Smartline Autosampler 3900 Manual / Handbuch V 1508 03/2007

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Maintenance 53 5 µl of AIR is injected together with the sample, in case an air segment is selected in the System Settings. Sometimes other combinations of syringe, loop and/or buffer are advised: Injection volumes smaller than 5 µl: Partial loop fill: Preferably install a 20 µl sample loop to avoid loss of accuracy due to expansion of the loop content when switching from inject to load position prior to sample loading. Especially when working with high pressure (200 bar), this loss may be in the order of 0.1 - 0.5 µl for a 100 µl loop. Note that the minimum sample loss in partial loop fill mode is 30 µl (recommended minimum flush volume) for the first injection and an additional 15 (always half the programmed flush volume) for additional injections from the same vial. If a wash between injections has been programmed, sample loss is 30 µl for every injection. For zero sample loss injections, use the µl-pick injection mode. µl Pick-up: Do not install a smaller sample loop! The sample plug is transported into the loop, preceded by a programmable air segment of 5 µl, with a plug of transport liquid, which equals 2.5 times the programmed needle tubing volume. The 20 µl loop is too small to guarantee quantitative injection. Injection volumes up to twice the standard: With the standard 250 µl syringe, standard needle with tubing (15 µl) and standard 500 µl buffer, but with a 200 µl sample loop, the maximum injection volumes are: Full loop: 200 µl Sample loss remains 230 µl since loops > 100 µl need only one loop volume overfill Partial loop fill: 100 µl µl Pick-up: 77 µl Smaller volumes than 10 µl may be injected, but reproducibility and accuracy may not be < 0.5% for partial loop fill or < 1% for µl pickup! For volumes larger than 200 µl: Install the 2000 µl buffer; install the appropriate sample loop size and the appropriate syringe: Syringe volume > 2 x injection volume. Injection volumes larger than 500 µl are possible, but the sample may contaminate the syringe. Program sufficient wash after use! Buffer tubing: Table 7: Syringe and buffer tubing. Syringe Buffer tubing 250 µl 500 µl buffer tubing 1000 µl 2000 µl buffer tubing
54 Maintenance Sample needle 1 Ferrule 9 Needle holder 2 Nut 10 Air needle 3 Tefzel tubing 11 Vial sensor 4 Needle connection nut 12 Needle penetration depth 5 Ferrule (PTFE) adjustment screw 6 Conical ring 13 Air tubing to provide 7 Sample needle headspace pressure 8 Silicon air seal Fig. 44: Sample needle Sample needle replacement To replace the sample needle proceed as follows (see Fig. 44): • Open keyboard cover. • Place the sample needle in the front position, press , . • Loosen needle connection nut (4) that fixates the sample needle (7). • Loosen the nut (1) that connects the PTFE tubing (3) to the injection valve. • Remove sample needle by pulling it out of its fitting with the PTFE tubing that usually sticks to the needle. • Put in a new needle assembly, watch the air seal (6) is around the needle. • Tighten the needle assembly with needle connection nut (4), • Connect loose end of needle connection tubing to port 4 of injection valve. Do not tighten excessively; it may block the tubing! • Place the sample needle back in the home position and leave the maintenance mode, press and [Escape].
