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Meeting the Challenge of Yellow Rust in Cereal Crops - ICARDA

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379<br />

Investigation <strong>of</strong> genetic resistance to yellow rust disease <strong>in</strong><br />

some wheat cultivars<br />

A.R. Souhani Darban 1 and A.R. Razavi 2<br />

1. Department Agriculture. Azad University Branch Mashhad, Islamic Republic <strong>of</strong><br />

Iran; 2. Agricultural and Natural Resources Research Centre, Khorasan Razavi,<br />

Mashhad, Islamic Republic <strong>of</strong> Iran<br />

<strong>Yellow</strong> rust caused by Pucc<strong>in</strong>ia striiformis f.sp. tritici is an important disease <strong>of</strong><br />

wheat <strong>in</strong> many regions <strong>of</strong> <strong>the</strong> world. In Iran, severe epidemic <strong>of</strong> <strong>the</strong> disease<br />

occurred <strong>in</strong> 1993 and 1995. In our study, we conducted a genetic analysis <strong>of</strong><br />

crosses <strong>in</strong>volv<strong>in</strong>g eight cultivars (three resistant, three moderately resistant<br />

and two susceptible). The eight cultivars were crossed <strong>in</strong> half diallel. The<br />

plants <strong>of</strong> <strong>the</strong> parents, F 1, F 2, BC 1 and BC 2 were <strong>in</strong>oculated with race 4EOA+.<br />

Pustule size and pustule density were measured The experiment was<br />

conducted <strong>in</strong> <strong>the</strong> greenhouse. The chosen experimental design was a<br />

randomized complete block with three replications. Genetic analysis and<br />

heritability <strong>of</strong> partial resistance were determ<strong>in</strong>ed us<strong>in</strong>g <strong>the</strong> method <strong>of</strong> Griff<strong>in</strong>g<br />

and Haymans. The analysis <strong>of</strong> variance showed both additive genetic variance<br />

and dom<strong>in</strong>ance <strong>in</strong> different cultivars. MS(GCA)/MS(SCA) ratio for both traits<br />

were significant. The most important <strong>of</strong> <strong>the</strong> additive variance ratio is nonadditive<br />

variance. Mean degree <strong>of</strong> dom<strong>in</strong>ance for <strong>the</strong> traits pustule size and<br />

density were calculated to be 1.119 and 0.8, respectively. The three parameters<br />

m, [d] and [h] are significant. Interaction dom<strong>in</strong>ance × dom<strong>in</strong>ance for traits<br />

was significant.<br />

Recognition <strong>of</strong> four subspecies <strong>of</strong> Pucc<strong>in</strong>ia striiformis and<br />

development <strong>of</strong> real-time PCR detection assays<br />

M. Liu and S. Hambleton<br />

Eastern <strong>Cereal</strong> and Oilseed Research Centre, Agriculture and Agri-Food Canada,<br />

Ottawa, Canada<br />

In recent years, <strong>the</strong> <strong>in</strong>cidence <strong>of</strong> yellow rust <strong>of</strong> wheat has <strong>in</strong>creased <strong>in</strong> <strong>the</strong> USA<br />

and Canada, caus<strong>in</strong>g substantial losses. Early detection and differentiation<br />

from o<strong>the</strong>r wheat diseases is important for <strong>in</strong>itiat<strong>in</strong>g appropriate pest<br />

management measures. Monitor<strong>in</strong>g <strong>of</strong> air-borne spore dispersal can provide

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