Smartline Autosampler 3900 Manual / Handbuch V 1508 03/2007

6 Quick Start AS 3900
• After turning "ON" the power, the Ready screen appears displaying
the firmware revision number.
• Connect the drain tubing to the wash outlet on the lower front side
of the instrument.
• Fill the wash solvent bottle with distilled water/ 2-Propanol (80/20
v/v%) or mobile phase. Only water/organic solvents should be
used. Do not use any crystalline or buffer solutions; these may
block the system and cause severe damages. To avoid air bubbles
in the syringe degas the wash solvent.
• Fill the wash solvent tubing, syringe and buffer tubing by washing
the system 2 or 3 times. This is done by pressing the soft
function key from the Ready screen. Check if there are some air
bubbles trapped in the syringe, they can be removed by gently
ticking to the syringe.
• Connect your HPLC pump to port 1 of the injection valve and the
column (or capillary) to port 6 of the injection valve. Check for
leakage and let the system equilibrate for at least 5 minutes.
System settings (See page 34 of this manual)
Normally these settings are correctly installed, to check and set these
(standard) settings, proceed as follows:
Press keys: Description:
[System] Enter the System Settings
Enter the General Settings
[0100] [Enter] Volume of installed loop: 100 µl.
[015] [Enter] Volume of tubing needle ↔ valve:015 µl
[Enter] Syringe volume: 250 µl
[Enter] Set Syringe speed to Normal.
[Enter] Skip missing vials.
[Enter] Air segment: use air segment
[Enter] Headspace pressure: On
Press [Escape] twice to go back to the Ready screen
Example 1: Performing a 10 µl partial loop fill injection from vial number 1.
Performing injection routines is easy with AS 3900. First you define a
method such as the injection, mix or wash method. These methods are
stored in battery back up. Subsequently a Run sequence or Series can
be programmed in which those methods are linked to the Series of vials.
AS 3900 will eventually execute those Series.
Programming the method (See page 38 of this manual)
Injection Program:
Press keys: Description:
[Methods] [1] [Enter] Enter Method number 1.
Selecting partial loop fill injection
[Enter]
program
[30] [Enter] Flush volume: e.g. 30 µl
[1] [Enter] Number of injections per vial: 1
[10] [Enter] Injection volume: 10 µl
[100] [Enter] Enter the analysis time e.g. 1 minute
Press [Escape] once to go up one level.
Wash Program:
Press keys: Description:
Selecting wash program
Disable the wash
Press [Escape] three times to go back to the Ready screen.