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Meeting the Challenge of Yellow Rust in Cereal Crops - ICARDA

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234<br />

Comparison <strong>of</strong> reactions <strong>of</strong> some wheat genotypes at adult<br />

plant stage to Pucc<strong>in</strong>ia striiformis f.sp. tritici.<br />

F. Afshari, 1 M.R.J. Kamali 1 and S. Rajaei 2<br />

1. Seed and Plant Improvement Institute (SPII), Karaj, Islamic Republic <strong>of</strong> Iran;<br />

2. Agricultural and Natural Resources Research Centers <strong>of</strong> Fars, Islamic Republic <strong>of</strong><br />

Iran<br />

In order to identify resistance <strong>in</strong> genotypes <strong>of</strong> wheat at <strong>the</strong> adult plant stage, a<br />

field experiment was carried out <strong>in</strong> Karaj and Zargan <strong>in</strong> <strong>the</strong> 2005/06 cropp<strong>in</strong>g<br />

season. All 56 genotypes toge<strong>the</strong>r with a susceptible landrace (Bolani) were<br />

sown <strong>in</strong> two 1-m long rows 30 cm apart, under artificial <strong>in</strong>oculation with race<br />

134E134A+ <strong>in</strong> Karaj and race 166E134A+SK+ on <strong>the</strong> Zargan experimental field<br />

station. The first pathotype has shown virulence on plants with Yr2, Yr6, Yr7,<br />

Yr9 and YrA, and <strong>the</strong> second pathotype has recently become dom<strong>in</strong>ant <strong>in</strong><br />

central Iran (Fars prov<strong>in</strong>ce), with virulence on plants with genes Yr2, Yr6, Yr7,<br />

Yr9, YrA, YrSD and Yr27(SK). A susceptible wheat landrace, Bolani, was<br />

planted as spreader around <strong>the</strong> field experiment and among <strong>the</strong> rows. The<br />

response <strong>of</strong> plants was evaluated us<strong>in</strong>g a modified Cobb’s Scale, at flag leaf<br />

appearance. In <strong>the</strong> field, 52 genotypes were resistant to pathotype 134E134A+.<br />

Only 18 genotypes showed resistance to both pathotypes. The rema<strong>in</strong><strong>in</strong>g l<strong>in</strong>es<br />

and cultivars were scored moderately susceptible to susceptible. These<br />

resistant l<strong>in</strong>es will be ei<strong>the</strong>r released as new cultivars or used as sources <strong>of</strong><br />

resistance <strong>in</strong> <strong>the</strong> national wheat breed<strong>in</strong>g programmes.<br />

Microsatellite-based detection <strong>of</strong> stripe [yellow] rust<br />

resistance gene Yr24 <strong>in</strong> cv. Arrivato<br />

S. Bhavani, 1 R.A. Hare 2 and H. S. Bariana 1<br />

1. University <strong>of</strong> Sydney Plant Breed<strong>in</strong>g Institute, Cobbitty, Camden, Australia;<br />

2. New South Wales Department <strong>of</strong> Primary Industries, Agricultural Research<br />

Institute, Calala Lane, Tamworth, Australia<br />

The durum cv. Arrivato exhibits high levels <strong>of</strong> stripe [yellow] rust resistance.<br />

Genetic analysis based on 198 F 3 families derived from a cross <strong>in</strong>volv<strong>in</strong>g<br />

Arrivato and <strong>the</strong> susceptible parent Bansi <strong>in</strong>dicated digenic segregation for<br />

stripe rust response <strong>in</strong> <strong>the</strong> seedl<strong>in</strong>g stage. S<strong>in</strong>gle-gene segregat<strong>in</strong>g populations,

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