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Proceedings of the Fifth Asian Regional Maize Workshop - Search ...

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Production <strong>of</strong> ~zygous diploid inbreds through <strong>the</strong> haploidy rout•.<br />

Attempts are being made to develop a suitable technology for quick production <strong>of</strong> homozygous<br />

diploid lines <strong>of</strong> maize through <strong>the</strong> haploidy route. The haploids are being isolated through<br />

<strong>the</strong> genetic selection technique. Haploid-inducer lines with haploidy potential <strong>of</strong> 5X have been<br />

developed. The haploids are screened at <strong>the</strong> dry seed stage with <strong>the</strong> help <strong>of</strong> genetic markers. No<br />

standard technique is yet available for efficient doubling <strong>of</strong> <strong>the</strong> haploids. Two approaches have<br />

been followed, (i) colchicine treatment to germinating plumule <strong>of</strong> young seedlings and (ii)<br />

culturing haploid tissue and regeneration following doubling <strong>of</strong> chromosome nl.lllber. SO far, <strong>the</strong><br />

best success (18.5X doubling) has been obtained with treatment <strong>of</strong> germinating seeds (having <strong>the</strong><br />

plumule tip cut) with O.oex colchicine solution with dimethyl sulfoxide for 8 hr at 18C.<br />

Tab1e 7. Mean performance <strong>of</strong> experimenta1 hybrids at Hyderabad and Arbhavi. 1992 SlInmer.<br />

Experimental Yield (kg/ha) X over<br />

hybrids Hyd Arb check<br />

BH-44151 4376 8954 6665 31.4<br />

BH-44181 4725 8376 6550 29.2<br />

BH-44071 4669 7725 6202 22.3<br />

BH-44081 4342 8049 6195 22.2<br />

BH-43831 4724 7612 6168 21.6<br />

BH-44201 4721 7502 6111 20.5<br />

BH-43921 4280 7398 5839 15.1<br />

BH-44131 3255 8287 5771 13.8<br />

BH-44101 4489 7021 5755 13.5<br />

Trishu1ata (check) 4047 6096 5071<br />

Table 8. Mean performance <strong>of</strong> single cross hybrids at Hyderabad and Arbhavi. 1992-93 Winter.<br />

Experimental Yield (kg/ha) Mean X over<br />

hybrids Hyd Arb check<br />

BH-6055 8774 8488 8631 26.68<br />

BH-6060 8063 9149 8606 26.32<br />

BH-6066 7181 8798 7990 17.28<br />

BH-6063 6854 9007 7931 16.41<br />

BH-6084 7140 8596 786 15.49<br />

BH-6078 8074 7638 7856 15.31<br />

BH-6064 7315 8285 7800 14.49<br />

DI....t-105 (check) 7162 6464 6813<br />

Crop managellI8l"lt research.<br />

The agronomic investigations led us to conclude that during <strong>the</strong> summer season in <strong>the</strong><br />

plains <strong>of</strong> nor<strong>the</strong>rn India, <strong>the</strong> planting <strong>of</strong> maize may be taken up any time between last week <strong>of</strong> May<br />

to last week <strong>of</strong> June. For hi lly regions, fi rst week <strong>of</strong> April has been found to be most appropriate.<br />

To ensure optimum yield planting spacing <strong>of</strong> ei<strong>the</strong>r 75 x 20 em or 60 x 25 em along with a<br />

50

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