Page 1 and 2:
Smartline Autosampler 3900 Manual /
Page 3 and 4: 2 Maintenance 47 Injection valve 47
Page 5 and 6: 4 Wartung 121 Injektionsventil 121
Page 7 and 8: 6 Quick Start AS 3900 • After tur
Page 9 and 10: 8 Quick Start AS 3900 Press keys: D
Page 11 and 12: 10 Programming Chart Programming Ch
Page 13 and 14: 12 About AS 3900 Side-port needle:
Page 15 and 16: 14 Injection Principle 1. P2- I/O c
Page 17 and 18: 16 Injection Principle Fig. 8 Full
Page 19 and 20: 18 Injection Principle Fig. 15 Part
Page 21 and 22: 20 Installation Installation Fig. 2
Page 23 and 24: 22 Installation Fluid connections R
Page 25 and 26: 24 Installation - With a syringe v
Page 27 and 28: 26 Installation Do not use the 10-C
Page 29 and 30: 28 Installation Table 3: Connector
Page 31 and 32: 30 Operation Operation Typographic
Page 33 and 34: 32 Operation In the Ready screen th
Page 35 and 36: 34 Operation Turn the tray cooling
Page 37 and 38: 36 Operation Table 4: Syringe speed
Page 39 and 40: 38 Operation For information how to
Page 41 and 42: 40 Operation A wash comprises two p
Page 43 and 44: 42 Operation Mix program After pres
Page 45 and 46: 44 Operation Should a method consis
Page 47 and 48: 46 Operation With the Next Injectio
Page 49 and 50: 48 Maintenance • Pull the valve o
Page 51 and 52: 50 Maintenance Following is the pro
Page 53: 52 Maintenance Body Shaft Assembly
Page 57 and 58: 56 Maintenance Fig. 46: Changing th
Page 59 and 60: 58 Troubleshooting Analytical probl
Page 61 and 62: 60 Troubleshooting Fig. 50: Bad rep
Page 63 and 64: 62 APPENDIX A: List of Accessories
Page 65 and 66: 64 APPENDIX B: Error Codes Vials ER
Page 67 and 68: 66 APPENDIX C: Test Procedure Inspe
Page 69 and 70: 68 APPENDIX C: Test Procedure Serie
Page 71 and 72: 70 APPENDIX C: Test Procedure After
Page 73 and 74: 72 APPENDIX C: Test Procedure TEST
Page 75 and 76: 74 APPENDIX D: AS 3900, Preparative
Page 77 and 78: 76 APPENDIX D: AS 3900, Preparative
Page 79 and 80: 78 Hinweise zum Gebrauch des Handbu
Page 81 and 82: 80 Schnellstart des AS 3900 Beispie
Page 83 and 84: 82 Schnellstart des AS 3900 Beispie
Page 85 and 86: 84 Programmübersicht Programmüber
Page 87 and 88: 86 AS 3900, allgemeine Beschreibung
Page 89 and 90: 88 Injektionsprinzip 1. P2- I/O Ans
Page 91 and 92: 90 Injektionsprinzip Abb. 9 Vollsch
Page 93 and 94: 92 Injektionsprinzip Abb. 15 Partie
Page 95 and 96: 94 Installation Installation Abb. 2
Page 97 and 98: 96 Installation BRANDGEFAHR! VERWEN
Page 99 and 100: 98 Installation Zur Füllung der Sp
Page 101 and 102: 100 Installation Verwenden sie kein
Page 103 and 104: 102 Installation Tabelle 3: Anschlu
Page 105 and 106:
104 Betrieb Betrieb Typographische
Page 107 and 108:
106 Betrieb [Menu]: [MENU] READY (X
Page 109 and 110:
108 Betrieb Im Falle der installier
Page 111 and 112:
110 Betrieb Tabelle 4: Spritzengesc
Page 113 and 114:
112 Betrieb Injektionsvolumen des A
Page 115 and 116:
114 Betrieb Waschprogramm METHOD N
Page 117 and 118:
116 Betrieb Mischprogramm Nach dem
Page 119 and 120:
118 Betrieb Der Wert kann für lee
Page 121 and 122:
120 Betrieb Fernsteuerung Nach dem
Page 123 and 124:
122 Wartung Abb. 38: Ventileinbau
Page 125 and 126:
124 Wartung Wartung Bei normalem Ge
Page 127 and 128:
126 Wartung • Setzen Sie den Stat
Page 129 and 130:
128 Wartung Pufferschlauch: Tabelle
Page 131 and 132:
130 Wartung Abb. 46: Auswechseln de
Page 133 and 134:
132 Troubleshooting Analytische Pro
Page 135 and 136:
134 Troubleshooting Schlechte Repro
Page 137 and 138:
136 ANHANG A: Zubehör Artikelnr. B
Page 139 and 140:
138 ANHANG B: Fehlermeldungen Vials
Page 141 and 142:
140 ANHANG C: Test Prozeduren Abb.
Page 143 and 144:
142 ANHANG C: Test Prozeduren Serie
Page 145 and 146:
144 ANHANG C: Test Prozeduren 4. Mi
Page 147 and 148:
146 ANHANG C: Test Prozeduren TEST
Page 149 and 150:
148 ANHANG D: AS 3900, Prep Install
Page 151 and 152:
150 ANHANG D: AS 3900, Prep EPROM A
Page 153 and 154:
152 Gewährleistungsbedingungen War
Page 155 and 156:
154 INDEX(english) INDEX(english) A
Page 157 and 158:
156 INDEX(deutsch) INDEX(deutsch)
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Smartline Autosampler 3900 Manual / Handbuch V 1508 03/2007

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