11.08.2013 Views

Windows QTL Cartographer 2.5 - FTP Directory Listing

Windows QTL Cartographer 2.5 - FTP Directory Listing

Windows QTL Cartographer 2.5 - FTP Directory Listing

SHOW MORE
SHOW LESS

You also want an ePaper? Increase the reach of your titles

YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.

© 2010 N.C. State University, Bioinformatics Research Center<br />

Tutorials 85<br />

12. Click button Refine Model. Select Searching for new <strong>QTL</strong>s and click button OK. Select<br />

Search for Epistasis and click button Start for searching for <strong>QTL</strong> interact (epistasis). Answer "Y"<br />

to add the epistatic effect.<br />

13. click button Refine Model. Select MIM Model Summary and click button OK.<br />

14. Select Graphic Result File and click Start to produce result file and show it in graph dialog.<br />

15. click button Refine Model. Select MIM Model Summary and click button OK. Select Model<br />

Summary File and click Start to produce MIM summary information.<br />

16. In tree pane, you can click to show information of breed value, <strong>QTL</strong> information, R2 partition, and<br />

variance and co-variance table.<br />

17. Click button Finish and button Save to finish the MIM session.<br />

Bayesian interval mapping<br />

1. Select Menu Item File>Open or click to open source data file "wqcart-samp0.mcd".<br />

2. Select Menu Item Method>Bayesian Interval Mapping to enter the Form of BIM.<br />

3. You can click button Parameter to set the BIM parameters.<br />

4. Click button Start to begin CIM analysis.<br />

Result manipulation<br />

1. Start Win<strong>QTL</strong>Cart and select menu item View>Visualize Result or click to open result<br />

file "wqcart-samp0-C.qrt".<br />

2. Use menu item Chrom to select and show one chromosome, all chromosomes, and any number<br />

of chromosomes with different order.<br />

3. Use menu item Traits to select and show any number of traits.<br />

4. Select menu item Tools>Display one page format or click to show graph in different<br />

format.<br />

5. Select menu item Setting>set display parameters or click to adjust parameter of graph.<br />

6. Select menu item Tools>Show <strong>QTL</strong> information or click to obtain the <strong>QTL</strong> summary<br />

information from the graph or open a <strong>QTL</strong> summary file (*.qtl).<br />

7. you can trace on graph by selecting menu item Setting>Trace coordinate in graph or click<br />

. Double click to show the coordinate numbers in graph.<br />

8. You can combine two graphs into one by selecting menu item File>Add <strong>QTL</strong> result file.<br />

Technical notes<br />

Troubleshooting<br />

1. Errors even to run Single Marker Analysis<br />

Win<strong>QTL</strong>Cart has problem to deal with complex folder and file name. To avoid this problem, please try put<br />

your MCD file in a simple folder such \NCSU\Work1\ and only letter and digital are in file name.

Hooray! Your file is uploaded and ready to be published.

Saved successfully!

Ooh no, something went wrong!