Smartline Autosampler 3900 Manual / Handbuch V 1508 03/2007
Smartline Autosampler 3900 Manual / Handbuch V 1508 03/2007
Smartline Autosampler 3900 Manual / Handbuch V 1508 03/2007
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42 Operation<br />
Mix program<br />
After pressing the AS <strong>3900</strong> will ask if the mix program must be<br />
used in this method if is pressed AS <strong>3900</strong> returns with the first<br />
screen of the mix programming.<br />
If is pressed the mix program is not used in this method and<br />
the AS <strong>3900</strong> returns to the previous screen. The content of the mix<br />
program is NOT removed and will be available if the next time<br />
is pressed.<br />
METHOD NUMBER: 1 SECTION: MIX LINE:01<br />
END OF MIX<br />
<br />
The mix method can contain a maximum of 15 programmable lines<br />
(including the END OF MIX).<br />
To scroll through the program steps use the and soft<br />
function keys.<br />
Press to delete the displayed step.<br />
Press [Enter] to edit the displayed step.<br />
Press to insert a step before the displayed step.<br />
METHOD NUMBER: 1 SECTION: MIX STEP:01<br />
SELECT ACTION:<br />
<br />
Three action types can be programmed , and <br />
: xxx µl from <br />
<br />
<br />
<br />
to <br />
<br />
Aspirate a programmed amount of xxx µl (max. = the syringe volume)<br />
from the sample vial, , a reagent vial, or<br />
or from the wash solvent bottle, , and dispense it<br />
into the sample vial, , or the destination vial,<br />
.<br />
To prevent cross contamination the AS <strong>3900</strong> will aspirate an<br />
additional volume of 25% of the programmed volume to flush the<br />
tubing and needle.<br />
The aspirate and dispense speed depends on the selected syringe<br />
and syringe speeds; programmed in the System Settings, See page<br />
34.<br />
If the mix is programmed the reagents and transport solvent are<br />
positioned on the following places on the tray:<br />
Transport solvent: vial 85<br />
Reagent A: vial 86<br />
Reagent B: vial 87