Smartline Autosampler 3900 Manual / Handbuch V 1508 03/2007

68 APPENDIX C: Test Procedure
Series 2 to 4
Method number: 2
Calibration vial: No
First sample vial: 01
Last sample vial: 02
Due to the fact that the eluent is injected from vial 2, only a very small
peak or no peak at all should be seen. A chromatogram as illustrated in
Fig. 53 will result.
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UV-Detektor[1]
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Fig. 53: Sample Carry-Over Test
Injection of Eluents
1 2 3 4 5 6 7 8 9 10 11 12 13 14
Minutes
The respective determined peak areas (the mean) for the eluent injection
are put into a ratio of the uracil peak areas. The sample carry-over
percent PV can be calculated with the following equation:
PV [%] X 100 (Sample carry-over)
The sample carry-over percent PV should equal 0.3%.
Record the results on the test report form.
3. Linearity
The system settings and analytical parameters are determined as
explained in test 1.
10, 20, 30, 40 und 50 µl of the uracil test solution are injected three times
each from vial 1. In order to do this 5 different methods with different
injection volumes must be programmed. The 5 methods are to be used in
series 5-9. Make sure that there is sufficient test solution in vial 1
(minimum 800 µl).
Program the following methods and series on the autosampler:
Method 3-7
Injection mode: Partial loop fill
Flush volume: 30 µl
Injections/vial: 3
Inj. volume: 10 µl (20, 30, 40, 50 µl)
Analysis time: 1 min
Wash: None
Series 5-9
=
∑
i
∑
i
Peakarea
3
Peakarea
i
3
i Eluent
Testsolution
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