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606 I. Zalud<br />

differences between vessel architecture in benign and<br />

malignant ovarian growths [22, 30]. Improved detection<br />

and classification of tumor architecture after instillation<br />

of contrast agents might contribute to better<br />

diagnostic accuracy.<br />

Pitfalls and Artifacts<br />

It is well known that artifacts can be observed during<br />

ultrasound examinations. The same is true for color<br />

and pulsed-wave Doppler images of blood flow [31,<br />

32]. Recognizing these artifacts is important so as to<br />

avoid image misinterpretation and, when possible, to<br />

overcome them by modifying the technique, the unit<br />

settings, or both. Most manufacturers have introduced,<br />

and many diagnostic units have obtained, color Doppler<br />

scanners. However, as with any new technology,<br />

color scanning is being used by many without a complete<br />

understanding of how the instrument can be optimally<br />

adjusted to provide the best possible diagnostic<br />

information. The examiner must have good working<br />

knowledge of the instrument controls that affect the<br />

color display and how these controls interact with each<br />

other. In addition, knowledge of Doppler physics, the<br />

basics of image display formats, and the hemodynamics<br />

of blood flow is essential to optimize color<br />

and pulsed-wave Doppler examinations.<br />

Two basic colors ± red and blue by convention ± are<br />

assigned to represent the direction of blood flow relative<br />

to the ultrasound transducer. This rule assumes<br />

an ideal situation, where the artery and vein lie parallel<br />

to the skin surface and are straight, long tubes with<br />

blood flow parallel to the vessel walls. In reality, vessels<br />

do not follow these rules. They do not lie parallel to the<br />

skin surface. They are branched and tortuous, and their<br />

diameters often change. The flow pattern can be complicated.<br />

The combination of these features makes interpretation<br />

of the direction of blood flow difficult under<br />

ideal circumstances. The most important tip for<br />

determining the direction of flow is always to know<br />

where the ultrasound beams are coming from and<br />

how they are intersecting the vessels.<br />

The length of the color or pulsed-wave Doppler bar<br />

represents the range of frequencies obtainable at a designated<br />

pulsed repetition frequency (PRF). The PRF is<br />

adjustable but is limited by the depth of the image. The<br />

Doppler bar is divided into maximum positive frequency<br />

and minimum frequency, with each assigned<br />

relative color or pulsed-wave Doppler assignments.<br />

The color shading can be selected by the operator in<br />

most systems. Once a ªcolor mapº is chosen, it should<br />

remain constant for each type of examination. The<br />

range of the Doppler scale is displayed as either the frequency<br />

or the velocity. The velocity values are calculated<br />

using a standard angle, usually 08. The numbers<br />

displayed are not quantitative values because most vessels<br />

examined to not lie parallel to the probe. These values<br />

are used, rather, in a qualitative manner; that is, as<br />

the numbers increase, the PRF increases and the ability<br />

do detect higher frequencies without aliasing is increased.<br />

The color frequency does not represent a peak<br />

frequency but a mean or mode frequency. The frequency<br />

displayed by the color is a single representative<br />

frequency between the maximum and minimum frequencies,<br />

as would be seen on a spectral waveform.<br />

These values should not be used for peak frequency<br />

or velocity measurements as is traditionally done with<br />

spectral waveforms.<br />

The PRF should be changed constantly throughout<br />

the examination to accommodate the changing velocity<br />

patterns that may be present in the observed pelvic<br />

vessels. As a general rule, one should be aware<br />

that if the PRF is set too high the lower frequencies<br />

may not be detected; and if the PRF is set too low<br />

the higher frequencies are aliased. Color flow is essentially<br />

a guide for pulsed-wave Doppler exploration<br />

of solid tissue perfusion (i.e., uterus and ovary and<br />

tumor vascularization). Basically, if there is no color<br />

flow, waveform analysis is not done. The presettings<br />

of the instrument may be the reason for false-negative<br />

Doppler findings.<br />

Aliasing refers to generation of one of the most<br />

common Doppler artifacts. It is a low-frequency component<br />

in the signal spectrum when the PRF of the<br />

instrument is less than two times the Doppler signal<br />

frequency. Doppler spectral waveforms display the<br />

aliased frequencies beginning at the opposite end of<br />

the scale and moving toward the zero baseline. Color<br />

Doppler sonography shows aliasing in the same manner:<br />

The aliased frequencies are displayed as the opposite<br />

color within the flow. In the display, dark color<br />

next to dark color usually indicates a directional<br />

change, but this color pattern also may occur with severe<br />

aliasing. Here the aliased frequencies are so high<br />

they go through the opposite color and cross the zero<br />

baseline into the dark shade of the original color. It<br />

is displayed as an apparent directional change and<br />

may be falsely interpreted. Aliasing occurs in areas<br />

where the frequency is increased owing to vessel obstruction<br />

or the angle of the vessels approaching 08<br />

relative to the transducer. Therefore aliasing is often<br />

mistaken for true flow phenomena (e.g., turbulence<br />

or eddying) rather than a display artifact. Increased<br />

PRF, adjusted zero baseline, and decreased image<br />

depth can help to avoid aliasing.<br />

The colors displayed can be chosen by the operator<br />

in most instruments. Usually manufacturer-preset<br />

maps are available, as is a method for creating personally<br />

selected maps. Experimentation with different<br />

maps for different studies is recommended to allow<br />

the operator to choose the most demonstrative colors

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