VETERINARy CARE 125Arndt SS, Lohavech D, van’t Klooster J, Ohl F. 2010. Co-species housing in mice <strong>and</strong> rats: Effectson physiological <strong>and</strong> behavioural stress responsivity. Horm Behav 57:342-351.Artwohl J, Brown P, Corning B, Stein S (ACLAM Task Force). 2006. Report <strong>of</strong> <strong>the</strong> ACLAM TaskForce on Rodent Euthanasia. JAALAS 45:98-105.ASR [Academy <strong>of</strong> Surgical Research]. 2009. <strong>Guide</strong>lines <strong>for</strong> training in surgical research withanimals. J Invest Surg 22:218-225.AVMA [American Veterinary Medical Association]. 2002. A Report from <strong>the</strong> American VeterinaryMedical Association Animal Air Transportation Study Group. Schaumburg, IL: AVMA.AVMA. 2007. AVMA <strong>Guide</strong>lines on Euthanasia. Schaumburg, IL: AVMA.Ayliffe GAJ. 1991. Role <strong>of</strong> <strong>the</strong> environment <strong>of</strong> <strong>the</strong> operating suite in surgical wound infection.Rev Infect Dis 13(Suppl 10):S800-S804.Balaban RS, Hampshire VA. 2001. Challenges in small animal noninvasive imaging. ILAR J42:248-262.Barahona H, Melendez LV, Hunt RD, Forbes M, Fraser CEO, Daniel MD. 1975. Experimentalhorizontal transmission <strong>of</strong> herpesirus saimiri from squirrel monkeys to an owl monkey.J Infect Dis 132:694-697.Bartley JM. 1993. Environmental control: Operating room air quality. Today’s OR Nurse15:11-18.Bateson P. 1991. Assessment <strong>of</strong> pain in animals. Anim Behav 42:827-839.Baumans V, Brain PF, Brugere H, Clausing P, Jeneskog T, Perretta G. 1994. Pain <strong>and</strong> distress inlaboratory rodents <strong>and</strong> lagomorphs. Lab Anim 28:97-112.Beamer TC. 1972. Pathological changes associated with ovarian transplantation. In: 44th AnnualReport <strong>of</strong> <strong>the</strong> Jackson <strong>Laboratory</strong>, Bar Harbor, Maine.Belkin NJ. 1992. Barrier materials, <strong>the</strong>ir influence on surgical wound infections. AORN J55:1521-1528.Berg J. 1993. Sterilization. In: Slatter D, ed. Textbook <strong>of</strong> Small Animal Surgery, 2nd ed. Philadelphia:WB Saunders. p 124-129.Bernal J, Baldwin M, Gleason T, Kuhlman S, Moore G, Talcott M. 2009. <strong>Guide</strong>lines <strong>for</strong> rodentsurvival surgery. J Invest Surg 22:445-451.Besselsen DG, Franklin CL, Livingston RS, Riley LK. 2008. Lurking in <strong>the</strong> shadows: Emergingrodent infectious diseases. ILAR J 49:277-290.Bradfield JF, Schachtman TR, McLaughlin RM, Steffen EK. 1992. Behavioral <strong>and</strong> physiologicaleffects <strong>of</strong> inapparent wound infection in rats. Lab Anim Sci 42:572-578.Brown MJ. 1994. Aseptic surgery <strong>for</strong> rodents. In: Niemi SM, Venable JS, Guttman HN, eds.Rodents <strong>and</strong> Rabbits: Current Research Issues. Be<strong>the</strong>sda, MD: Scientists Center <strong>for</strong> AnimalWelfare. p 67-72.Brown MJ, Sch<strong>of</strong>ield JC. 1994. Perioperative care. In: Bennett BT, Brown MJ, Sch<strong>of</strong>ield JC, eds.Essentials <strong>for</strong> Animal Research: A Primer <strong>for</strong> Research Personnel. Washington: NationalAgricultural Library. p 79-88.Brown MJ, Pearson PT, Tomson FN. 1993. <strong>Guide</strong>lines <strong>for</strong> animal surgery in research <strong>and</strong> teaching.Am J Vet Res 54:1544-1559.Butler TM, Brown BG, Dysko RC, Ford EW, Hoskins DE, Klein HJ, Levin JL, Murray KA,Rosenberg DP, Sou<strong>the</strong>rs JL, Swenson RB. 1995. Medical management. In: Bennett BT,Abee CR, Hendrickson R, eds. Nonhuman Primates in Biomedical Research: Biology <strong>and</strong>Management. San Diego: Academic Press. p 255-334.Callahan BM, Hutchinson KA, Armstrong AL, Keller LSF. 1995. A comparison <strong>of</strong> four methods<strong>for</strong> sterilizing surgical instruments <strong>for</strong> rodent surgery. Contemp Top Lab Anim Sci34:57-60.Capitanio JP, Kyes RC, Fairbanks LA. 2006. Considerations in <strong>the</strong> selection <strong>and</strong> conditioning<strong>of</strong> Old World monkeys <strong>for</strong> laboratory research: <strong>Animals</strong> from domestic sources. ILAR J47:294-306.
