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Guide for the Care and Use of Laboratory Animals - Office of ...

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ENVIRONMENT, hOUSING, ANd MANAGEMENT8Waste disposal Wastewater treatment <strong>and</strong> disposal may be necessary insome facilities depending on water volume, quality, <strong>and</strong> chemical constituents.Local regulations may limit or control <strong>the</strong> release <strong>of</strong> wastewater.Pest Control Terrestrial animal pest control principles apply to aquaticsystems but, due to transcutaneous absorption, aquatic <strong>and</strong> semiaquaticspecies may be more sensitive to commonly used pest control agents thanterrestrial animals. Be<strong>for</strong>e use, an appropriate review <strong>of</strong> chemicals <strong>and</strong>methods <strong>of</strong> application is necessary.Emergency, Weekend, <strong>and</strong> holiday <strong>Care</strong> As with terrestrial species, aquaticanimals should receive daily care from qualified personnel who have a sufficientunderst<strong>and</strong>ing <strong>of</strong> <strong>the</strong> housing system to identify malfunctions <strong>and</strong>, if<strong>the</strong>y are unable to address a system failure <strong>of</strong> such magnitude that it requiresresolution be<strong>for</strong>e <strong>the</strong> next workday, access to staff who can respond to <strong>the</strong>problem. Automated monitoring systems are available <strong>and</strong> may be appropriatedepending on system size <strong>and</strong> complexity. Appropriate emergencyresponse plans should be developed to address major system failures.Population ManagementIdentification Identification principles are similar to those <strong>for</strong> terrestrialanimals. Identification criteria are based on <strong>the</strong> species <strong>and</strong> housing system.Identification methods available <strong>for</strong> use in aquatic species include fin clipping,genetic testing (Mat<strong>the</strong>ws et al. 2002; Nickum et al. 2004), identificationtags, subcutaneous injections <strong>of</strong> elastomeric or o<strong>the</strong>r materials (Nickumet al. 2004), individual transponder tags (in animals <strong>of</strong> sufficient size), <strong>and</strong>,as applicable, external features such as individual color patterns. Because itcan be difficult to individually identify some small aquatic animals throughout<strong>the</strong>ir life, group identification may be more appropriate in some situations(Koerber <strong>and</strong> Kalishman 2009; Mat<strong>the</strong>ws et al. 2002).Aquatic Animal Recordkeeping Adequate recordkeeping is necessary inaquatic system management. In general, <strong>the</strong> same st<strong>and</strong>ards used <strong>for</strong> terrestrialanimals apply to aquatic <strong>and</strong> semiaquatic species, although modificationsmay be necessary to account <strong>for</strong> species or system variations (Koerber<strong>and</strong> Kalishman 2009).Although many aquatic animals are maintained using group (vs. individual)identification, detailed animal records are still necessary. Animalin<strong>for</strong>mation that may routinely be captured, particularly in biomedicalresearch with fish, includes species; genetic in<strong>for</strong>mation (parental stockidentification, genetic composition); stock source; stock numbers in system;tank identification; system life support in<strong>for</strong>mation; breeding; deaths;

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