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Tackling the future challenges of Organic Animal Husbandry - vTI

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RAHMANN G & GODINHO D (Ed.) (2012): <strong>Tackling</strong> <strong>the</strong> Future Challenges <strong>of</strong> <strong>Organic</strong> <strong>Animal</strong> <strong>Husbandry</strong>.<br />

Proceedings <strong>of</strong> <strong>the</strong> 2 nd OAHC, Hamburg/Trenthorst, Germany, Sep 12-14, 2012<br />

Material and methodology<br />

The experiment was conducted at <strong>the</strong> Research Centre Foulum, Aarhus University, Denmark. The<br />

study used a 2 x 2 factorial design – with two genotypes and two dietary treatments (supplemented<br />

with A. annua dried leaves or not) as main factors – and with three replicates for each treatment<br />

combination. In total, <strong>the</strong> experiment thus included 12 flocks <strong>of</strong> 35 broilers <strong>of</strong> mixed sex. During<br />

<strong>the</strong> study, animals were fed a typical organic concentrate, whole wheat and supplemented with water.<br />

30 broilers were randomly allocated to 12 plots outdoor at 29 days <strong>of</strong> age. Six groups, three <strong>of</strong><br />

each genotype, were fed Artemisia mixed in diet while <strong>the</strong> non-treated groups received diet without<br />

A. annua. 60 broiler called “seeders” were naturally infected with contaminated manure according<br />

with Velkers et al. (2010) and after 10 days, at 39 days <strong>of</strong> age, 5 “seeders” were introduced to each<br />

<strong>of</strong> <strong>the</strong> 12 plots. Ten broilers called “tracers” where randomly selected per plot and monitored twice<br />

weekly for coccidia oocyst (oocysts/g faeces; OPG) and A. galli egg (eggs/g faeces; EPG) excretion.<br />

In <strong>the</strong> end <strong>of</strong> <strong>the</strong> study, at 74 days <strong>of</strong> age, 5 broilers per plot (n=60) (not “seeders” and not<br />

“tracers”) were harvested for lesion score in accordance with Johnson and Reid (1970) and for<br />

counting nematodes in <strong>the</strong> intestinal tracts. Consumption <strong>of</strong> diet was recorded twice a week and a<br />

mean individual feed consumption was calculated for both genotypes. All broilers in this study were<br />

weighed individually in four specific moments at different ages. Analysis <strong>of</strong> variance was performed<br />

using <strong>the</strong> MIXED procedure in SAS for infection and performance attributes. Total weight<br />

gain and <strong>the</strong> daily weight gain were calculated individually while feed conversion rate was calculated<br />

per plot. More information on methods can be found at Almeida et al. (2012).<br />

Results<br />

The overall body weight gain and <strong>the</strong> weight gain when coccidia infection was subdued showed a<br />

three-way interaction among genotype, sex and treatment (Table 1). Weight gain was higher when<br />

K8R females were supplemented with A. annua whereas supplemented K8R males had a lower<br />

weight gain than non-supplemented males (Table 2). No differences for <strong>the</strong> overall performance <strong>of</strong><br />

flocks treated and untreated were reported at <strong>the</strong> end <strong>of</strong> <strong>the</strong> trial.<br />

Table 1. Levels <strong>of</strong> significance <strong>of</strong> factors influencing performance and infection<br />

Weight Gain<br />

(Kg)<br />

Daily gain (g/d) periods a Mean OPG b A. galli larvae<br />

c<br />

29-42 42-56 56-74<br />

Treatment NS * NS * * NS<br />

Genotype ** ** ** ** NS NS<br />

Genotype x Treat. NS NS NS NS NS NS<br />

Sex ** ** ** ** * -<br />

Treatment x Sex * NS NS * NS -<br />

Genotype x Sex * NS NS * NS -<br />

Treat. x Gen. x Sex * NS NS * NS -<br />

NS = Non Significant; * significant at P

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