Windows QTL Cartographer 2.5 - FTP Directory Listing
Windows QTL Cartographer 2.5 - FTP Directory Listing
Windows QTL Cartographer 2.5 - FTP Directory Listing
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Create new source data file<br />
© 2010 N.C. State University, Bioinformatics Research Center<br />
Tutorials 83<br />
1. Start Win<strong>QTL</strong>Cart and select menu item File>New or click .<br />
2. Set parameters in step 1 of 2 as:<br />
Chromosome number: 2<br />
Trait number: 2<br />
Other trait number: 1<br />
Individual number: 125<br />
Symbol of missing trait: .<br />
Crossing type: B1<br />
File stem name: Test<br />
AA 2<br />
Aa 1<br />
Aa *<br />
3. Click OK to step 2 of 2.<br />
4. Click button Notepad to open file "Text.txt" in default directory (C:\NCSU\Win<strong>QTL</strong>Cart).<br />
5. In Notepad, select chromosome label (CHROM-1, CHROM-2) and copy to <strong>Windows</strong> clipboard.<br />
Minimize Notepad.<br />
6. Back to Create New Source File – Step 2 0f 2. Click button Paste to show chromosome labels<br />
in Edit Window. Select Chromosome Labels in Data Type Selection. Click button Send<br />
Data to input chromosome labels.<br />
7. Select and copy Marker Number in Notepad. Click button Paste, click Marker numbers in<br />
Data Type Selection and click button Send Data to input marker numbers.<br />
8. Select and copy Marker Label and Position in Notepad. Click button Paste, Select Marker<br />
positions in Data Type Selection and click button Send Data. Answer "Y" in the dialog to<br />
input marker labels and positions.<br />
9. Select and copy Marker Genotype and paste. Click Marker genotypes and click button<br />
Send Data . Answer "Y" to input marker genotype data.<br />
10. Select, copy and Paste Trait Value. Select Trait Values and click button Send Data. Answer<br />
"Y" to input trait values.<br />
11. Select, copy and Paste Other Trait Value. Select OTrait Values and click button Send Data.<br />
Answer "Y" to input other trait values.<br />
12. Click button OK to create source data file (Test.mcd).<br />
Single marker analysis<br />
1. Select Menu Item File>Open or click to open source data file "wqcart-samp0.mcd".<br />
2. Select Menu Item Method>Single Marker Analysis to open the Form of single analysis.<br />
3. Use the poll-down menu to select a trait. Click button Result to show information of single<br />
marker analysis or statistical summary.<br />
4. Click button Graph File to produce result file and show in graph dialog.<br />
5. Click button OK to finish single marker analysis.
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