Smartline Autosampler 3900 Manual / Handbuch V 1508 03/2007

8 Quick Start AS 3900
Press keys: Description:
[1] [Enter] Enter Method number:1
[1] [Enter] Enter First sample vial: 01
[1] [Enter] Enter Last sample vial: 01
Press [Escape] (twice) to go back to the Ready screen
Running the Series (See page 45 of this manual)
Put a sample vial in position 1 and a vial with transport solvent (mobile
phase) in position 85.
Be sure the transport vial is filled correctly before starting a new
Series.
Press keys: Description:
[Start/Stop]
[1] [Enter] Start at Series number:1
[1] [Enter] Stop after Series number: 1
If you press AS 3900 will start searching for the transport vial
and perform 3 injections of 10 µl. After every injection the needle system
will be washed with 1 syringe volume.
EXAMPLE 3: Performing a 1:10 dilution followed by a 10 µl partial loop fill
injection
The mix method will perform the following:
Transfer 360 µl from Reagent A to the destination vial, add 40 µl sample,
mix 3 times with 250 µl and subsequently inject 10 µl.
From the Ready screen:
Programming the method (See page 38 of this manual)
Injection Program:
Press keys: Description:
[Methods] [1] [Enter] Enter Method number 1.
Selecting partial loop fill injection program
[Enter]
[30] [Enter] Flush volume: e.g. 30 µl
[1] [Enter] Number of injections per vial: 1
[10] [Enter] Injection volume: 10 µl
[100] [Enter] Enter the analysis time e.g. 1 minute
Press [Escape] once to go up one level.
Wash Program:
Press keys: Description:
Selecting Wash program
Disable the wash
Press [Escape] once to go up one level.
Mix Program: (See page 42 of this manual)
Press keys: Description:
Selecting Mix program
Confirm the use of the Mix program
[180] [Enter]
[Enter]
[Enter]
Insert the first Mix step:
Add 180 µl Reagent–A to the Destination
vial.
Insert the second Mix step: