DIAZ_CESAR_1167D.pdf

Besides, individual BtubAJB filaments displayed treadmilling , which is the
growth at one end and the shortening at the 0ther end at the same rate. Also,
individual filaments showed dynamic instability, which is the main microtubule
assembly mechanism and consists of changes between growing and shrinking
phases at microtubule end. The high Prosthecobacter intracellular potassium
concentration suggests that the behavior of BtubA/B filaments would be a fast
assembly from a trimeric or tetrameric nucleus, to induce filaments bundles that
grows \ogitudinally within the ce\1. This means that the dynamic instability
mechanism would not be operative, because lateral interactions stabilize the
filaments, and only the treadmlilling would allow the reorganization of BtubAIB
filaments within (inside) the cell. These results support the hypothesis that
BtubAIB bundles probably plays a structural support in Prosthecobacter that
could contribute to the elongated shape of the cells.
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