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Physiology and Molecular Biology of Stress ... - KHAM PHA MOI

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268<br />

B. Rathinasabapathi <strong>and</strong> R. Kaur<br />

3.1.7. Polyamines<br />

In rice <strong>and</strong> wheat, it has been found that polyamine accumulation affects drought <strong>and</strong><br />

salinity tolerance <strong>of</strong> the plant. Transgenic plants engineered with a chimeric gene for<br />

Datura arginine decarboxylase showed increase in polyamine levels <strong>and</strong> ultimately,<br />

displayed 5-fold salt tolerance as compared to wild-type plants, <strong>and</strong> were capable <strong>of</strong><br />

surviving <strong>and</strong> performing well for 20 d without water, whereas wild-type plants started<br />

deterioration if water was withheld for more than 4 d (Capell et. al., 2002; Capell, 2004).<br />

Figure 4. In the members <strong>of</strong> the Plumbaginaceae, beta-alanine is shared<br />

between the synthesis <strong>of</strong> osmoprotectant beta-alanine betaine <strong>and</strong> pantothenate.<br />

NMT is a trifunctional beta-alanine N-methyltransferase (Raman <strong>and</strong><br />

Rathinasabapathi, 2003) <strong>and</strong> PS is pantothenate synthetase (Genschel et. al.,<br />

1999), both presumably cytosolic enzymes<br />

3.1.8. Ectoine<br />

Ectoine is an osmoprotectant found in Halomonas elongata <strong>and</strong> other halophilic bacteria.<br />

Aspartate semialdehyde is the precursor <strong>of</strong> ectoine (Ono et. al., 1999). Nakayama<br />

et. al., (2000) engineered tobacco with the three H. elongata genes (ectA, ectB, <strong>and</strong><br />

ectC) encoding the enzymes responsible for ectoine synthesis. Transgenic cells were<br />

characterized by the production <strong>of</strong> low levels <strong>of</strong> ectoine (

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