Physiology and Molecular Biology of Stress ... - KHAM PHA MOI

Functional Genomics of Stress Tolerance
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Table 2. List of some genes in the stress signaling pathway identified through insertional
mutagenesis by T-DNA
Gene Stress Function Reference
AtHKT1
(Arabidopsis thaliana Salt Controls sodium Rus et al. (2001)
high affinity K + tranporter)
entry into plant roots
*AtCPL1 (Arabidopsis Cold, ABA, Negative regulator Koiwa et al.
thaliana C-terminal salt of stress gene (2002)
domain phosphatase-like)
transcription
*AtCPL3 (Arabidopsis ABA Negative regulator Koiwa et al.
thaliana C-terminal of stress gene (2002)
domain phosphatase-like)
transcription
*OSM1/SYP61 ( Oryza Salt, drought, Stomatal response to Zhu et al. (2002)
sativa syntaxin) ABA ABA
CBL1 (Arabidopsis Cold, salt, Positive regulator of Cheong et al.
thaliana calcineurin drought drought and salt and (2003)
B-like protein)
negative regulator of
cold stress
CIPK3 (Arabidopsis ABA, cold, Cross talk between Kim et al. (2003)
thaliana calcium salt cold and ABA signal
associated protein kinase)
transduction
OsRLK1 (Oryza sativa cold Cold response Lee et al. (2004)
putative LRR-type
receptor-like–protein
kinase)
OsDMKT (Oryza sativa cold Cold response Lee et al. (2004)
putative demethyl
menaquinone methyl
transferase)
* Mutants generated in RD29A:LUC background
1999) and one T-DNA tagged collection (Bouchez et al., 1993) were used. A total of 47
insertions were identified in 36 members of the R2R3 MYB family. Screening for an
altered phenotype in 32 lines representing disruptions in 26 MYB genes showed no
distinct phenotype in most of the lines analyzed using screens for stress among others
with the exception of a UVB stress tolerant line (Meissner et al., 1999).
A modified approach to the use of T-DNA and transposon tagging for insertional
mutagenesis is the use of enhancer elements in the construct. These enhancers