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Smartline Autosampler 3900 Manual / Handbuch V 1508 03/2007

Smartline Autosampler 3900 Manual / Handbuch V 1508 03/2007

Smartline Autosampler 3900 Manual / Handbuch V 1508 03/2007

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Maintenance 55<br />

Syringe<br />

• Check the needle penetration depth, see below and adjust if<br />

necessary.<br />

• Perform a wash routine to clean the new needle by pressing<br />

<br />

Air needle replacement<br />

To replace the air needle proceed as follows (see Fig. 44):<br />

• Remove sample needle as described.<br />

• Remove air needle (10) and install new air needle.<br />

• Reinstall sample needle as described.<br />

Sample needle penetration depth<br />

To keep rest volume small, the sample needle should stop close to the<br />

bottom of the sample vial. If the needle penetration depth needs<br />

adjustment, proceed as follows (see Fig. 45):<br />

• Open keyboard cover.<br />

• Place an uncapped sample vial in position 2 of the tray.<br />

• Lower the sample needle in the vial, therefore:<br />

Program a method with a full loop injection and an analysis time of<br />

at least 10 seconds.<br />

Program a Series with the programmed method and vial 2 as first<br />

and last vial.<br />

Start the Series.<br />

As soon as the sample needle has entered the vial, switch of the<br />

power supply.<br />

• Loosen adjustment screw one turn (counter clockwise).<br />

• Use the needle connection nut (4) as a grip, adjust needle<br />

penetration depth.<br />

Needle should stop not less than 1 mm above the bottom of a<br />

standard vial and not less than 2 mm above the bottom of a conical<br />

vial.<br />

• Fasten adjustment screw (clockwise).<br />

• Re-apply the power, the AS <strong>3900</strong> will initialize and is ready for use.<br />

The AS <strong>3900</strong> is factory equipped with a 250 µl syringe, but can also be<br />

equipped with a 1000 µl syringe.<br />

To install another syringe proceed as follows:<br />

Do not disconnect the power supply of the AS <strong>3900</strong>, it is needed to<br />

move the syringe!

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