<br />

Multiple Interval Mapping<br />

1. Select Menu Item File>Open or click to open source data file "wqcart-samp0.mcd".<br />

2. Select Menu Item Method>Multiple Interval Mapping or click and select trait MECIAIA to<br />

enter the Form of MIM.<br />

3. Click button New Model, click "Y", and click button OK to obtain an initial MIM model.<br />

4. Click button Refine Model. Select Optimizing position and click button OK.<br />

5. Click button Start to start <strong>QTL</strong> position optimization procedure.<br />

6. Click button Save Model to save the <strong>QTL</strong> model.<br />

7. Click button Refine Model. Select Searching for new <strong>QTL</strong>s and click button OK.<br />

8. Select Search for <strong>QTL</strong>s and click button Start for searching for more <strong>QTL</strong>.<br />

9. Click button Refine Model and optimize <strong>QTL</strong> position again.<br />

10. Click button Test All to see LR values for each <strong>QTL</strong> in Text window.<br />

11. Click button Save Model to save the <strong>QTL</strong> model again. You may change filename if you want to<br />

keep the previous <strong>QTL</strong> model.<br />

© 2010 N.C. State University, Bioinformatics Research Center

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