Smartline Autosampler 3900 Manual / Handbuch V 1508 03/2007
Smartline Autosampler 3900 Manual / Handbuch V 1508 03/2007
Smartline Autosampler 3900 Manual / Handbuch V 1508 03/2007
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Injection Principle 19<br />
For the first injection, after a wash or<br />
after emptying of the buffer tubing.<br />
The syringe dispenser aspirates<br />
transport liquids from the transport vial<br />
to fill the sample line with transport<br />
liquid and remove wash solvents.<br />
Fig. 18 µl pick-up injection: filling sample lines with transport liquid.<br />
The needle moves from the transport<br />
vial to the sample vial. The injection<br />
valve is switched into the LOAD<br />
position.<br />
Fig. 19 µl pick-up injection: go to sample vial and switch injection valve to LOAD.<br />
Fig. 20 µl pick-up injection: filling the sample loop.<br />
The programmed injection volume is<br />
aspirated from the sample vial.<br />
The sample needle moves back to the<br />
transport vial.<br />
The sample is quantitatively<br />
transported into the loop, with<br />
transport liquid (mobile phase) from<br />
the transport vial.<br />
Fig. 21 µl pick-up injection: transporting sample into the sample loop.<br />
The injection valve switches into the<br />
INJECT position. The sample loop is<br />
now part of the HPLC mobile phase<br />
flow path: sample is transported to the<br />
column. The analysis starts.
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