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Proceedings of the Fifth Asian Regional Maize Workshop - Search ...

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Recycling <strong>of</strong> good lines also can be practiced to develop new lines. Recycling is <strong>of</strong>ten<br />

done between lines from popu1ation(s) within <strong>the</strong> same heterotic group. There are no set guidelines<br />

for <strong>the</strong> recycling <strong>of</strong> lines. Much depends also on how a breeder is handling his germplasm<br />

and what kinds <strong>of</strong> difficulty(ies) he is encountering in developing good lines. From ongoing<br />

population improvement programs involving S1 or S2 as a procedure or as a step in <strong>the</strong> breeding<br />

process, one can always resort to recycling <strong>of</strong> early generation lines. Also, for<br />

germp1asm/materia1s known to be notably poor for line development, it would be advisable to reinitiate<br />

recycling <strong>of</strong> early generations to increase <strong>the</strong> possibility <strong>of</strong> developing good lines.<br />

This will be a good strategy at least in <strong>the</strong> early maturing germp1asm where inbreds with high<br />

degree <strong>of</strong> inbreeding are much more difficult to.obtain. Information on combining ability <strong>of</strong><br />

early generation lines is <strong>of</strong>ten obtained from <strong>the</strong> ongoing population improvement programs.<br />

Systematic com~ining ability tests are <strong>of</strong>ten conducted at S4 or past S4 stage. By this time one<br />

will have a fairly good idea as to whe<strong>the</strong>r or not <strong>the</strong> line is usable. Promising lines are also<br />

evaluated in line evaluation trials (LETs). Lines in <strong>the</strong>se trials are characterized more<br />

thoroughly and sometimes one or two replications are planted under high plant density and<br />

artificial disease stress conditions. Data collected in <strong>the</strong>se trials is <strong>of</strong> great help in<br />

planning crossing program to develop single and three-way crosses.<br />

8. Development <strong>of</strong> non-inbred progenitors.<br />

Non-inbred progenitors or materials <strong>of</strong> an OPV can be used in developing non-conventional<br />

maize hybrids. Most breeders in <strong>the</strong> past have used bulks <strong>of</strong> populations and varieties as<br />

parent(s) <strong>of</strong> a non-conventional hybrid. As one would expect, if <strong>the</strong> parents are variable, <strong>the</strong><br />

hybrids would also be veriable. To obtain uniformity and appeal in such hybrids, it is <strong>the</strong>refore<br />

quite important that such non-inbred progenitors should be created specifically for <strong>the</strong> purpose<br />

<strong>of</strong> using parents <strong>of</strong> a non-conventional hybrid. It will be preferable to use experimental<br />

varieties, elite line syn<strong>the</strong>tics, and narrow genetic base syn<strong>the</strong>tics which have been reconstituted<br />

using only a few relatively uniform families and lines. There are great opportunities for<br />

developing such progenitors from <strong>the</strong> ongoing intrapopu1ation and interpopulation improvement<br />

programs. Interpopulation improvement programs permit development <strong>of</strong> cross-performance syn<strong>the</strong>tics<br />

using test cross data to maximize heterosis manifestation in intersyn<strong>the</strong>tic hybrids.<br />

9. Identification <strong>of</strong> testers.<br />

Testers, inbreds as well as non-inbreds, serve exceedingly useful functions during various<br />

phases <strong>of</strong> population and hybrid breeding programs. Non-inbred testers for population improvement<br />

programs, evaluation <strong>of</strong> combining ability, and when used as a parent <strong>of</strong> a hybrid, should be<br />

developed with some resource effort. These testers could be <strong>of</strong> narrow genetic base involving a<br />

few lines or families and can be reconstituted using data from population improvement programs,<br />

topcross tests, and from any o<strong>the</strong>r data which permits forming such progenitors. Perhaps, it may<br />

also be more appropriate to develop such non-inbred testers using various groups/pairs <strong>of</strong><br />

heterotic population undergoing improvement or use in hybrid breeding program.<br />

Identification <strong>of</strong> inbred testers will be <strong>of</strong> immense help in any hybrid breeding program.<br />

In fact it is a priority item to identify a set <strong>of</strong> testers which will serve as a reference point<br />

for meeting various objectives in a hybrid breeding program. Unfortunately, such tester lines<br />

are not freely available, if at all <strong>the</strong>y exist, to maize scientists <strong>of</strong> <strong>the</strong> developing countries<br />

for <strong>the</strong> lowland tropical and subtropical germp1asm. Such tester lines could be identified using<br />

special mating designs such as dia11e1s and design-2. Once such first-set <strong>of</strong> testers are<br />

identified, <strong>the</strong>y can be continuously replaced by new and superior lines resulting from <strong>the</strong> same<br />

heterotic group. Though CIMMYT's effort in. hybrid development was initiated with a modest<br />

effort, we made special effort to identify tester lines for different classes <strong>of</strong> germplasm.<br />

These lines have been successfully used in developing hybrid combinations and to sort out<br />

heterotic patterns <strong>of</strong> lines. Separation <strong>of</strong> lines by heterotic pattern(s) facilitates breeder's<br />

job in developing hybrid combinations and to form and/or to broaden <strong>the</strong> already known heterotic<br />

groups. Inbreds from <strong>the</strong> same heterotic group could also be used to develop and identify single<br />

cross testers. In <strong>the</strong> near future, CIMMYT will announce lines that can be used as tester by <strong>the</strong><br />

NARS and <strong>the</strong> private sector breeders.<br />

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