Smartline Autosampler 3900 Manual / Handbuch V 1508 03/2007

APPENDIX C: Test Procedure 67
uAU
500000
400000
300000
200000
100000
0
-100000
UV-Detektor[1]
MIDAS_Reproducibility
9 10 11 12 13 14 15 16 17 18 19 20 21
Fig. 52: Sample volume reproducibility
Minutes
The mean of the integrated peak area is developed. Accordingly, the
variation coefficient VK1 is calculated. The calculation can be made using
the following formulas:
Peakareas
∑
=
n
∑
i=
1
Peakareas
n
i
(mean)
( Peakareai 2
− Peakareas)
σ n−1
=
n −1
, whereby i = 1 - 9
(standard deviation)
VK
1
σ
peakarea
n−1
[ % ] =
× 100
The variation coefficient should be VK = 0.5%.
Record the results on the test report form.
(variation coefficient)
2. Sample Carry-Over Percent
The system settings and the analytical parameters are determined as
explained in test 1.
To determine the sample carry-over percent, 10 µl of sample solution
(vial position 1) is alternately injected with 10 µl eluent (vial position 2)
from vials 1 and 2.
In order to do this a method (no. 2) and 3 series (no. 2-4) must be
programmed. Take into consideration that the wash function for the
injection needle must be activated in between injections.
Program the following methods and series on the autosampler:
Method 2
Injection mode: Partial loop fill
Flush volume: 30 µl
Injections/vial: 1
Inj. volume: 10 µl
Analysis time: 1 min
Wash: Yes
500000
400000
300000
200000
100000
0
-100000
uAU