126 GUIdE FOR ThE CARE ANd USE OF LAbORATORy ANIMALSCarstens E, Moberg GP. 2000. Recognizing pain <strong>and</strong> distress in laboratory animals. ILAR J41:62-71.Chamberlain GV, Houang E. 1984. Trial <strong>of</strong> <strong>the</strong> use <strong>of</strong> masks in gynecological operating <strong>the</strong>atre.Ann R Coll Surg Engl 66:432-433.Clif<strong>for</strong>d CB, Watson J. 2008. Old enemies, still with us after all <strong>the</strong>se years. ILAR J49:291-302.Coderre TJ, Katz J, Vaccarino AL, Melzack R. 1993. Contribution <strong>of</strong> central neuroplasticity topathological pain: Review <strong>of</strong> clinical <strong>and</strong> experimental evidence. Pain 52:259-285.Conlee KM, Stephens ML, Rowan AN, King LA. 2005. Carbon dioxide <strong>for</strong> euthanasia: Concernsregarding pain <strong>and</strong> distress, with special reference to mice <strong>and</strong> rats. Lab Anim (NY)39:137-161.Conour LA, Murray KA, Brown MJ. 2006. Preparation <strong>of</strong> animals <strong>for</strong> research: Issues to consider<strong>for</strong> rodents <strong>and</strong> rabbits. ILAR J 47:283-293.Cooper DM, McIver R, Bianco R. 2000. The thin blue line: A review <strong>and</strong> discussion <strong>of</strong>aseptic technique <strong>and</strong> postprocedural infections in rodents. Contemp Top Lab Anim Sci39:27-32.Cunliffe-Beamer TL. 1983. Biomethodology <strong>and</strong> surgical techniques. In: Foster HL, Small JD,Fox JG, eds. The Mouse in Biomedical Research, vol III: Normative Biology, Immunology<strong>and</strong> Husb<strong>and</strong>ry. New York: Academic Press. p 419-420.Cunliffe-Beamer TL. 1990. Surgical techniques. In: Guttman HN, ed. <strong>Guide</strong>lines <strong>for</strong> <strong>the</strong> Well-Being <strong>of</strong> Rodents in Research. Be<strong>the</strong>sda, MD: Scientists Center <strong>for</strong> Animal Welfare. p80-85.Cunliffe-Beamer TL. 1993. Applying principles <strong>of</strong> aseptic surgery to rodents. AWIC Newsletter4:3-6.Danneman PJ, Stein S, Walshaw SO. 1997. Humane <strong>and</strong> practical implications <strong>of</strong> usingcarbon dioxide mixed with oxygen <strong>for</strong> anes<strong>the</strong>sia or euthanasia <strong>of</strong> rats. Lab Anim Sci47:376-385.Dardai E, Heavner JE. 1987. Respiratory <strong>and</strong> cardiovascular effects <strong>of</strong> halothane, is<strong>of</strong>lurane<strong>and</strong> enflurane delivered via a Jackson-Rees breathing system in temperature controlled<strong>and</strong> uncontrolled rats. Meth Find Exptl Clin Pharmacol 9:717-720.Devitt CM, Cox RE, Hailey JJ. 2005. Duration, complications, stress, <strong>and</strong> pain <strong>of</strong> open ovariohysterectomyversus a simple method <strong>of</strong> laparoscopic-assisted ovariohysterectomy indogs. JAVMA 227:921-927.DOI [Department <strong>of</strong> <strong>the</strong> Interior]. 2007. Revision <strong>of</strong> Regulations Implementing <strong>the</strong> Conventionon International Trade in Endangered Species <strong>of</strong> Wild Fauna <strong>and</strong> Flora (CITES) (50 CFRParts 10, 13, 17, <strong>and</strong> 23). Available at www.fws.gov/policy/library/2007/07-3960.pdf;accessed April 8, 2010.Dubner R. 1987. Research on pain mechanisms in animals. JAVMA 191:1273-1276.FASS [Federation <strong>of</strong> Animal Science Societies]. 2010. Transport. In: <strong>Guide</strong> <strong>for</strong> <strong>the</strong> <strong>Care</strong> <strong>and</strong> <strong>Use</strong><strong>of</strong> Agricultural <strong>Animals</strong> in Research <strong>and</strong> Teaching, 3rd ed. Champlain, IL: FASS. p. 54.Field K, Bailey M, Foresman LL, Harris RL, Motzel SL, Rockar RA, Ruble G, Suckow MA. 2007.Medical records <strong>for</strong> animals used in research, teaching <strong>and</strong> testing: Public statement from<strong>the</strong> American College <strong>of</strong> <strong>Laboratory</strong> Animal Medicine. ILAR J 48:37-41.Flegal MC, Fox LK, Kuhlman SM. 2009. Principles <strong>of</strong> anes<strong>the</strong>sia monitoring: Respiration. JInvest Surg 22:452-454.Fox LK, Flegal MC, Kuhlman SM. 2008. Principles <strong>of</strong> anes<strong>the</strong>sia monitoring: Body temperature.J Invest Surg 21:373-374.Gentry SJ, French ED. 1994. The use <strong>of</strong> aseptic surgery on rodents used in research. ContempTop Lab Anim Sci 33:61-63.Hackbarth H, Kuppers N, Bohnet W. 2000. Euthanasia <strong>of</strong> rats with carbon dioxide: Animalwelfare aspects. Lab Anim 34:91-96.
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INdEX21pain and distress, 121-122re