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Dames & Moore, 1999 - USDA Forest Service

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The invertebrate sampling was performed using a kick net of 500 micron mesh. The work<br />

performed by <strong>Dames</strong> & <strong>Moore</strong> used an enclosed sampler with 250 micron mesh netting<br />

and is considered a preferred sampling procedure. The invertebrates collected by Ecology<br />

did not represent the full range of invertebrates collected by <strong>Dames</strong> & <strong>Moore</strong> and<br />

demonstrates a lack of disturbance of the substrate when collecting.<br />

The taxa list, metals tolerance list, and mean number of organisms provided in the Ecology<br />

report show a nearly complete lack of invertebrate organisms at and downstream of the<br />

Site. The <strong>Dames</strong> & <strong>Moore</strong> data demonstrate a very different picture with a change in<br />

community structure and reduction in numbers.<br />

The invertebrates found by <strong>Dames</strong> & <strong>Moore</strong> at and downstream of the Site are among the<br />

most metal intolerant organisms found on the list provided by Ecology. It is not likely that<br />

the community changed significantly between 1996 (Ecology sampling) and 1997 (<strong>Dames</strong><br />

& <strong>Moore</strong> sampling) but the difference is more likely a result of the collection methods<br />

employed in the two studies.<br />

4.6.1.4 Fish Communities '<br />

Ecology found the sediment not toxic and the toxicity of water was uncertain.<br />

Fish communities within Railroad Creek consist of primarily cutthroat (Oncorhynchus clarki spp.) and<br />

rainbow trout (0. miss). Mottled sculpin (Cottus bairdt] have also been observed within Railroad Creek<br />

(PNL, 1992). Numerous fish barriers located within the canyon relief of reach B preclude the upstream<br />

movement of fish originating in lower Railroad Creek or Lake Chelan. Occasionally, Kokanee (0. nerh<br />

nerh) will enter the creek from Lake Chelan iri late September or early October in search of spawning<br />

areas. During the fall (1997) investigations, approximately 10 Kokanee salmon were observed in lower<br />

Railroad Creek. Although a comprehensive spawning survey was not included in the 1997 investigations,<br />

opportunistic observations were made of the stream substrate conditions during a reconnaissance of<br />

Railroad Creek in early September and during the subsequent benthic macroinvertebrate ad fish<br />

sampling. Upon observing Kokanee in the lower segment of Railroad Creek (within approximately 300<br />

yards of Lake Chelan), a more thorough reconnaissance of substrate conditions was conducted within that<br />

segment of Railroad Creek in an attempt to identify the existence of gravel suitable for spawning.<br />

Considering female salmon usually dig redds in a riffle area in small- to medium-size gravel (Wydoski<br />

and Whitney, 1979), the results of the reconnaissance indicated that very little, if any, substrate suitable<br />

for spawning existed within this segment of Railroad Creek.<br />

Fish communities were sampled by snorkeling at all ten locations while electrofishing was utilized at all<br />

locations excluding South Fork Agnes Creek and Company Creek. The results of the snorkeling and<br />

electrofishing sampling are presented in Table 4.6-3. A summary of the general composition and condition<br />

of the fish at each location electrofished is presented in Table 4.6-3a.<br />

As indicated during past fisheries studies of Railroad Creek (PNL, 1992), cutthroat trout were the most<br />

abundant trout collected throughout all locations while rainbows and rainbowlcutthroat hybrids were<br />

occasionally collected. Cutthroat trout were present at all ten sampling locations. Rainbow trout were<br />

G:\WPDATA\WS\REPORTS\HOLDEN.2WM-O.DOC<br />

17693-005-019Vuly 19. <strong>1999</strong>;4:5 1 PM:DRAFT FINAL RI REPORT

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